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A field experiment was conducted to determine the influence of follicular profiles over 4 d prior to superovulation on superovulatory responses in terms of the number of large follicles (F; ≥ 7mm) at oestrus and in the number of the corpus luteum (CL) 7 d later. Ultrasonography was performed once daily over 4 d prior to gonadotropin treatment (day 0) and on the day of oestrus during superstimulation. Animals conventionally superovulated (400 mg Folltropin-V) between day 8 to 12 of the oestrous cycles and 88 superovulation cycles were considered in the present experiment. Data were analysed by means of the GLM procedure of the SAS. Each type of superovulatory responses was divided into three classes - low, medium, and high. The profiles of changes in the mean diameter of the largest follicle (F1) and in the mean differences between the diameters of F1 and the second largest (F2) follicle (F1-F2) during the 4 d prior to superovulation did not differ (P>0.1) among the three classes of follicular and ovulatory responses. The proportions of superovulation cycles with high follicular and ovulatory responses were 69% and 52%, respectively. The results of the present experiment suggest that high follicular or ovulatory responses are not associated with follicular profiles prior to the initiation of superovulation.
The objective of the present study was to determine the influence of nonsteroidal anti-inflammatory drugs (NSAIDs) representing different chemical groups on progesterone (P₄) production by cultured bovine steroidogenic luteal cells. The cells were enzymatically isolated from corpora lutea collected on days 8-12 of the estrous cycle. After 24 h preincubation they were incubated for 24 h with medium only (control) or stimulated with bovine luteinizing hormone – LH (100 ng/ml; positive control) or increasing concentrations (10⁻⁸ to 10⁻⁴ M) of acetylsalicylic acid, indomethacin, ibuprofen, naproxen, piroxicam, phenylbutazone, dipyrone or nimesulide. Concentartions of P₄ in the culture media were determined by enzyme immunoassay. LH significantly increased P₄ secretion, while acetylsalicylic acid and indomethacin did not affect the production of this hormone. A significant increase in P₄ secretion was observed after administration of dipyrone at all concentrations, piroxicam at concentrations of 10⁻⁸, 10⁻⁷ and 10⁻⁵ M, phenylbutazone and naproxen at concentrations of 10⁻⁷ and 10⁻⁶ M and ibuprofen at concentrations of 10⁻⁵ and 10⁻⁴ M. Nimesulide did not affect P₄ production at concentrations of 10⁻⁸ – 10⁻⁵ M, while at a concentration of 10⁻⁴ M it inhibited P₄ secretion. The results obtained indicate that NSAIDs may change the production of P₄ in bovine luteal cells, however, these changes are dependent on the substance used.
The aim of this work was to study the influence of hypokinetic conditions on the ovary and corpus luteum of pregnant rats. The rats were kept in hypokinetic conditions for 5 days in the period between the 13th and 18th days of pregnancy. A three-dimensional reconstruction of the ovary and corpora lutea and also a stereological evaluation of the luteal cells and their nuclei were performed using serially cut material. Hypokinesia caused a decrease in the mean volume of the ovary and individual corpus luteum and in the total volume of corpora lutea per ovary in immobilised animals as compared to the control. Moreover, a decrease was observed in the mean number of luteal cells and an increase in the size of these cells, as well as in the mean volume fraction of their nuclei. These results indicate that immobilisation of pregnant rats for 5 days considerably influences the morphology of the corpus luteum and luteal cells.
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