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1

Corneal endothelial cell cultures

100%
Rzymowska J., Gawron A., Swiech-Zubilewicz A., Pawlikowska-Pawlega B.
Folia Histochemica et Cytobiologica. Supplement
|
1997
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tom 35
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nr 2
2
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Experimental allogenic transplantation of cornea endothelial cells in cats

86%
Kielbowicz Z., Kuryszko J., Strzadala L.
Polish Journal of Veterinary Sciences
|
2010
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tom 13
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nr 4
Background: The aim of the present study was assessing the possibility of experimental allogenic transplantation of cat cornea endothelial cells, multiplied in vitro, into the anterior chamber of the eyeball in recipient cats. The reason for undertaking the research is the need to develop a method that would help in the cornea treatment in animals with corneal opacification following cataract surgery, as well as lens dislocation, injuries and endothelium degeneration. Methods: Cats aged 10-12 months were used in the experiment. Cornea fragments consisting of the posterior limiting membrane and posterior epithelium were placed in Iscove;s medium with addition of 10% foetal calf serum. Multiplied in vitro cells were injected into the anterior chamber of recipient cats. The cornea was subject to histological, histometric and SEM examination on the 3rd, 7th, 20th and 30th day after the surgery. Results: Micromorphological examination of the cornea showed full restitution of its endothelium 30 days after transplantation. Complete regeneration of structures indispensable for normal functioning of the posterior epithelium occurred as a result of implantation. Conclusions: In this study the results show that implantation of the cells of posterior corneal epithelium of donor cats, multiplied into vitro and injected into the anterior chamber of recipient cats. The cornea regained its full function, the layer of the posterior epithelium was regenerated and the stroma stabilized, presenting the image of full and proper corneal translucency.
3
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Repair of a traumatic corneal laceration in a cat using a tectonic heterograft

86%
Ito S., Terakado K., Ichikawa Y., Zama T., Minami T., Kudo S., Kanemaki N.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
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2014
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nr 2
4

The ultrastructure of the chicken embryonal cornea and lens after caffeine administration

86%
Kujawa-Hadrys M., Tosik D., Bartel H.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2008
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tom 50
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nr 1
5

The distribution and age-related localisation of immunoreactive substance P in corneal nerve plexus of the dog

86%
Lasys V., Sienkiewicz W., Stropus R., Lakomy M., Daugnora L.
Polish Journal of Veterinary Sciences
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2000
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tom 03
|
nr 2
The aim of this study was to determine histotopography and age-related changes of immunoreactive substance P (SP-IR) nerve plexus in dog cornea. In this research corneas of three groups of dogs of different age were used: young (2-3 months), adult (1-3 years) and old (8 and more years). Immunohistochemical demonstration of SP was performed on 40 um serial free-floating corneal sections cut parallel to the corneal surface. Results showed that SP-IR nerve plexus of dog cornea was formed by thick (21.9-73 μm diameter), medium (7.3-21.8 μm diameter) and thin (2.19-7.2 um diameter) nerve bundles and varicose or smooth nerve fibers branching into terminals. From limbus SP-IR thick nerve bundles ran radially through superficial and intermediate layers of stroma to the central part of cornea. Thick nerve bundles split into medium ones in central and pericentral parts of cornea. They branched repeatedly forming nerve plexus of stroma. Thin nerve bundles located in subepithelium, superficial and intermediate layers of stroma split from thick and medium bundles. Nerve fibres branched from thin bundles, curved and joined with each other, forming a network of various forms. The highest amount of nerve fibres was observed in corneal epithelium and subepithelium layer. The nerve bundles of young dogs were smaller in diameter than those of adults and old ones. With age the density of nerve fibres and thin bundles decreased in corneal epithelium and subepithelium layer. The density of medium and thick bundles in superficial and intermediate stroma was similar in dogs of all ages. No nerve plexus elements were noticed in stromal deep layer and endothelium in dogs of all ages.
6

Aspergillus penicillioides Speg. implicated in keratomycosis

72%
Machowicz-Matejko E., Furmanczyk A., Zalewska E. D.
Polish Journal of Microbiology
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2018
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tom 67
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nr 4
The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.
7

Studies on keratoconjunctivitis sicca incidence in crossbred dogs

72%
Balicki I., Radziejewski K., Silmanowicz P.
Polish Journal of Veterinary Sciences
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2008
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tom 11
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nr 4
353-358
The present study was aimed at determination of keratoconjunctivitis sicca occurrence in crossbred dogs. The investigations covered 36 mongrel dogs with keratoconjunctivitis sicca recognized by the ophthalmic examination. Patients' age and sex was established. The ophthalmic evaluation protocol included: the conjunctiva examination (humidity, redness, discharge), the corneal examination (transparency, vessel ingrowth, pigmentation, defects), Schirmer tear test. The highest incidence among the crossbred dogs was reported between 6 and 9 year of age, males accounted for 64% of cases. In a clinical study involving 61 corneas, 10 appeared to be completely opaque. The pigmentation occured in 75% of corneas. The corneal defects were found in 50% of cases. A 10-15 mm/min Schirmer test was established in 29% while 0-5 mm/min in 28% of cases.
8
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"Indolent ulcer" in Boxer

72%
Bryla P. K.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
|
2011
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nr 3
Artykuł przedstawia przypadek leczenia wrzodu rogówki u psa rasy bokser. Wrzód ten określany jest terminem "trudnogojący się" lub "indolent ulcer". Wrzody tego typu są predylekcyjne dla niektórych ras psów. Powstają często spontanicznie i niestety często mogą nawracać. istota choroby polega na oddzielaniu się nabłonka rogówki od jej istoty właściwej. Przyczyną tego może być wada hemidesmosomów, które odpowiadają za przyleganie nabłonka do stromy lub też procesy zwyrodnieniowe stromy rogówki. W obrazie klinicznym, po wybarwieniu rogówki za pomocą fluoresceiny, widoczne jest podciekanie barwnika pod warstwy rogówki. Istnieje kilka metod leczenia tego typu owrzodzenia. wymienić należy; keratotomię, powierzchowną keratektomię, chirurgiczne usunięcie martwego nabłonka rogówki. Leczeniu chirurgicznemu zawsze towarzyszy leczenie farmakologiczne. W naszym przypadku u psa zastosowaliśmy leczenie za pomocą keratotomii kratkowej skojarzonej z leczeniem farmakologicznym.
9
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First cases of Acanthamoeba keratitis in Slovakia

72%
Ondriska F., Mrva M., Lichvar M., Ziak P., Murgasova Z., Nohynkova E.
Annals of Agricultural and Environmental Medicine
|
2004
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tom 11
|
nr 2
We present the case report of the first identification of Acanthamoeba as a causative agent of keratitis in the Slovak Republic. For the first time, Acanthamoeba sp. Group III was isolated from a 53-year-old patient with keratitis, which was manifested after an injury of the right eye. A delayed visit to a physician as well as a late diagnosis of the illness led to the advanced stage of eye disease. As the treatment with itraconazol and cornea transplantation showed no result, enucleation of the eye was decided. Acanthamoeba ludgunensis was also the causative agent of keratitis in a 39-year-old patient wearing contact lenses. His complaints occurred a month after bathing in a thermal swimming pool. The symptoms presented in the left eye were those of herpetic keratitis, and led to a cloudy cornea with circular infliltrate and poor vision. A prompt clinical and laboratory diagnosis, along with treatment with propamidine-isetionate resulted in a significant improvement of the eye condition. Contact lenses were probably related to another case of Acanthamoeba keratitis. The patient, a 15-year-old girl, kept wearing contact lenses during bathing in various swimming pools and in the sea; her contact lenses were also regularly washed under tap water. Due to the fact that cysts of Acanthamoeba sp. group II were found in the contact lens solution, this is presumed to be the source of the eye infection.
10

Cornea of Ommatidia in Lepidoptera [Insecta] - a scanning electron microscope study

72%
Razowski J., Wojtusiak J.
Folia Biologica
|
2006
|
tom 54
|
nr 1-2
11

Morphometric study of the cornea in human embryos

72%
Styszynski A., Bruska M., Wozniak W.
Folia Morphologica
|
2001
|
tom 60
|
nr 1
Morphometric study was conducted on 28, serially sectioned staged human embryos. The cornea is of equal thickness during its early development (stages 15–17). During developmental stages 18 and 19 (7th week) it is thicker in the central part. In the last embryonic week the peripheral part of the cornea becomes thicker than its central part. The performed study elucidates structural differentiation during development of the cornea.
12

The role of the innate and adaptive immune responses in Acanthamoeba keratitis

72%
Niederkorn J. Y.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
|
tom 50
|
nr 1
13

Ultrastructural changes in the developing chicken cornea following caffeine administration

58%
Kujawa-Hadrys M., Tosik M., Bartel H.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 3
14

Evaluation of corneal and conjunctival epithelium with the use of impression cytology in mixed-breed dogs diagnosed with keratoconjunctivits sicca

58%
Balicki I., Radziejewski K., Bielecki W.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 3
The study was conducted on 18 mixed-breed dogs, aged 2.5 to 10 years. All patients were diagnosed with immune- mediated keratoconjunctivitis sicca (KCS) during an ophthalmological examination. A total of 33 corneas and conjunctivae were affected by the disease. Based on the results of Schirmer's test (STT), the patients were divided into three equal groups. In group I, the STT score was within the range of 0-5 mm, in group II - 6-10 mm, and in group III - 11-14 mm. Impression cytology of the bulbar conjunctiva and cornea were performed with the use of round millipore filters applied over the examined surfaces. One disk was used to sample material from the superior and inferior temporal quadrants of the cornea and the adjacent bulbar conjunctiva, the second disk was applied on palpebral conjunctiva surface of the palpebra superior. The disks were subsequently stained using the PAS method. The cytological analysis of corneal and conjunctival epithelial cells was performed according to the 4 grade Nelson- Adams index. In the impression cytology preparations from patients with advanced stages of KCS, a significant progress of epithelial cell metaplasia in the cornea and conjunctiva was observed - classified as grade 3. It was observed also that the degree of corneal and conjunctival cell metaplasia advancement in KCS patients increased with the decrease of STT values. The presence of goblet cells was not observed in either bulbar or palpebral conjunctiva, regardless of KCS advancement. Impression cytology is a simple and non-invasive method of examining the conjunctival and corneal epithelium and allows close monitoring of the disease process in dogs diagnosed with KCS.
15

Benzalkonium chloride (BAK) induces apoptosis or necrosis, but has no major influence on the cell cycle of Jurkat cells

58%
Pozarowska D., Pozarowski P.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 2
16

Comparative structural and functional study on the eye of freshwater teleosts: Clarias gariepinus, Malapterurus electricus, Anguilla anguilla and Oreochromis niloticus

58%
El-Mansi A. A., Fouda Y. A., Sabry D. A.
Folia Biologica
|
2018
|
tom 66
|
nr 2
17

Uszkodzenia rogówki u koni: diagnostyka i leczenie

37%
Zak A., Siwinska N.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2017
|
tom 12
|
nr 1
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