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Siderophore production is an important mechanism of biological control by a number of strains of plant growth-promoting rhizobacteria. Pseudomonas fluorescens UTPF5 was originally obtained from onion field. Biochemical and physiological characteristics of this strain refer to biovar 3 of P. fluorescens. Strain UTPF5 is an effective bacterium against several phytopathogenic fungi. Pyoverdine type siderophore of this strain was isolated using XAD amberlite column. The plant growth promotion and antifungal properties of bacteria were demonstrated under greenhouse conditions in combination with Fe-EDTA, Fe-EDDHA and Zn as modulators of pyoverdine production. Amendment with zinc, Fe-EDTA and Fe-EDDHA suppressed the disease inhibition when partially used with UTPF5. 7NSK2 and its pyoverdine mutant, MPFM1, were used as reference strains the inhibition percent of which was not affect by soil amendment. Iron chelates, especially Fe-EDDHA, increased growth and chlorophyll production by plants. This effect was improved in the presence of bacterial strains. The siderophore mutant MPFM1 did not exhibit satisfactory disease inhibition and growth promotion activity. In vitro experiments showed that purified pyoverdine could decrease the fungal growth to the same extent as pyoverdine-producing strain.
This study was conducted to evaluate the effect of rhizobial population on the effectiveness of locally isolated elite isolates of Rhizobium on common bean at the major growing area of Eastern Ethiopia. The result showed significant effect of inoculation, the varieties and their interaction on nodulation, yield and yield traits, except for the number of seed per pod. Most of tested Rhizobium isolates significantly improved the nodule number and nodules dry weight in all soils regardless of rhizobial population. Significant increase in total biomass yield and grain yield of common bean was recorded with NSCBR-14, inorganic N-fertilized and NSCBR-(25)₂ treatments in soil with a high, low and moderate rhizobial population, respectively. The highest values of most of the yield traits including NN and NDW in all experimental sites was recorded with Dursitu variety but the highest values GY and TBY with Kufanzik. Hence, the indigenous rhizobial population did not affect the effectiveness of inoculation but the soil types and varieties affect the effectiveness of the isolates.
The aim of our research was to evaluate the genetic diversity among 25 commercial varieties registered in Poland and 145 landraces of Phaseolus vulgaris var. nanus Asch. (the dwarf common bean) and Phaseolus coccineus L. (the runner bean) maintained in the National Centre of Plant Genetic Resources in Radzików. An additional goal of this study was to compare the precision and efficiency of two techniques of PCR (RAPD and AFLP), used to estimation the genetic diversity of bean. The breeding varieties of bean were registered in the period between 1950 and 2000. The landraces, collected during expeditions conducted from 1985 to 1988, mainly originated from the eastern and southern part of Poland. In the plant genetic diversity research of RAPD and AFLP markers are commonly used. Complex electrophoresis pictures of DNA fragments were taken, and revealed a considerable polymorphism. The polymorphic fragments were obtained on the basis of 6 differentiating primers using the RAPD method and 15 differentiating primers using the AFLP method. P. vulgaris and P. coccineus accessions formed distinct groups. Each of the RAPD and AFLP analyses allowed for the unique distinguishing of all accessions.
The experiment was conducted in the years 2004-2006 in a private farm in the village of Frankamionka in Zamość district. There were two experimental factors: I. Cultivation methods – sole cropping and strip intercropping; and II. Tending methods – mechanical, mechanical-chemical, and chemical weed control. The subject of the study was weed infestation of the Mela variety of common bean. Beans were sown between 30 April and 5 May. Weed infestation was assessed in the last week before harvesting by determining its floristic composition and the frequency of occurrence of particular weed species, as well as the air-dry weight of weeds. The dominant weed species were Galinsoga parviflora, Echinochloa crus-galli, Chenopodium album, and Amaranthus retroflexus, which comprised 84.7% of the total number of weeds. Strip intercropping markedly reduced the number of weeds per unit area (by 50%), as well as the dry weight of their aerial parts. The most effective method of weed control was the mechanical-chemical method, which resulted in the lowest occurrence of weeds. It also significantly reduced the weight of weeds.
The study objective is to evaluate the effect of mono-ammonuim phosphate (MAP; 0, 10, and 20 mM) applied as foliar application on the growth traits, green and dry yields characteristics, leaf photosynthetic pigments, chlorophyll fluorescence, and leaf contents of nutrients of common bean (Phaseolus vulgaris L., cv. “Bronco”) plants grown under saline soil conditions. To perform this objective, two field trials were conducted at the Experimental Farm of Faculty of Agriculture, Fayoum University during the 2016 and 2017 summer seasons. The obtained results showed that, Na⁺ content was significantly declined, while the all other tested parameters such as growth characteristics (i.e., shoot length, number of leaves per plant, area of leaves per plant, and shoot fresh and dry weights), yield characteristics of green pods and dry seeds (i.e., average pod weight, number of pods per plant, pods weight per plant, dry seed weight per plant and 100-seed weight), leaf photosynthetic pigments (i.e., total chlorophylls, total carotenoids) contents and leaf chlorophyll fluorescence (i.e., Fv/Fm and PI), leaf contents of N, P, K⁺, and Ca²⁺, and the ratios of K⁺/Na⁺, Ca²⁺/Na⁺ and K⁺+Ca²⁺/Na⁺ were significantly increased by the two levels (i.e., 10 and 20 mM) of MAP compared to the controls (without MAP). The two MAP levels conferred the same results for most of the all tested parameters; particularly growth and yields characteristics, with some exceptions. Therefore, results of this study recommend using 10 mM MAP as foliar application to optimize the common bean performances in saline soils.
A field 2-factor split-plot experiment was carried out over 1998-2000 at the Pawłowice Agricultural Experiment Station. The aim of the experiment was to define the reaction of three bean cultivars ‘Longina’, ‘Małopolanka’ and ‘Mela’ cultivated for dry seeds on foliar fertilisation with boron and molybdenum and their mixture. The length of bean vegetation period depended on the cultivar and weather conditions. Over the three-year research the longest vegetation period was recorded for ‘Longina’ (121 days), and shorter – ‘Małopolanka’ (118 days) and ‘Mela’ (115 days). Morphological features, seed yield, content of organic components and crude ash in seeds were differentiated mostly by genetic factor and much less considerably by fertilisation with microelements.
The common bean has been cultivated in Slovenia for centuries, resulting in the development of numerous landraces that are still grown today. The objectives of this study were to define the genetic background and to estimate genetic diversity changes in the traditional Češnjevec landrace of the Slovenian common bean over the last 50 years of cultivation. Fourteen microsatellite loci were analyzed for the presence, number and size distribution of alleles in 231 individuals, representing 67 common bean accessions, including 19 new and five old accessions of landrace Češnjevec collected in the 1950’s and stored at the Agricultural Institute of Slovenia (AIS). In factorial correspondence analysis and UPGMA cluster analysis, Češnjevec clustered apart from both Mesoamerican and Andean gene pools. It is suggested that occasional outcrossing, adaptation to particular environmental conditions and strong selection for consumer preferences for seed types could have played a significant role in the evolution of the additional variation in the common bean in this region. Three alleles present in old Češnjevec accessions were undetected in new Češnjevec accessions. The results presented here provide a firm basis for important and informed decisions concerning further conservation strategy and the breeding program in Slovenia.
Five plant oils from sesame (Sesamum indicum), oil-palm (Elaeis guineensis), cotton (Gossypium hirsutum), castor (Ricinus communis) and maize (Zea mays) at a dosage of 5 ml/kg of common bean seeds and a control of malathion dust 2% active substance (a. s.) at a dose 0.5 g/kg of seeds were evaluated for their ability to suppress the populations of Z. subfasciatus. Castor and palm oils resulted in effective protection comparable to that of malathion. There were a significant low percentage seed damage and weight loss in seeds treated with malathion, castor and palm oils. Total number of weevils in these treatments were least, compared to other plant oils studied. All treatments did not show adverse effect on germination capability of the seeds. This study showed that it is possible to use castor or palm oils to protect common bean seeds against Z. subfasciatus infestations. These products can be obtained locally at a reasonable cost.
W pracy oceniono zróżnicowanie genetyczne 28 południowopodlaskich populacji miejscowych Phaseolus vulgaris (fasoli zwyczajnej) zebranych podczas ekspedycji terenowych, przeprowadzonych w ciągu ostatnich 20 lat. Wykonano doświadczenia z zastosowaniem kombinacji 8 starterów EcoRI i 8 starterów Msel, w celu wybrania najlepszych kombinacji starterów do analiz AFLP. Rezultaty badań pozwoliły na wybranie 12 kombinacji starterów generujących największą liczbę polimorficznych prążków na żelach poliakrylamidowych. Otrzymane wyniki rozdziału fragmentów DNA posłużyły do określenia podobieństwa badanych obiektów metodą analizy skupień w oparciu o wartości współczynnika podobieństwa Jaccarda. Uzyskane wyniki wykazały przydatność metody AFLP do odróżnienie poszczególnych populacji Phaseolus vulgaris L. i określenie ich zróżnicowania wewnątrzpopulacyjnego.
A field experiment was conducted in 2010-2012 on a private farm located in the village of Frankamionka in the administrative district (powiat) of Zamość, on brown soil with slightly acidic pH, and the average abundance of zinc and iron. The experiment was set up in a random split-plot design with four replications, with seven methods for controlling weed infestation: 1) no weeding control, 2) hand weeding control, 3) linuron (Afalon dyspersyjny 450 SC), 4) linuron + metribuzin (Afalon dyspersyjny 450 SC + Mistral 70 WG), 5) linuron + chlomazone (Harrier 295 ZC), 6) linuron + chlomazone + metribuzin (Harrier 295 ZC + Mistral 70 WG), 7) bentazon (Basagran 480 SL twice). The objective of the study was to determine the effect of weeding control methods on the content of iron and zinc in the seeds of cv. Jaś Karłowy common bean (Phaseolus vulgaris L.). The highest seed yield, on average 29.39 dt ha-1, was obtained on the plots where weeds were controlled by the application of the herbicides Harrier 295 ZC + Mistral 20 WG just after sowing. The lowest seed yield was harvested on unweeded plots – 6.77 dt ha-1 on average. Statistical analysis showed a significant effect of the weed control methods and the weather conditions in growing seasons on the content of iron and zinc in bean seeds. The lowest amount of zinc, an average of 36.11 mg kg-1, was found in beans from unweeded plots. The use of the herbicides Afalon dyspersyjny 450 SC + Mistral 70 WG, Harrier 295 ZC and Harrier 295 ZC + Mistral 70 WG significantly increased the zinc content compared to the un weeded control and manual weeding. The highest iron concentration, on average 75.12 mg kg-1, was observed in seeds from unweeded plots. Significantly less iron accumulated in beans from plots weeded manually and by application of the herbicides Harrier 295 ZC and Harrier 295 ZC + Mistral 70 WG.
Background. Legumes are usually consumed when physiologically mature, as dry seeds, however, flageolet beans seeds are also consumed immature. They are harvested when dry matter content is about 40%, pods are filled, grown, seeds succulent, showing green or light green colour and do not require lengthy thermal processing when prepared for consumption. Material and methods. The aim of this study was to evaluate vitamin C content in immature seeds of five bean cultivars harvested when dry matter content was 40%. The analysis included raw, blanched and cooked fresh seeds and three products prepared for consumption after 0, 4, 8 and 12 months of storage: frozen products obtained using the traditional method (blanching-freezing-frozen storage-cooking), frozen products obtained using a modified method (cooking-freezing-frozen storage-thawing and heating in a microwave oven), a ready-to-eat product to consumption at ambient temperature, and canned products obtained by sterilization. Results. The application of technological processes, frozen and sterilized products storage, and the preparation for consumption had a cumulative effect in retention vitamin C content on final products. Conclusion. Comparing frozen seeds obtained by modified method with seeds treated by traditional method, generally, this one could retain more vitamin C. Canned seeds retained significantly less vitamin C than other frozen products.
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