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Biofertilizers are becoming increasingly popular in many countries and for many crops, but very few studies on their effect on growth in seedling stage have been conducted in rice. The experiments were conducted under laboratory conditions due to organic manure, fertilizers and bio fertilizers were observed in all experiments. The treatments such as organic manure, chemical fertilizers and Biofertilizers alone and in combination were applied. The growth parameters viz. germination percentage, root length, shoot length, fresh weight and dry weight were measured. Similarly the pigment contents such as chlorophyll-a, b, total chlorophyll and carotenoid were also measured at seventh day after sowing. All the parameters were higher in combined application of Biofertilizers than in single application and other treatments including control.
Methanol and ethanol extract showed antibacterial activity in range of 0-11 at 10 mg/mL at against all tested bacterial species. Methanol extract with amoxicillin exhibited effective inhibition E. coli. This combination showed antagonistic effect against B. subtilis and S. aureus. Interaction of ethanol extract with amoxicillin also showed effective inhibition against E. coli and decreased activity against B. subtilis and S. aureus. Methanol extract did not show inhibition against P. aeruginosa but it enhanced activity of ciprofloxacin on combination. Ethanol extract also exhibited effective inhibition against P. aeruginosa on combination with ciprofloxacin. But antagonist effect was observed on interaction of both extract with ciprofloxacin against other bacterial species. Interaction of methanol and ceftazidime showed mostly decreased or indifferent effect against all bacterial species. Ethanol extract with ceftazidime resulted in higher inhibition against E. coli. Combination of methanol extract with erythromycin showed higher inhibition against E. coli and also very effective inhibition against S. aureus. This combination resulted in decreased activity against B. subtilis. Ethanol extract with erythromycin also showed synergistic effect against S. aureus and effective inhibition against E. coli. These results indicate that both methanol and ethanol extract has potential to enhance the combination effect with erythromycin against S. aureus and E. coli.
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