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This paper presents the occurrence, properties and origin of chromium, nickel and cobalt in environmental samples - soil, water and the atmosphere. Methods of determining the above-mentioned elements, as well as the ways of preparing environmental samples for chemical analyses were presented. The content of chromium, nickel and cobalt in the atmosphere, the naturally occurring waters and soil was shown, along with the permissible concentrations of the above elements in the three divisions of the environment legally required in Poland and other countries in the world.
Contamination of the environment with heavy metals such as Cd is a serious problem of modern world. Exposure of plants to Cd leads to oxidative stress, inhibition of respiration and photosynthesis, increased rate of mutation and, as a consequence, stunted growth and yield decrease. One of the common reactions of plants to cadmium stress is over-production of ethylene, however the exact role of this hormone in plants response to Cd is still unrecognized. The aim of the present study is evaluation of the impact of an ethylene synthesis inhibitor, Co, on the response of soybean seedlings to cadmium stress. The experiments included measurements of growth, cell viability, ethylene production and expression of genes associated with cellular signaling in soybean seedlings exposed to CdCl2 (with Cd in a concentration of 223 μM) and/or CoCl2 (with Co in concentration of 4.6 μM). Surprisingly, the results show that Co has no effect on ethylene biosynthesis, however, it affects cell viability and expression of Cd-induced genes associated with plant signaling pathways. The affected genes encode mitogen-activated protein kinase kinase2 (MAPKK2), nitrate reductase and DOF1 and bZIP2 transcription factors. The role of Co in plants response to cadmium stress and its potential use as an ethylene inhibitor is discussed.
Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were used to study the morphology and elemental composition of the conidia, phialids and hyphae of Penicillium brevicompactum grown in the presence of cobalt concentrations of 0, 50, 200, 500, 800 and 1000 ppm (mg/l). Cobalt uptake was through the hyphae, phialids and the conidia with maximum uptake being by the conidia at a concentration of 1000 ppm. EDX revealed the increase in the percentage of calcium and magnesium in the hyphae, conidia and phialids, compared to corresponding controls, accompanying the increase in cobalt uptake. Alternatively a decrease in sulfur percentage was observed. This study might reflect the possibility of using SEM-EDX as a new technique in understanding the mechanism of tolerance.
Background. Cobalt (Co) is an essential mineral required in trace quantity in the diet of fish. Although freshwater fish are capable of accumulating adequate quantity of trace minerals from the medium, Co is extremely scarce in the freshwater resources. Therefore, freshwater fish require a supplement of Co in the diet. Since information regarding requirement of Co for growth and its balance in the body of fish is poorly documented there is scope to quantify requirement of cobalt for common carp. Materials and Methods. Two separate trials were made with fingerlings of common carp, Cyprinus carpio: a growth trial made in outdoor cement tanks for 60 days and a digestibility trial made in 15-L glass aquaria in the laboratory for 7 days. Four experimental diets (average crude protein 30.94%) supplemented by four different levels of Co (0.00%, 0.05%, 0.10%, and 1.0%) were formulated and tested in these two trials. Growth was evaluated from gain in weight, specific growth rate, feed conversion ratio, and deposition of protein and lipid in the body of the fish. Apparent protein digestibility (APD) of the diets was evaluated from the proportion of chromium (Cr) and protein in the diet and faeces. Water qualities were checked every week and levels of Co in liver, kidney, gill, gut, and caudal trunk of the fish were determined by atomic absorption spectrophotometer at the end of 60 days in the growth trial. Results. Fingerlings of C. carpio fed 0.1% to 1.0% cobalt-supplemented diet (CSD) showed significantly higher growth than the control diet. Although conversion rate, weight gain and SGR were significantly higher in 0.05% CSD as compared with control diet (0.00% CSD), there was no significant difference in apparent protein digestibility (APD), apparent net protein utilization (ANPU), and deposition of crude protein in the body between fish fed control and 0.05% CSD. Fish fed 1.0% CSD showed significantly higher level of Co in different tissues as compared with other diet groups. Conclusion. It is concluded from the present study that dietary supplement of Co (0.1% to 1.0%) serves as a growth promoter for common carp. Increased growth with no additional tissue burden of Co is achieved at 0.1% dietary Co, while a higher level of supplement (1.0%) may result in increased deposition of Co in different tissues.
The aim of the study was to determine the effect of competition between heavy metal cations on sorption extent of Cu2+ and Co2+ as well as to estimate of their mobility in soils through desorption with 0.05 M CaCl2 solution. The one-component CuCl2 or CoCl2, two-component CuCl2 and CoCl2 or three-component CuCl2, CoCl2 and Pb(NO3)2, CrCl3, NiCl2, ZnCl2 or CdCl2 solutions were introduced into the columns with two grey-brown podzolic soils. Cations of Cu2+ affected non-specific and specific Co2+ sorption extent while Co2+ had a smaller effect on Cu2+ sorption. In the three-component systems Cu2+ and Co2+ sorption was mostly inhibited by Pb2+ and Cr3+ but only to a small extent by cations of other heavy metals under investigation.
The aim of the study was to investigate whether synergism of selenium and cobalt preparations occur if jointly administered to three-year-old ewes of the Suffolk breed. Four groups were formed: Se, Co, Se+Co and control, each of 15 ewes. Blood samples were taken one month before mating and 2 weeks after the second selenium injection (the 3rd month of pregnancy). The results indicate that the fertility, prolificacy and the number of twins in the Se group was the highest (87, 131 and 62 %). However, a higher number of stillborn lambs (18 and 26 %) and lower weaned rate (100 and 92%) were observed in the Se and control groups respectively. Wherefore, the best production results were achieved by the ewes of the Se+Co group (prolificacy 127% and weaned rate 118%).
In the presented study, the concentrations of some heavy metal ions in bottom sediment samples collected from streams around Yozgat-Turkey in December 1998 and October 1999 were determined by flame atomic absorption spectrometry (FAAS) after leaching with aqua regia. Copper, iron, nickel, cobalt, lead, manganese and chromium levels in the sediment samples were found in the range of 10.4-16.7 µg/g, 58.3-113.7 µg/g, 31.9-76.4 µg/g, 8.3-19.2 µg/g, 31.2-60.3 µg/g, 34.3-64.4 µg/g and 26.7-39.6 µg/g, respectively. Cadmium contents of the sediment samples were below 3 µg/g at all stations. These values were below the maximum tolerable limits set by the Water Pollution Control Regulation of the Turkish authorities. Correlations between metal concentrations were evaluated.
Concentrations of Cu, Zn, Mn, Mo and Co were determined in plant material from pot experiments including various plant species and cultivars of oat, broad beans, maize and spinach. Considerable species differences and smaller inter cultivars differences were detected in the concentration of trace elements. Diversified intensity of these elements translocation from roots to tops was observed both among species and cultivars.
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