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Number and phagocytic activity of somatic cells in mastitic milk and the efficacy of Lydium-KLP in the treatment of clinical mastitis in cows

100%
Malinowski E., Klossowska A.
Bulletin of the Veterinary Institute in Puławy
|
1997
|
tom 41
|
nr 1
Thirty-seven cows affected with clinical mastitis (51 quarters) were injected iv with a single 0.02 mg/kg dose of Lydium-KLP. Fifteen cows were injected with both Lydium-KLP at the same dose given twice at a 24-hour interval and intramammary infusion of the agent (0.2 mg per quarter). It was found that the efficacy of the agent depended on the number of somatic cells in the mastitic milk and on their phagocytic activity at the beginning of the experiment.
2

Variability among etiological agents of clinical mastitis in cows

84%
Malinowski E., Klossowska A., Lassa H.
Polish Journal of Veterinary Sciences
|
2001
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tom 04
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nr 2
3

Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis

84%
Szczubial M., Dabrowski R., Kankofer M., Bochniarz M., Albera E.
Bulletin of the Veterinary Institute in Puławy
|
2008
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tom 52
|
nr 3
The aim of the study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with clinical mastitis of various severities and with subclinical mastitis in aspect of their usefulness for the detection of mastitis in cows. The concentration of SAA was determined using the commercial ELISA kit. The activity of Cp was determined according to the Rice et al., method. The mean concentration of SAA in milk from cows with mastitis ranged from 4.47 to 322.26 µg/mL. The mean SAA concentration in milk from healthy cows was 11.67 (±7.40) µg/mL and was significantly lower (P<0.01) compared to that in milk from cows with the particular forms of mastitis. The activity of Cp in milk from cows with mastitis ranged from 3.00 to 18.83 U/g of protein. Both in clinical and subclinical mastitis the activity of Cp was significantly higher (P<0.001) compared to that of milk from cows with healthy mammary glands (1.20 ±0.42 U/g of protein). The findings revealed that both the SAA concentration and Cp activity were sensitive indicators of inflammatory processes in the udder, even those graded as mild. Their determination in milk may be a reliable and non-invasive diagnostic method to detect mastitis, particularly its subclinical form.
4

The effect of some drugs injection to pregnant heifers on blood antioxidant status

67%
Malinowski E., Smulski S., Markiewicz H., Kaczmarowski M.
Polish Journal of Veterinary Sciences
|
2004
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tom 07
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nr 2
5

Antioxidative-related activities of lactoferrin and lactoperoxidase in milk from cows with different forms of mastitis

67%
Szczubial M., Kankofer M., Dabrowski R.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Milk samples were taken from cows with acute, subacute, chronic, and subclinical mastitis and from healthy cows. The mean activity of lactoferrin (LF) in milk from mastitic cows ranged from 8.9 ±3.0 to 12.1 ±6.9 mU/g protein and was significantly lower than that in milk from healthy cows (29.5 ±15.0 mU/g protein). In group of mastitic cows the highest LF activity was found in cows with chronic mastitis, and the lowest in those with subclinical mastitis. The lactoperoxidase activity in cows with clinical and subclinical mastitis was significantly higher in comparison with healthy cows (1.3 ±1.1 mU/g protein) ranging from 5.5 ±2.6 mU/g protein in subclinical mastitic cows to 8.4 ±5.0 mU/g protein in chronic mastitic cows. Lower LF activities in cows with mastitis than in healthy animals may lead to a decreased antioxidant defence system in mastitic cows.
6

Copper status and SOD activity in blood of cows affected with clinical mastitis

67%
Kleczkowski M., Klucinski W., Jakubowski T., Fabisiak M., Dembele K.
Bulletin of the Veterinary Institute in Puławy
|
2008
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tom 52
|
nr 3
The aim of the research was to evaluate serum copper concentration and superoxide dismutase activity (SOD) in the erythrocytes of cows with clinical mastitis. Forty-four cows were divided into four groups: A, B, and C with clinical form of mastitis caused by Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli, respectively, and healthy control group D. Copper concentration was determined by the ASA method, and SOD in the erythrocytes was measured by the SOD test. A marked increase in the blood indices tested was observed in all cows with clinical mastitis. Copper concentrations were: 14.7, 12.4, and 15.9 µmol/dm³ in groups A, B, and C, respectively, and superoxide dismutase activity was 1231.1, 1109.4, and 1321.5 U/g Hb, respectively, while in the group D, the values were: 9.11 µmol/dm³ for Cu concentration and 992.2 U/g Hb for SOD activity.
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