Goose circovirus (GoCV) infections are commonly found in geese on European and Asian farms, but the role of GoCV as a causative agent of the problem in geese production has not been established yet. The aim of the study was to investigate the prevalence and molecular characteristics of circovirus infection in commercial geese farms located in South-East Poland. Tissue samples of spleen or bursa of Fabricius from 95 dead geese of different ages have been collected from 28 farms/flocks between April and September 2005. None of the clinical signs and important problems with production were reported during the time of the investigation. A polymerase chain reaction (PCR) was designed for the diagnosis of the infection. From positive samples a C1 gene fragment which encodes the capsid protein (after translation of 250 amino sizes), using two pair of primers has been amplified and a sequencing reaction was performed in both directions. The nucleotide sequences were aligned with the sequences geese circovirus strains obtained from GeneBank by BioEdit sequence alignment editor software using Clustal W multiple alignment algorithm. Prevalence of GoCV was found in 18 (18.9%) spleens or bursa of Fabricius samples from geese from 14 (35.0) flocks. All positive birds were more than 5-weeks-old. The nucleotide sequence of C1 gene fragment of Polish GoCV showed 99% to 84% identity with C1 gene sequenced by Todd et al. (accession number AJ304456 GenBank database).
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