Wyniki wyszukiwania

1

Mutagen-induced chromosome instability in farm animals

100%

Danielak-Czech B., Slota E.

Journal of Animal and Feed Sciences

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2004

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tom 13

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nr 2

257-267

2

Sister chromatid exchange in Polish White Improved goats (Capra hircus)

86%

Wojcik E., Smalec E.

Folia Biologica

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2012

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tom 60

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nr 3-4

3

The effect of environmental factors on sister chromatid exchange incidence in domestic horse (Equus caballus) chromosomes

72%

Wojcik E., Smalec E.

Folia Biologica

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2013

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tom 61

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nr 3-4

4

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Searching for in vitro biomarkers of susceptibility to prostate and cervical cancers by analysis of chromosomal instability, gamma-H2AX foci, polymorphisms in DNA repair genes and apoptosis

58%

Wegierek-Ciuk A., Arabski M., Kedzierawski P., Florek A., Solowiej D., Gozdz S., Lisowska H., Kowalik A., Kowalska M., Wojcik A., Polanska J., Lankoff A.

Journal of Pre-Clinical and Clinical Research

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2015

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tom 09

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nr 2

Introduction and objective. According to the cancer epidemiology databases, cancer is the second leading cause of death in developing countries. Moreover, the WHO predicts a continuing increase in the incidence of cancer, extending this trend well into the next several decades. Hence, it seems obvious that the prediction of cancer susceptibility and early diagnosis is an important goal for modern biomedical sciences. The aim of this study is to clarify the value of chromosomal damage, capacity for the repair of double-strand breaks (DSBs), polymorphisms in DNA repair genes, and apoptosis as prognostic markers for prostate and cervical cancer. Materials and methods. 30 prostate cancer patients and 30 cervical cancer patients were enrolled into the study. In addition, 30 healthy female donors and 30 healthy male donors served as controls. The following endpoints were investigated: frequency of micronuclei, gamma-H2AX fluorescence, XRCC1 194C>T, XRCC1 399G>A, XRCC3 IVS5–14 A>G, OGG1 326 Ser>Cys polymorphisms and apoptosis. Results. Among all tested factors, only the homozygous variant (Arg/Arg) in XRCC1 (399 Arg/Gln) was strongly associated with prostate cancer risk, and only a low apoptotic response was connected with cervical cancer risk. The presented study confirmed a positive association between the frequency of MN and increased prostate and cervical cancer risk. However, such a biomarker is not cancer specific. In addition, the information gained by analyzing the gamma-H2AX fluorescence, as well apoptosis, had no value for predicting the risk of prostate and cervical cancers. Conclusions. The final conclusion of the study is that cancer susceptibility is a complex phenotype not readily detectable in relatively small studies by functional assays or analysis of SNP in few, selected genes.

5

Strukturalna niestabilnosc chromosomow zwierzat gospodarskich

58%

Danielak-Czech B., Slota E.

Biuletyn Informacyjny. Instytut Zootechniki

|

2000

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tom 38

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nr 4

5-10

6

Ciprofloxacin inhibits proliferation and promotes generation of aneuploidy in Jurkat cells

58%

Koziel R., Szczepanowska J., Magalska A., Piwocka K., Duszynski J., Zablocki K.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 2

Ciprofloxacin is widely used in antimicrobial therapy. However it also inhibits mitochondrial topoisomerase II and therefore affects cellular energy metabolism. At a concentration exceeding 80 µg/ml ciprofloxacin induces apoptosis, while at 25 µg/ml it inhibits proliferation of Jurkat cells without any symptoms of cell death. The aim of this study was to explain the mechanisms of ciprofloxacin-evoked perturbations of the cell cycle. Human lymphoidal cells (Jurkat) were exposed to ciprofloxacin (25 µg/ml) for 4-11 days and effects of the drug on cell proliferation (light microscopy), cell cycle (flow cytometry), cell size and morphology (confocal microscopy) as well as number of chromosomes (chromosomal spread analysis) were investigated. Exposition of Jurkat cells to ciprofloxacin inhibited cell proliferation, increased proportion of cells in the G2/M-phase of the cell cycle, compromised formation of the mitotic spindle and induced aneuploidy. These observations indicate that ciprofloxacin applied at concentrations insufficient for induction of apoptosis may stop cell proliferation by inhibition of mitosis. Chromosomal instability of such cells may, at least potentially, increase a risk of cancer development.

7

Strukturalna niestabilnosc genomu przyczyna nieprawidlowosci kariotypu swin

58%

Danielak-Czech B.

Biuletyn Informacyjny. Instytut Zootechniki

|

2001

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tom 39

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nr 4

15-23

8

Unstable chromosomal regions in subfertile farm animals

58%

Danielak-Czech B., Slota E.

Annals of Animal Science

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2002

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tom 02

|

nr 2

Ograniczanie wyników

Mutagen-induced chromosome instability in farm animals

Sister chromatid exchange in Polish White Improved goats (Capra hircus)

The effect of environmental factors on sister chromatid exchange incidence in domestic horse (Equus caballus) chromosomes

Searching for in vitro biomarkers of susceptibility to prostate and cervical cancers by analysis of chromosomal instability, gamma-H2AX foci, polymorphisms in DNA repair genes and apoptosis

Strukturalna niestabilnosc chromosomow zwierzat gospodarskich

Ciprofloxacin inhibits proliferation and promotes generation of aneuploidy in Jurkat cells

Strukturalna niestabilnosc genomu przyczyna nieprawidlowosci kariotypu swin

Unstable chromosomal regions in subfertile farm animals