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Probiotic products are representatives of functional food, which constitutes a dynamically growing segment of the food market. Functional food is defined as all the products that demonstrate beneficial effects on human health surpassing that resulting only from the presence of nutrients traditionally recognized as essential. Probiotics are selected microorganisms that are also called bioactive compounds of food products. Up until the present, the major branch of the food industry in which probiotics have been applied is the milk industry. The most typical active components of probiotic products are lactic acid bacteria, particularly Lactobacillus species, and bifidobacteria. Health claims of probiotics are numerous, but include maintenance of healthy intestinal flora (e.g. as a result of their competition for the nutrients and/or the production of antimicrobials inhibiting the growth of undesirable microflora and pathogens) and the stimulation of the immune system. Research and industry experiments on the application of probiotic bacteria in meat processing have proved that raw fermented sausages are the most suitable environment for their growth. In the meat industry, like in other food industry branches, a key criteria for the selection of appropriate probiotics strains are as follows: their safety (non-pathogenicity), growth properties during processing or resistance to changing parameters of technological processes as well as characteristics that enable the attainment of the expected quality, including the desired sensory attributes of final products. The article presents the most important properties and criteria of the selection of suitable probiotic bacteria for food and problems concerning the application of probiotics in meat processing.
In 2006 and early 2007, three major infectious diseases occurred in various parts of the world. The first was the continuation in some countries of the highly pathogenic avian influenza (HPAI), due to virus subtype H5N1. The second was the first documented expansion of bluetongue (BT) in the northern hemisphere reaching a latitude as high as 52°36’41” N. The third was the reoccurrence of foot and mouth disease (FMD) in certain areas of the world. In 2006 a total of 47 countries: Africa (8), Asia (15) and Europe (24), reported HPAI outbreaks due to H5N1. This paper contains details about the global epidemic situation of this disease. The first occurrence of BT in August 2006 in the Netherlands and afterwards in other Northern European countries was related to climate changes in the direction of the greenhouse effect, enabling the colonization of this region by different Culicoides species being vectors of BT virus. The epizootic situation of this disease, particularly in Northern Europe, was characterized. FMD continued to be a major epizootic disease in the Middle East and was difficult to control because of the traditional large-scale movements of animals in this region. The article also mentions other countries where FMD was diagnosed in 2006 and 2007 such as Turkey, East Amman. Israel, the Palestinian Autonomous Territories, Egypt, Botswana, Guinea, South African Republic, Democratic People’s Republic of Korea, People’s Republic of China, Vietnam, Ecuador, Argentina, Brazil and Bolivia, and identifies its serotypes.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 07
s.459-563,rys.,bibliogr.
The article reviews the clinical symptoms and pathogenesis of Bluetongue virus (BTV) infection of domestic and wild ruminants. The clinical signs of BTV infection occur principally in sheep but typical BT signs have also been observed in cattle infected with BTV serotype 8 in North-Western Europe. BTV infection can display a variety of clinical manifestations, ranging from subclinical or mild, to acute or even fatal infections. The lesions of BT differ not only among the animal species but also within breeds of the same species. In sheep the febrile period (41-42°C) lasts about one week. Nasal discharge, salivation, hyperemia, hemorrhages of the oral and nasal mucosa are observed 24-48 hrs after onset of fever. After the next few days edema of lips, tongue, face and ears is developed. At the end of febrile period, when mouth lesions begin to heal, coronitis may occur. The pathogenesis of BTV infection is similar in all species of ruminants. After cutaneous infection of BTV, by inoculation or through the bite of a BTV-infected Culicoides vector, the virus travels along the blood vessels to the regional lymph node, the place of the first replication of BTV. The virus is then disseminated to a variety of tissues throughout the body (particularly lungs and spleen) where replication occurs principally in mononuclear phagocytic and endothelial cells, lymphocytes and other cell types. BTV replicates in endothelial cells, causing cell injury and necrosis and leading to vascular thrombosis, tissue infarction, and, consequently, to a dysfunction of organs.
The article reviews the policy of bluetongue (BT) control in Europe until 2000 and the new strategy adopted in November this year. The control strategy applied until 2000 was consistent with the Directive 92/119/EEC of 17 December 1992, which involved the use of direct control methods, such as the demarcation of a 3-km radius protection zone and a 10-km radius surveillance zone around each infected farm, as well as the slaughter of all susceptible animals on the infected farm and possibly on neighboring farms. This strategy was modified in the autumn of 2000 by the Directive 2000/75/EC, as it became obvious that the stamping-out approach was completely inadequate in dealing with vector-borne diseases, such us bluetongue or epizootic hemorrhagic disease (EHD). According to this directive, the primary strategy for BT control should be based on the strict control of the transfer of susceptible animals from zones considered infected and on vaccination in the protection zone. The areas subject to movement restrictions are defined on the basis of intensive clinical, serological and entomological surveillance. Moreover, a strict quarantine of animals and herds is applied, as well as a safe utilization of dead animals and disinfection of animals, buildings and environment with insecticides. By the Commission Decision 2008/655/WE, the strategy for BT control also includes vaccinations aimed at reducing the spread of the virus in the environment, and measures to ensure safe movement of susceptible animals between affected and free zones. A BT control policy that combines administrative methods with prophylactic vaccination of all susceptible animals, supplemented by the application of insecticides to control Culicoides midges in the environment, is currently the most effective strategy for BT eradication.
Bluetongue (BT) is an infectious, non-contagious disease of animals, especially domestic animals and wild ruminants. BT is considered endemic in wildlife in large parts of Africa and North America. Most species of wild ruminants are susceptible to BTV infection, though frequently asymptomatically. The pathogenicity of BT among wildlife ranges from asymptomatic to fatal. Wild sheep, such as as bighorn and mouflon, are susceptible to BTV infection and can develop fatal clinical disease just like domestic sheep. Clinical disease also results from experimental or natural infection of antelope, wapiti, musk, ox, bison, yak, white-tailed deer and African buffalo, whereas blesbock, mountain gazelle, roe deer, red deer and Eurasian elk do not show clinical signs after natural or experimental infection, which can only be recognized by the presence of BTV-specific antibodies or viral RNA. Some camelids are also reportedly susceptible to BTV infection. A severe clinical form of this lethal disease has been reported in naturally infected llamas, whereas an experimental infection of llamas induced antibodies against BTV, but no clinical signs were observed. No clinical signs of BT have been observed in experimentally infected dromedary camels, but all animals seroconverted, and RNA BTV was isolated from the blood during viraemia, which suggests that camels may act as a reservoir for BTV and play an important role in its transmission. Wild animals, particularly cervids (because of their wide distribution in Europe), could be used as sentinels for the surveillance of BTV.
The aim of this paper was to evaluate the present bluetongue (BT) epidemiological situation in Europe and to present the results of BT monitoring studies carried out in the Department of Foot-and-Mouth Disease of the National Veterinary Research Institute. In 2007 BT spread to the regions of Europe where it has never been observed before. The first cases of BT were reported in the United Kingdom, Denmark, Switzerland and Czech Republic. In total, 31 598 BT outbreaks caused by BTV-8 were found in Europe. Most of the outbreaks were observed between the 34th and 41st week of 2007. Moreover, in some south European countries BT outbreaks caused by serotypes 1 and 4 (Portugal and Spain) and 1, 2, 4, 9 and 16 (Italy and Malta) were found. The BT monitoring studies initiated in our laboratory in September 2006 were continued in 2007. By January 2008 about 9850 serum and blood samples collected from animals imported into Poland from European countries affected by BTV were tested. We found 15 seroreagents, all among the cattle imported from Germany. On the 5th of December, for the first time, the viral RNA was detected by real-time RT-PCR in blood samples of seroreagent from Germany. The presence of viral RNA was detected again in three samples of EDTA blood collected from German cows imported into Poland in November 2007. These animals can be a potential source of BTV in our country after culicoides midges start their seasonal activity in the environment.
Basic information, including data about the occurrence and the pathogenesis of Bluetongue (BT), have been presented. These were followed by the main topic of this review, being the characterization of the disease occurrence after August 2006 in Europe. Due to climate change and global warming due to climate change and global warming, from this date on the disease appeared for the first time in areas that include northern Europe. On account of two different virus serotypes there are currently two main ongoing epizootics of BT. One, due to BTV-8, has already spread widely; the other, due to BTV-1, is currently starting to spread northwards. It is stressed in the paper that for the first time the infection of BT spread to the north of the 50th latitude. In these areas, affected by BTV-8 serotype, the main vectors are Culicoides species belonging to the Culicoides obsoletus complex, including Culicoides dewulfi and Culicoides chiopterus. The continuation of the BT epizootic, including winter periods, is explained by the persistence of BTV within surviving adult vectors or in the infected animal - cow or sheep - or additionally, thanks to transplacental transmission from infected cows or sheep to their progeny. A chapter of the paper is devoted to veterinary legislation used in the strategy of disease control. As an essential factor in the control of the disease the use of mass vaccination against sheep and cattle BT is recommended. Possible complications observed in the use of live virus attenuated vaccines are mentioned.
Bluetongue (BT) is a vector-borne viral disease effecting ruminants caused by bluetongue virus (BTV), transmitted mainly by bites from midges of the genus Culicoides. Since the end of 20th century, BTV is endemic in several European countries and the disease is caused mainly by BTV-8 and BTV-4 infections. Bluetongue virus is characterized by high genetic diversity. To date, over 29 BTV serotypes have been documented, including recently discovered atypical serotypes BTV (25–27). The disease has a high economic impact as it causes economic losses due to animal mortality, reduced productivity and restrictions on the movement of animals. Several reports and numerous observations indicate the contribution of animal movements to the spread of BTV infections. Thus, bluetongue surveillance that includes testing of sentinel animals as well as virological testing of animals susceptible to BTV infection imported from restricted zones due to the presence of BTV is a key factor in maintaining a BT-free status.
The aim of this study was to determine of seroprevalence of bluetongue (BT) virus specific antibodies in susceptible animals imported into Poland after 15 June 2006 from Germany, the Netherlands, France and Belgium. From 1 September to 31 December 2006, 5757 samples of sera supplied from 16 voivodeships were tested by c-ELISA, and positive results were confirmed using agar gel immunodiffusion (AGID). Out of all tested sera, only 3 samples from Zachodniopomorskie Voivodeship were found to be positive for antibodies to BTV (0.05% of the total number of sera samples). The seroreagents were held on a farm in the Swidwin district but their country of origin was the Netherlands. No virus was detected by RT-PCR in EDTA blood samples taken from seroreagents. The results obtained in the National Veterinary Research Institute confirmed by the Community Reference Laboratory for BT in Pirbright (England), where antibodies against BTV-8 were detected in the supplied sera samples.
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