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In the present study four different cell cultures, derived from rabbit kidney (RK13), rat, and murine liver (WBF344 and Hepa 1c1c7) and insect origin (Sf21) were used to examine the effects of chlorpyrifos. Sf21 cells were the most sensitive to chlorpyrifos, with significant suppression of their proliferative activity ranging from 10⁻¹-10⁻⁵ M. However, significant suppression of proliferative activity also was recorded in mammalian cell cultures Hepa 1c1c7 (10⁻¹-10⁻³ M), WBF344 (10⁻¹-10⁻² M), and RK13 ( 10⁻¹ M). A cytopathic effect and LDH leakage into the medium was observed in RK 13 ( 10⁻¹-10⁻³ M) > WBF344 and Hepa 1c1c7 cells ( 10⁻¹-10⁻² M) > Sf21 ( 10⁻¹ M) compared to solvent control. Our results indicate that chlorpyrifos exposure caused a species-dependent decrease in cell proliferation and cell membrane damage.
Background. Current studies have indicated many environmental factors, such as pesticides, that cause immune system disorders through inducing changes in humoral and cellular responses thereby increasing the risk of contracting infectious diseases and cancer. The literature suggests that low exposures to certain organophosphorus pesticides stimulate the immune system, whilst high exposures result in decreased function. Precise mechanisms for the fall in immunocompetence are often unclear, however it can be predicted that the intimate interaction between the nervous and immune systems can potentially lead to toxicity. Objectives. To determine the effects of organophosphorus pesticide, chlorpyrifos that is often used in Poland, on selected immunological responses, such as immune-competent cell proportions formed experimentally in-vivo by cells of Wistar rats during subchronic exposures after 45 and 90 days. Materials and Methods. The test was carried out on ten male and ten female Wistar rats in each of three test groups, who received 3 chlorpyrifos doses for 90 days intragastrically, according to OECD guidelines (No. 401). Two control groups were given olive oil. After completion, the animals were deeply anaesthetised by a mixture of ketamine (Vetaketam) and xylazine (Vetaxym). Immuno-competent cells were profiled by a commercial monoclonal antibody method. In order to measure the dynamics of any changes, the aforementioned immunological responses were investigated after 45 days using the same procedures for obtaining the relevant biological test material. Results. Test animals exposed to chlorpyrifos had altered number of white bood cells which were either increased or decreased relative to controls after 45 and 90 days for all exposure levels used. Conclusions. The study demonstrated changes in white-blood cell (lymphocyte) response profiles, reflecting an immunomodulation although such changes were equivocal, where both suppression and stimulation were observed.
The objectives of this work were to describe the residual behaviour of chlorpyrifos (insecticide) and chlorothalonil (fungicide), applied for the protection of carrot and parsley plantations. The field tests were carried out at farms located near Rzeszów (South-Eastern Poland) on processing carrot and parsley destined for baby food production. The results obtained indicated that disappearance rate of chlorothalonil on carrot leaves was significantly faster and its half-life time was about 7 times shorter than that of chlorpyrifos. In weather conditions of 2006, chlorpyrifos residues in rape carrot and parsley roots were reduced to levels lower than MRL and even the rigorous value of 0.01 mg/kg.
The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.
Preparations containing both organophosphates and pyrethroids are commonly used in insect control. Toxicokinetic interactions between α-Cypermethrin (CM ) and Chlorpyrifos (CPF) were studied in rats. The animals were given a solution of CM or CPF in rapeseed oil at a dose of 10 mg/kg and a mixture of CM and CPF at a dose of 5 mg/kg each by an intragastric tube once a day for 28 days. The concentrations of unchanged CM and CPF were determined in blood, liver and brain by GC-ECD. Also, the concentrations of CM and CPF were individually monitored in blood after administration of their single doses to calculate toxicokinetic parameters (Tmax., Cmax., AUC ). In urine the main metabolites 3-(4’-hydroxyphenoxy)benzoic acid (4OH3PBA) and 3,5,6-trichloro-2-pyridinol (TCP) were determined by HPLC in the rats treated daily with CM , CPF or their mixture. In the animals dosed with a single insecticide, the highest concentration of CM was found in blood and of CPF in liver. In the co-exposed rats, CPF decreased in all the tissues, while CM increased particularly in liver. The excretion of 4OH3PBA following CM administration increased significantly during the exposure period, whereas in the CPF-exposed rats, TCP was excreted at the same rate. Following the co-administration of both insecticides 4OH3PBA excretion decreased, but did not influence TCP excretion. In the coexposed animals, Cmax. and AUC increased for CM, and decreased for CPF.
In the current study the influence of single compounds and concurrent exposure to popular insecticides: organophosphate (OP) – chlorpyrifos (CPF) and synthetic pyrethroid (PYR ) – α-cypermethrin (CM) on some oxidative stress parameters and cholinesterase (ChE) activity in rats was investigated. Animals received by gavage 10 mg of single compounds or 5 mg of each per kg bw daily in rapeseed oil for 14 and 28 days. Concentrations of total thiols and TBARS, activity of catalase and cholinesterase were measured in tissues. Total thiol concentrations declined in plasma in all experimental groups after 14 and 28 days, while in liver a decrease was noted after only 14 days in animals receiving CPF and after 28 days in rats treated with CM alone with a mixture of pesticides. Lipid peroxidation presented as TBARS concentration was elevated mostly after 2 weeks of exposure in brain and liver but not in plasma in all experimental groups. Catalase activity increased in erythrocytes in all groups treated with insecticides, while in liver CM administered alone reduced the activity of the enzyme. Cholinesterase was markedly depressed to a different degree in plasma and brain of animals receiving CPF alone or in combination, while CM did not significantly elevate brain ChE. The results of this study seem to indicate that CM and CPF apart from known modes of action demonstrate their toxicity also through free radical mediated mechanisms. It is also evident that CM administered with CPF does not affect the cholinesterase inhibition generated by the latter.
The strawberry root weevil (Otiorhynchus ovatus L.) is an important pest on strawberry plantations in Poland. Neonicotinoid insecticides: thiametoxam as Actara 25 WG, thiacloprid as Calypso 480 SC, and acetamiprid as Mospilan 20 SP gave good control of the strawberry root weevil on strawberry plantations. The obtained results were better or similar to those obtained with chloropiryfos, diazinon and feni- trothion. Also fipronil (phenopyrazole group) showed very high efficacy in the con­trol of strawberry root weevil.
In 2009, studies on disappearance of some insecticides and fungicides on carrot fields were carried out. In total, 60 samples of soil, carrot roots and leaves were analysed. In the first test chloropyrifos (Nurelle D 550 EC) was applied only once. Mature carrot roots from that field contained its residues at the level of 0.029±0.008 mg/kg. In the second test chlorpyrifos was applied two times (Nurelle D 550 EC and Dursban 480 EC) and thus mature carrot from that field contained its residues at the level of 0.045±0.0039 mg/kg. In the tests carried out chloropyrifos and azoxystrobin residues in carrot roots were slightly above the level 0.01 mg/kg.
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