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The study concerned an evergreen herbal plant Waldsteinia trifolia Roch. This species can be found more and more often in garden plantings in Poland. The aim of the study was to estimate the content of photosynthetic pigments, anthocyanins and dry matter as well as the permeability of cytoplasmatic membranes of Waldsteinia leaves. Plants grew in an ornamental plant collection at the premises of the Faculty of Horticulture of the Agricultural University in Kraków. The study was conducted in 2006 and 2007 in the following months: March, May, June, September, October and November. The permeability of cytoplasmatic membranes was estimated based on electrolyte leakage from leaf discs at 20°C in relation to total electrolytes in the tissue after unfreezing. The fi rst days with temperature drops down to around 0°C in autumn resulted in a decrease in chlorophyll a, b and carotenoid content, but significantly increased anthocyanin content. The ratio of chlorophyll a to band the ratio of total chlorophylls to carotenoids did not change in particular months. In both years of study, similar changes in dry matter content of the Waldsteinia leaves were observed. Dry matter content signifi cantly increased in June and November and decreased in May and October. In June and in autumn, after first slight frosts, an increase of electrolyte leakage from the leaf discs was observed. However, high dry matter content (40.3%) was accompanied by lower permeability of the cytoplasmatic membranes of the leaves after winter during March. These results have shown good adaptation of Waldsteinia trifolia plants to the climatic conditions in Poland.
Barley leaf discs maintained in dark accumulated a massive amount of putrescine (Put), lost chlorophyll and senescenced rapidly. At the same time RNase activity increased significantly. Exogenous spermidine (Spd) inhibited RNase activity, the loss of chlorophyll and degradation of the proteins from thylakoid membranes. Using SDS-PAGE and immunoblot analysis it was shown that spermidine was effective in the retardation of the loss of LHCPII observed in water-treated detached leaves. Analysis of PSII particles isolated from leaf fragments floated in water in the dark revealed the presence of Put, Spd and Spm. In spermidine treated leaves the level of this polyamine in photosystem II was above 5-fold higher than in control. The experimental findings obtained in this study provide evidence that applied spermidine interacts directly with thylakoid membranes so that they become more stable to degradation during senescence.
This study was conducted to investigate the influence of biochanin A (isoflavone characterised by estrogenic activity) upon the growth expressed as number of cells and the content of chlorophyll a and b, total carotenoids, reducing sugars, water-soluble proteins and nucleic acids (DNA, RNA) in the unicellular green alga Chlorella vulgaris, as an experimental model. Biochanin A in a concentration of 10-6 M exerted the greatest biological activity, principally, on the 6th day of cultivation, whereas during the 9th day of algal culure it had weak stimulating properties. Under the influence of 10-6 M biochanin A, an increase in the number of cells to the level of 186% and the content of water-soluble proteins to 255%, reducing sugars to 505%, in comparision with the control culture (taken to be 100%), was observed. Moreover, the content of DNA was intensively stimulated in the range of 184% and RNA content reached the value 202% in regard to the control. Among the photosynthetic pigments, stimulation of the content of chlorophyll a to the level of 191%, chlorophyll b to 180% and total carotenoids to 172%, compared with the control culture of algae devoid of biochanin A, was recorded.
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