Wyniki wyszukiwania

1

Role of chitinases in plant growth and development - effect of Arabidopsis thaliana chitinase gene function disturbances

100%

Kasprzewska A., Kacperska A.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

2

Study of wine proteins by immunological methods. II. Eevidence for structural similarity amongst the major wine proteins and structural dissimilarity with chitinase and thaumatin

100%

Picarra-Pereira M. A., Monteiro S., Loureiro V., Teixeira A., Ferreira R. B.

Polish Journal of Food and Nutrition Sciences

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1998

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tom 07

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nr 2 Suppl.

3

Effect of carbon source on the production of lytic enzymes by filamentous fungi of the Trichoderma genus

84%

Piegza M., Szlaczka K., Laba W., Witkowska D.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2014

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tom 17

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nr 2

4

Moulds lytic enzymes in the production of Yarrowia high-protein feed additives

84%

Piegza M., Jurkiewicz M., Kancelista A., Laba W., Witkowska D.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2015

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tom 18

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nr 4

5

Chitin, a key factor in immune regulation: lesson from infection with fungi and chitin bearing parasites

84%

Brodaczewska K., Donskow-Lysoniewska K., Doligalska M.

Acta Parasitologica

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2015

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tom 60

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nr 2

6

Comparative studies on the activities of chitinase, beta-1,3-glucanase, peroxidase and phenylalanine ammonia lyase in the leaves of triticale and wheat infected with Stagonospora nodorum

84%

Rybka K., Arseniuk E., Wisniewska J., Raczynska-Bojanowska K.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 1

The activities of chitinase, β-1,3-glucanase, peroxidase and phenylalanine ammonia lyase, constitutive and induced by Stagonospora nodorum were examined in the 10 – 14 day old seedlings of three triticale and two wheat cultivars under controlled environmental conditions and in flag leaves of two triticale cultivars in the field. Two S. nodorum isolates of different virulence were used. Both the constitutive and induced activities in triticale and wheat depended on genotype and in triticale the effect of growth conditions was also evidenced. The constitutive activities of chitinase, β-1,3-glucanase and peroxidase were several fold lower in flag triticale leaves in plants from the field than in the seedlings, growing under controlled conditions, but induction in the infected flag leaves was significantly more pronounced. In triticale genotypic differences in the response to infection were revealed only upon inoculation by S. nodorum isolate of higher virulence. The enzymatic activities increased several fold during successive days after the infection except for phenylalanine ammonia lyase. Induction of this enzyme was only transient and the activity decreased 48 or 96 h after infection when the activities of other enzymes were rising. In flag leaves in the field this activity was differentiated only after infection with more a virulent strain. A tendency appeared in triticale seedlings for association of the resistance to the pathogen with lower enzymatic constitutive activities. This relationship became more evident in triticale infected by S. nodorum and may imply that although the investigated enzymes are certainly involved in general, non-specific defense mechanism, they do not decide on the resistance to pathogen at least in the early stages of infection and cooperate with other factors in the complex pathogen-plant interaction. One can also assume that the enzymatic activities are associated with severity of infection rather than resistance to pathogen.

7

Occurrence and activity of the chitinolytic bacteria of Aeromonas genus

84%

Swiontek B. M., Donderski W.

Polish Journal of Environmental Studies

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2001

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tom 10

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nr 1

A study was carried out on the occurrence and activity of chitinolytic planktonic bacteria of Aeromonas sp., Aeromonas hydrophila and Aeromonas salmonicida species, isolated from lake Jeziorak. Among the identified strains decomposing chitin the most abundant were Aeromonas sp.. All the investigated strains showed maximum chitinolytic activity at pH 6.0. An increase in chitinolytic activity was observed that occurred along with temperature growth (10° - 40°C) and colloidal chitin concentration in the medium (0.5 - 2.5%). Their decomposing activity was most intense after a 192 h incubation time. No strain displayed activity after a 48 h incubation time.

8

Hemagglutinins of Beauveria bassiana strains: the effect of growth conditions on their production

84%

Peczynska-Czoch W., Wieczorek J., Urbanczyk M. J.

Archivum Immunologiae et Therapiae Experimentalis

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1992

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tom 40

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nr 5-6

9

Active chitinases in the apoplastic fluids of healthy white lupin [Lupinus albus L.] plants

84%

Burzynski A., Pislewska M., Wojtaszek P.

Acta Physiologiae Plantarum

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2000

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tom 22

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nr 1

10

Influence of physical and chemical factors on the activity of chitinases produced by planktonic bacteria isolated from Jeziorak Lake

84%

Donderski W., Trzebiatowska M.

Polish Journal of Environmental Studies

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2000

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tom 09

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nr 2

Studies on the influence of physical and chemical factors on the activity of chitinases produced by planktonic bacteria isolated from Jeziorak Lake were carried out. The results evidenced maximum activity of bacterial strains at pH 6.0 and at 10°C and 30°C. Among the strains studied, higher activity was recorded along with an increase in colloidal chitin concentration. The highest activity was recorded at 2% concentration of chitin. The majority of strains hydrolyzed chitin most intensely after 192 h of incubation, whereas no strain revealed chitinases activity after 48 h.

11

Acaconin, a chitinase-like antifungal protein with cytotoxic and anti-HIV-1 reverse transcriptase activities from Acacia confusa seeds

67%

Lam S. K., Ng T. B.

Acta Biochimica Polonica

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2010

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tom 57

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nr 3

From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC50 of 30 ± 4 μM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and was not bound by either ion exchanger. Acaconin was devoid of chitinase activity. The antifungal activity against Rhizoctonia solani was completely preserved from pH 4 to 10 and from 0ºC to 70ºC. Congo Red staining at the tips of R. solani hyphae indicated inhibition of fungal growth. However, there was no antifungal activity toward Mycosphaerella arachidicola, Fusarium oxysporum, Helminthosporium maydis, and Valsa mali. Acaconin inhibited proliferation of breast cancer MCF-7 cells with an IC50 of 128 ± 9 μM but did not affect hepatoma HepG2 cells. Its IC50 value toward HIV-1 reverse transcriptase was 10 ± 2.3 μM. The unique features of acaconin include relatively high stability when exposed to changes in ambient pH and temperature, specific antifungal and antitumor actions, potent HIV-reverse transcriptase inhibitory activity, and lack of binding by strongly cationic and anionic exchangers.

12

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Survival in extremes

67%

Malecki P. H., Rypniewski W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 1

13

Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production

67%

Quesssaoui R., Bouharroud R., Amarraque A., Ajerrar A., Mayad E. H., Chebli B., Dadi M., Elaini R., El Filali F., Walters A. S.

Journal of Plant Protection Research

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2017

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tom 57

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nr 4

The two-spotted spider mite (Tetranychus urticae Koch) is an important pest of many horticultural crops. A study was conducted to evaluate the effect of three fluorescent Pseudomonas isolates obtained from rhizospheric soil of tomato (Solanum lycopersicon) in Agadir, Morocco: Q110B, Q036B and Q172B, as potential biological control agents for T. urticae. Both acaricide and repellent activities were assessed on homogenous adult mites. The acaricidal activity test evaluated five concentrations of bacterial suspensions: 0 (control), 102, 104, 106, 108, and 1010 cfu ⋅ ml–1, while only the 1010 cfu ∙ ml−1 concentration of each bacterium was used for the repellent bioassay. The mortality rate and repellentindex were recorded 24, 48 and 72 h after application. Results indicated that the survival rate of T. urticae was reduced (p ≤ 0.01) by all three bacterial isolates compared to control. Within the 24–72 h time period the mortality rates ranged from 8 to 87%, 16 to 99%, and 13 to 89%, for Q110B, Q036B and Q172B isolates, respectively. The isolate Q036B (LC50 = 0.598 cfu ⋅ ml–1) provided higher mortality rates than Q172B and Q110B with LC50 values of 90,846 and 169,585 cfu ⋅ ml–1, respectively. Repellent activity was also the highest with Pseudomonas Q036B having a 71% repellence index at 48 h after application. Regarding the mechanism of action, all three isolates produced hydrogen cyanide, and exhibited protease and cellulose activities, although only Q036B and Q172B had potential chitinase action. Identification analysis showed that the isolates were either Pseudomonas putida (Q172B) or P. fluorescens (Q110B and Q036B). Our results indicate that the P. fluorescens isolate Q036B is a promising candidate for biological control of T. urticae, and has potential to contribute to an integrated pest management program to control this important pest. Then the fruits produced will be qualified as safe for consumers and the environment. The present work was customized to give support for policy decision makers as an agroecological potential meeting needs of industries and ecological balance.

14

Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris

67%

Golinska P., Dahm H.

Dendrobiology

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2011

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tom 65

The enzymatic activity (chitinolytic, proteolytic, pectolytic andcellulolytic) of twenty strains of Streptomyces isolated from soil and rhizosphere of Scots pine was analyzed. Most strains produced chitinases, catalyzing the degradation of chitin, the main component of fungal cell walls including fungi pathogenic for plants. This activity was about 4 times higher in the presence of Fusariumoxysporum than Rhizoctonia solani mycelium or chitin flakes. The number of the proteolytic strains was also significant. In general, rhizosphere and soil organisms synthesizedcomparable amounts of these enzymes. Over half of the analyzed Streptomyces strains produced pectolytic enzymes (polygalacturonase, pectin lyase and pectate lyase). This property was more common among rhizosphere than among soil strains. The Streptomyces strains also showedcellulolytic activity (endocellulases, exocellulases) – enzymes decomposing basic components of cell walls of plant and lower fungi (cellulose). The cellulolytic activity was differentiated and depended on the Streptomyces strain. Conclusion of our studies is that Streptomyces are the microorganisms more chitinolytic andproteolytic than pectolytic and cellulolytic.

15

Biosynthesis and properties of beta-1,3-glucanases of Trichoderma hamatum

67%

Maj A., Witkowska D., Robak M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2002

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tom 05

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nr 2

16

Utilization of shrimp-shell waste as a substrate for the activity of chitinases produced by microorganisms

67%

Swiontek B. M., Walczak M., Lalke-Porczyk E., Donderski W.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 1

177-182

Chitinolytic microorganisms such as bacteria, fungi, and actinomycetes, were isolated from the water and bottom sediments of Lake Chełmżyńskie and the soil of its watershed. The impact of various forms of chitin on the chitinolytic activity of these microorganisms was investigated. Bacteria isolated from the analyzed environments exhibited the highest chitinolytic activity in the presence of colloidal chitin. High activity was also observed in a culture medium containing shrimp shells. The analysis of chitinolytic activity of actinomycetes demonstrated that strains isolated from the soil had considerably higher activity levels than those isolated from lacustrine water and bottom sediments. All soil actinomycetes exhibited the highest activity in a culture medium containing colloidal chitin and shrimp shells. Actinomycetes collected in water and bottom sediments were characterized by similar activity levels. Chitinolytic fungi exhibited the maximum activity also in a medium containing colloidal chitin. The presence of shrimp shells in a culture medium had the lowest impact on chitinolytic activity. Among the analyzed forms of chitin, the impact of chitin powder on the activity of chitinases was the least stimulating.

17

The utilization of N-acetyloglucosamine and chitin as sources of carbon and nitrogen by planktonic and benthic bacteria in Lake Jeziorak

67%

Donderski W., Swiontek Brzezinska M.

Polish Journal of Environmental Studies

|

2003

|

tom 12

|

nr 6

It follows from the research conducted on the number of chitinolytic bacteria among planktonic and benthic bacteria that they occur in a higher number in bottom sediments than in water. However, the percentage of chitinolytic bacteria among the total number of heterotrophic bacteria was higher in the water than in the bottom sediments. Chitinolytic bacteria and the bacteria unable to decompose chitin most readily utilized N-acetyloglucosamine as additional sources of carbon and nitrogen. Chitinolytic bacteria developed well on a substrate with colloidal chitin as the only or an additional source of carbon and nitrogen. However, bacteria unable to decompose chitin displayed very weak growth on a substrate with colloidal chitin as the only source of carbon and nitrogen.

18

Plant chitinases - regulation and function

67%

Kasprzewska A.

Cellular and Molecular Biology Letters

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2003

|

tom 08

|

nr 3

The aim of this review is to present the current state of knowledge on plant chitinases and their regulation and function. Chitinases are up-regulated by a variety of stress conditions, both biotic and abiotic, and by such phytohormones as ethylene, jasmonic acid, and salicylic acid. Like other PR proteins, chitinases play a role in plant resistance against distinct pathogens. Moreover, by reducing the defence reaction of the plant, chitinases allow symbiotic interaction with nitrogen-fixing bacteria or mycorrhizal fungi. However, recent investigations have shown that these enzymes are also involved in numerous physiological events. The involvement of chitinases in development and growth processes is also described.

19

Activity of cuticle-degrading enzymes from entomopathogenic fungus Conidiobolus coronatus

67%

Czygier M., Samborski J., Dzik J. M., Walajtys-Rode E., Bogus M.

Wiadomości Parazytologiczne

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1998

|

tom 44

|

nr 3

20

Changes in the activity of peroxidase, beta-1,3-glucanase and chitinase in broad bean leaves infected with Botrytis fabae

67%

Patykowski J., Urbanek H., Malolepsza U.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

|

nr 2

Ograniczanie wyników

Role of chitinases in plant growth and development - effect of Arabidopsis thaliana chitinase gene function disturbances

Study of wine proteins by immunological methods. II. Eevidence for structural similarity amongst the major wine proteins and structural dissimilarity with chitinase and thaumatin

Effect of carbon source on the production of lytic enzymes by filamentous fungi of the Trichoderma genus

Moulds lytic enzymes in the production of Yarrowia high-protein feed additives

Chitin, a key factor in immune regulation: lesson from infection with fungi and chitin bearing parasites

Comparative studies on the activities of chitinase, beta-1,3-glucanase, peroxidase and phenylalanine ammonia lyase in the leaves of triticale and wheat infected with Stagonospora nodorum

Occurrence and activity of the chitinolytic bacteria of Aeromonas genus

Hemagglutinins of Beauveria bassiana strains: the effect of growth conditions on their production

Active chitinases in the apoplastic fluids of healthy white lupin [Lupinus albus L.] plants

Influence of physical and chemical factors on the activity of chitinases produced by planktonic bacteria isolated from Jeziorak Lake

Acaconin, a chitinase-like antifungal protein with cytotoxic and anti-HIV-1 reverse transcriptase activities from Acacia confusa seeds

Survival in extremes

Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production

Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris

Biosynthesis and properties of beta-1,3-glucanases of Trichoderma hamatum

Utilization of shrimp-shell waste as a substrate for the activity of chitinases produced by microorganisms

The utilization of N-acetyloglucosamine and chitin as sources of carbon and nitrogen by planktonic and benthic bacteria in Lake Jeziorak

Plant chitinases - regulation and function

Activity of cuticle-degrading enzymes from entomopathogenic fungus Conidiobolus coronatus

Changes in the activity of peroxidase, beta-1,3-glucanase and chitinase in broad bean leaves infected with Botrytis fabae