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Reduction of the neutralisation time during antimicrobial activity testing of disinfectants according to European standards

100%

Tyski S., Bocian E., Grzybowska W.

Roczniki Państwowego Zakładu Higieny

2013

tom 64

nr 2

Background. Evaluation of the biocidal activity of chemical disinfectants and antiseptics according to European Standards (EN) is based on determination of the reduction of the number of viable test microorganisms under defined conditions. Objective. The objective of this study was to investigate whether reducing the neutralization time required following declared product contact times for the tested microorganisms yields method validations. Material and methods. This study was conducted on 14 products containing active substances from different chemical groups: alcohols, aldehydes, biguanides, quaternary ammonium compounds, phenols, amines derivatives, oxidizing agents. These products were tested according to phase 1 tests: EN 1040:2005 and EN 1275:2005 and then according to phase 2, step 1 tests: Draft EN 13727:2005 and EN 13624:2003. Biocidal activity was evaluated using the following test organisms: S. aureus ATCC 6538, P. aeruginosa ATCC 15442, E. coli NCTC 10538, E. coli ATCC 10536, E. hirae ATCC 10541, C. albicans ATCC 10231 and A. brasiliensis ATCC 16404. Results. Validation C results for all products and tested microorganism strains were at least half of the density of the suspension for validation (Nvo) after only 10 s of neutralization. Furthermore, results from test procedures performed in parallel were also positive except 5 products toward A. brasiliensis. Conclusions. The results of our study confirm that the contact time described in the European Standards phase 1: EN 1040 and EN 1275, as well as phase 2, step 1: Draft EN 13727 and EN 13624 can be precisely determined in spite of reducing the neutralization time from 5 minutes to even 10 seconds.

Effect of different drying conditions for the viability of Candida albicans present on carriers

100%

Rohm-Rodowald E., Chojecka A., Jakimiak B., Wiercinska O., Ziemba B., Kanclerski K.

Roczniki Państwowego Zakładu Higieny

2014

tom 65

nr 1

Background. Problems in substantial under recovery of Pseudomonas aeruginosa and Candida albicans from carriers have been demonstrated for laboratories performing phase 2, step 2 efficacy tests on disinfectants relative to levels required by the EN 13697 standard. It was thus necessary to determine recoveries of these microorganisms following procedural losses incurred during drying and to optimise drying conditions such that recoveries then complied with the standard. Objectives. The aim of the study was to establish optimal drying conditions for the recovery of Candida albicans ATCC 10231 from carriers. Materials and Methods. The evaluation was performed according to the EN 13697:2001 standard procedure. A test suspension of Candida albicans and interfering substance were inoculated onto the surface of carriers (2 cm diameter stainless steel discs) and then dried under different conditions consisting of: a 37°C incubation with and without an incubator fan as well as at 23°C (room temperature) in a laminar air flow cabinet. Carriers were dried until the surfaces appeared visibly dry and the number of surviving organisms then recovered from the surface were quantified. The following were calculated for colony forming units (cfu); N (log10 cfu in a 0.05 ml test suspension), NC (the control log10 cfu in neutralizing medium), Nts (cfu numbers remaining on the surface) and the N-NC difference which should not exceed 2 log10 when microorganism recoveries are adequate and without any toxicity effects of the neutralising medium. Experiments was conducted using validating procedure (NC) which is performed with distilled water. Results. Drying at 37°C adversely affected the survival of Candida albicans and prevented the levels of microbial recovery from carriers to reach those specified by the EN 13697 standard. However, drying at around room temperatures of 23°C reduced Candida albicans mortality and increased recoveries from the carrier to levels compliant with the standard, where the N-NC differences were not greater than 2 log10. Conclusions. The viability of Candida albicans ATCC 10231 is sufficiently improved when carriers are dried at 23°C, even if the drying time exceeds 60 minutes. The density of the initial test suspension (N) should also be increased.

Antimicrobial activity of glucoprotamin-containing disinfectants

67%

Tyski S., Grzybowska W., Grzeszczuk S., Leszczynski P., Staniszewska M., Rohm-Rodowald E., Jakimiak B.

Polish Journal of Microbiology

2009

tom 58

nr 4

347-353

Introduction of a new antimicrobial agent as a drug - for treatment of infections or as a disinfectant and antiseptic, may result in the occurrence of resistance mechanisms against this agent among microorganisms. Two disinfectants of different composition - Incidin Plus for surface disinfection and Sekusept Plus for medical devices disinfection, both containing glucoprotamin as the active substance, were investigated in this study in order to analyze their antimicrobial activity. Standard bacterial and fungal strains recommended by European Standards, established by European Standardization Committee for testing bactericidal and fungicidal activity of chemical disinfectants were used in the study. Furthermore, 60 clinical bacterial strains with different susceptibility to antibiotics and chemotherapeutics, mostly multiresistant, isolated from different specimens from hospitalized patients were analyzed. In addition, 184 fungal clinical strains isolated from hospitalized patients and outpatients were also included in this study. Antimicrobial activity was evaluated according to EN 1040:2005 - using bacterial strains and according to EN 1275:2005 - using fungal strains. Glucoprotamin proved to be a very effective and rapidly acting bactericidal and fungicidal agent. Low concentration of glucoprotamin - 0.5% showed to be very effective (1 min) against clinical bacterial isolates. Incidin Plus was also very effective (5 min) against clinical fungal isolates.

Ograniczanie wyników

2 Roczniki Państwowego Zakładu Higieny

1 Polish Journal of Microbiology

2 Grzybowska W.

2 Jakimiak B.

2 Rohm-Rodowald E.

2 Tyski S.

1 Bocian E.

1 Chojecka A.

1 Grzeszczuk S.

1 Kanclerski K.

1 Leszczynski P.

1 Staniszewska M.

1 Wiercinska O.

1 Ziemba B.

1 2014

1 2013

1 2009

Wyniki wyszukiwania

Reduction of the neutralisation time during antimicrobial activity testing of disinfectants according to European standards

Effect of different drying conditions for the viability of Candida albicans present on carriers

Antimicrobial activity of glucoprotamin-containing disinfectants