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The presence of catecholamines in the nervous system of a plagiorchiid cercaria of Opisthioglyphe ranae (Frölich, 1791) is demonstrated using fluorescence methods (SPG method after De la Torre and Surgeon 1976, exciting filter of 400-410 nm max. wave length). Comparison of the distribution of catecholamines and acetylcholinesterases in the cercaria of the same species showed closely similar patterns. However, some differences in distribution were identified and these are discussed.
The ultrastructure of the proto- and paranephridial excretory systems in the metacercaria of D. pseudospathaceum is described. Basically, the protonephridial system in the metacercaria, although being more complex, does not differ in ultrastructure from that in the cercaria. All protonephridial canals have nuclei and septate desmosomes between neighbouring cells as well as desmosomes closing the canals. Contrary to the protonephridial system, the paranephridial one has a syncytial structure with few cell nuclei in the wall. No septate desmosomes were visible in cross- and longitudinal sections of the paranephridial canals and terminal pockets. The heterocellular gap junctions (nexus) between tegumental cytons and paranephridial canal walls have not previously been observed and described in diplostomid metacercaria. The possible function of these heterocellular gap junctions is discussed.
The chaetotaxy pattern of D. pseudospathaceum related to the structure of the nervous system (in the cephalic and preacetabular region) was described based upon LM and TEM investigations. The proper location of thirteen different ultrastructural types of sensory endings (SE) will be described by Czubaj and Niewiadomska (in press) were established. It was shown that the cercarial body, tail stem and furcae have a specific set of SE, and that several types of SE may be localized in different part of the cercaria. The location of particular sensory endings in the rings of the cephalic, pre- and postacetabular regions, tail stem and furcae was ascertained.
A cyathocotylid cercaria, Cercaria kuwaitae II sp. n., is described from the prosobranch gastropod, Cerithidea cingulata (Gmelin, 1790) collected from the muddy shores of Kuwait Bay. Details are presented on the morphology and behaviour of the cercaria as well as on the microtopography of the surface of the cercaria and daughter sporocyst.
Cercariae of bird schistosomes are traditionally considered to be very similar in their morphological characteristics. In order to solve the problem, we tested some methods which might be suitable for cercarial differentiation. Fourteen isolates of three Trichobilharzia species (T. szidati, T. franki, T. regenti) occurring sympatrically in Central Europe were used. Dimensions of individual cercariae do not represent a useful criterion for identification, because the intraspecific variability exceeds the interspecific one. On the other hand, chaetotaxy appears a promising way for discrimination, although some sensory papillae do not stain sufficiently with silver nitrate. The papillary pattern (i.e. number and relative position of papillae) is specific for all Trichobilharzia species studied by us. Therefore, we compiled an identification key for the three Trichobilharzia species. In addition, we tried to find species-specific surface saccharide epitopes; none of the labeled lectin probes can be used as a speciesspecific marker.
Effects of the presence of sporocysts, rediae and cercariae of Fasciola hepatica on the lipid content in the digestive gland of Lymnaea truncatula as well as on lipid levels in tissues of the parasites themselves were studied. Lipids were examined by means of histochemical and cytophotometric techniques. The snail's digestive gland lipid level was found to be almost halved in 20 days post infection; a more then 80% reduction being visible after the subsequent 40 and 60 days. The loss of lipids in the digestive gland of the infected snails point at mobilisation of lipid energy reserves to compensate for the deficiency of carbohydrates, used by these parasites. The parasite tissues such as tegument, pharynx, suckers and germ balls showed considerable lipid contents and were metabolically active. It supports the hypothesis that lipids are used as energy source by developmental stages of this parasite.
The morphology and chaetotaxy of the rediae and the cercariae of Isthmiophora melis are described and compared with other echinostomatids and related groups of trematodes. Apart from adult morphology of I. melis, which is typical of Echinostomatidae, the structure of the redia and chaetotaxy pattern of the cercaria are the most similar to those observed in an echinostomatid genus Paryphostomum as well as in representatives of the family Cathaemasiidae, especially in Cathaemasia hians.
Light and scanning electron microscopy were used to study the redial and cercarial stages of Paucivitellosus fragilis Coil et al., 1965 recorded for the first time from the prosobranch Clypeomorus bifasciata in Kuwait Bay. The redia is cylindrical with terminal mouth and furcates at the posterior end. The tegument is densely covered with short microvilli and folded into transverse muscular bands. The mouth region is surrounded by uniciliate sensory receptors and spherical bodies. The body of the extraredial cercaria is devoid of suckers and uniformly covered with microvilli, and the tail is divisible into a proximal caudal vesicle, a tail stem and broadly lobed furcae. Upon emergence from the snail, the caudal vesicle distends over the entire cercarial body terminating in a protrusible mouth with glandular lining. Only uniciliate sensory receptors and no spines are observed on body and tail of the cercaria.
The general morphology of the excretory system of the cercaria and daughter sporocyst of D. pseudospathaceum is described. The ultrastructure of the following parts was examined: in the body - the filtering complex, anterior and posterior collecting tubules, main collecting duct, main excretory tube, and excretory bladder; in the tail - the capillaries with filtering complex, collecting tubes, main caudal duct, and furcal duct. Differences in the morphology of the excretory system of cercaria and daughter sporocyst are discussed: they relate to the lack of a main collecting tube and excretory bladder, and to the presence of separate excretory pores in the sporocyst. The ultrastructure of the filtering complex of sporocysts differs in the more numerous cilia and the presence of internal leptotriches. The other difference is the presence of long cytoplasmic projections that are in contact with adjacent cells in the distal part of the sporocyst capillary cell.
This review presents the results of studies on the freshwater snail-trematode associations, carried out in Europe since the beginning of the 20th century. The great number of synonyms of snail and trematode specific names has made the cooperation between malacologists and parasitologists difficult. Here we provide a survey of larval trematodes (cercariae) based on Central, North and Western European literature. Whenever possible, the valid species name is provided, accompanied by synonyms, or just provisional names are used making up the largest part of the list. Trematodes with a definitely unclear systematic status are indicated as species incertae sedis. These include mostly trematodes which were attributed obviously erroneous names with poor or no description of their morphology. Although the provisional names inflate the number of trematode species and there are still many identification problems, we believe that this is another step in assessing the trematode species composition in Europe which will help not only parasitologists but also malacologists and contribute to their closer cooperation.
The life-cycle stages of a new species of Uvulifer, U. iruvettiensis sp. nov. infecting the white-throated kingfisher Halcyon smyrnensis (Linnaeus) in Kerala, India are described. The new species is described in detail, and compared with its related species. The furcocercous cercariae emerged from the planorbid snail Indoplanorbis exustus (Deshayes). Metacercariae were found below the skin of tadpoles of Euphlyctis cyanophlyctis (Schneider). H. smyrnensis became infected when fed on tadpoles containing infective metacercariae. Pre-patent period is 10 days. Successive stages in the development of metacercariae and adults were followed in the laboratory.
The organisation of the neuromuscular system in cercariae, metacercariae and adult Opisthorchis felineus was studied. The patterns of nerves immunoreactive (IR) to antibodies towards serotonin (5-HT) and FMRFamide are described in relation to the musculature, stained with TRITC-conjugated phalloidin. The general organisation of the musculature in the body wall, suckers, pharynx, intestine and sphincter of the excretory pore remains the same from the larval stages to the adult worms. However, the diameter of the individual muscle fibres increases distinctly in the adult worms. The general pattern of 5-HT IR fibres in cercariae, metacercariae and adult O. felineus remains the same. Despite the large increase in body size, the number of 5-HT IR neurones remains almost the same in the cercariae and metacercariae and only a modest increase in number of neurones was observed in the adult worms. Thus the proportion of 5-HT IR neurones/body mass is greatest in the actively moving cercariae. Anti-FMRFamide stains the nervous system strongly.
The variability and diagnostic usefulness of morphological features of metacercariae of two Diplostomum species, D. spathaceum and D. mergi, were studied on 104 and 85 specimens, respectively. Metacercariae were experimentally raised from cercariae in carp fry, Cyprinus carpio. Fourteen morphometric features and eight indices have been analysed. While no single variable could provide a classification with a reasonably small percentage of error, D. spathaceum and D. mergi have been separated with a misclassification rate less than 1% by applying a logistic regression model based on six significant variables (five metrical features and one index).
The life cycle of Acanthostomum burminis (Bhalerao, 1926) Bhalerao, 1936 (Trematoda, Acanthostomidae) infecting Xenochrophis piscator has been elucidated. Life cycle stages were successfully reared in the laboratory. The life cycle, from cercaria to adult, took more than two months to complete. Pleurolophocercous cercariae were found in the thiarid snail Thiara tuberculata. Metacercariae occurred in fin rays of several species of freshwater fishes. Snakes became infected when fed on fishes containing metacercariae. The prepatent period is 28 days. Successive stages in the development of metacercariae and adults were followed.
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