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Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum

100%

Krystynowicz A., Koziolkiewicz M., Wiktorowska-Jezierska A., Bielecki S., Klemenska E., Masny A., Plucienniczak A.

Acta Biochimica Polonica

2005

tom 52

nr 3

Acetobacter xylinum strains are known as very efficient producers of bacterial cellulose which, due to its unique properties, has great application potential. One of the most important problems faced during cellulose synthesis by these bacteria is generation of cellulose non-producing cells, which can appear under submerged culture conditions. The reasons of this remain unknow. These studies have been undertaken to compare at the molecular level wild-type, cellulose producing (Cel+) A. xylinum strains with Cel- forms of cellulose-negative phenotype. Comparison of protein profiles of both forms of A. xylinum by 2D electrophoresis allowed for the isolation of proteins which were produced exclusively by either Cel+ or Cel- cells. Sequences of peptides derived from these proteins were aligned with those of proteins deposited in databases. This analysis revealed that Cel- cells lacked two enzymes: phosphoglucomutase and glucose-1-phosphate uridylyltransferase, which generates UDP-glucose being the substrate for cellulose synthase. DNA was analyzed by ligation-mediated PCR carried out at low denaturation temperature (PCR-MP). Two DNA fragments of different thermal stability (218 and 217 bp) were obtained from the DNA of Cel+ and Cel- forms, respectively. The only difference between these Cel- and Cel+ DNA fragments is deletion of one T residue. Alignment of those two sequences with those deposited in the GenBank database revealed that similar fragments are present in the genomes of some bacterial cellulose producers and are located downstream from open reading frames (ORF) encoding phosphoglucomutase. The meaning of this observation is discussed.

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TSD1 (TUMORUS SHOOT DEVELOPMENT1) and TSD2 genes related with cell wall function are involved in somatic embryogenesis induced in vitro in Arabidopsis

84%

Kudelko K., Grzybkowska D., Gaj M. D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 3

Structure, function and metabolism of plant cell wall

67%

Kaczkowski J.

Acta Physiologiae Plantarum

2003

tom 25

nr 3

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Physiologiae Plantarum

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Bielecki S.

1 Gaj M. D.

1 Grzybkowska D.

1 Kaczkowski J.

1 Klemenska E.

1 Koziolkiewicz M.

1 Krystynowicz A.

1 Kudelko K.

1 Masny A.

1 Plucienniczak A.

1 Wiktorowska-Jezierska A.

1 2013

1 2005

1 2003

Wyniki wyszukiwania

Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum

TSD1 (TUMORUS SHOOT DEVELOPMENT1) and TSD2 genes related with cell wall function are involved in somatic embryogenesis induced in vitro in Arabidopsis

Structure, function and metabolism of plant cell wall