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This review presents recent data about cell wall involvement in plant embryogenesis. During plant development, the cell wall is subjected to precise regulation. During this process a bidirectional information exchange between the cell wall and the protoplast is observed. The cell wall also mediates in the cell-cell (apoplastic) and cell to cell (symplastic) information flow. Especially some products derived from the hydrolysis of specific cell wall compounds can act as short distance signal transduction molecules during the development. Oligosaccharins are a group of such products. Their activity and sources focused the researchers’ attention on the biochemical composition of the cell wall and the activity of some cell wall enzymes. The dramatic influence on the embryo body shape has also the cell wall synthesis machinery, including vesicular secretion pathways. Moreover, the interplay between the turgor pressure and counteracting cell walls and neighbouring cells (in higher organisms) creates the specific mechanical forces influencing the development of the whole plant. We conclude that discovering factors which can influence cell wall physiology and architecture is crucial for a better understanding of plant embryogenesis. In this review we summarize some recent experimental data reporting plant cell wall involvement in embryogenesis, putting special emphasis on somatic embryogenesis.
In this short communication we consider the extensibility properties of the cell wall. This is accomplished by a heuristically motivated equation for the expanding volume of the cell. The experimentally determined characteristic time t0 and temperature T0 are the only numbers required for evaluating the effective yielding coefficient Ф(t, T) in the respective time and temperature domains.
L-forms of S. epidermidis were induced at 35°C with the use of an L-form medium with penicillin. The aim of this study was to evaluate the frequency of L-form induction and demonstrate whether the origin of the clinical strains affects the frequency of L-forms induction, as well as to study whether the time of action of the antibiotic has an influence on frequency of L-form induction.
The epidermis of Helianthus hypocotyl can be peeled off and, in the form of detached strips can be used as a model system to study the effect on cortical microtubule (cMTs) orientation of these factors, which are difficult to be manipulated in situ, such as apoplastic pH or applied stress. In the first step, however, the orientation and reorientation of cMTs in the epidermis in situ must be described. The cMTs under the epidermal wall in hypocotyl epidermis at different positions along the hypocotyl and on its opposite sides were studied by means of immunostaining, using epi-fluorescence microscopy. The angle λ that parallel array of cMTs makes with cell longitudinal axis was measured. The variation of λ in a population of cells was documented by λ-histogram (frequency of cells exhibiting a particular λą∆λ plotted against λ value). The histograms were of either transverse type (maximum at λ ~90o, denoted as type A) or oblique type (two maxima on both sides of the transverse direction, denoted as type B) in the apical part of the hypocotyl, and were either of B type or of longitudinal type (maximum at λ ~0o or 180o denoted as type C) in the basal part. The change from A or B to C basipetally may be considered as due to the developmental trend in cMT orientation. The occurrence of B above A in some hypocotyls in their apical part strengthens the hypothesis on the autonomous reorientation of cMTs. The intermingled occurrence of A and B reorientation in the upper part of hypocotyl is interpreted as a manifestation of a subtle control of cell growth in latitudinal direction. The majority of histograms were asymmetric showing predominance of cMT parallel arrays inclined as the middle part of the letter Z.
A comprehensive analysis has been made of the mechanical properties and density of 100 timber species. The correlation between the mechanical properties and wood density has been approximated by a power function type y = aρn. No functional relation has been found between the parameters describing mechanical properties of the cell wall and the wood density. The values of these parameters show great scatter of about ±50% relative to the mean value. An attempt has been made to identify other wood characteristics determining the mechanical properties of wood. The species characterised by extremely high values of the parameters describing mechanical properties of wood have been singled out. The greatest differentiation in the values has been noted for the tensile strength. Among the coniferous species of similar density the differences have reached 113% on average, while among the deciduous species – 143%, at the differences in density being only of 15%.
Listeria monocytogenes is a ubiquitous gram-positive, rod-shaped, widespread in nature, facultative intracellular human and animal pathogen that causes infections collectively termed listeriosis. L. monocytogenes EGD encodes a total of 133 surface proteins, the abundance of which, as well as the variety of anchoring systems, probably reflects the ability of this bacterium to survive in diverse environments and to interact with many kinds of eukaryotic cells. The group of surface proteins also includes proteins with murein hydrolase activity-autolysins. To date, five L. monocytogenes autolysins have been identified: p60, P45, Ami, MurA and Auto. These enzymes are involved in numerous cellular processes including cell growth, cell wall turnover, peptidoglycan maturation, cell division and separation, formation of flagella, sporulation, chemotaxis and biofilm formation, genetic competence, protein secretion, the lytic action of some antibiotics and pathogenicity. We have recently identified a putative sixth listerial peptidoglycan-degrading enzyme, which has surprisingly been identified as FlaA, a flagellar protein of L. monocytogenes.
Ethambutol (EMB), the first line drug in the treatment of tuberculosis, is an inhibitor of the biosynthesis of the cell wall compound - arabinogalactan. It was found that EMB at sub-inhibitory concentration increases the permeability of the M. vaccae cell wall, which was monitored by cell sensitization to erythromycin and rifampicin. The high permeability of the cell wall to hydrophobic compounds allows enhanced intracellular bioconversion of (β -sitosterol to 4-androsten-3,17-dione (AD) and l,4-androstadien-3,17-dione (ADD).
The β-endoglucanase is one of the enzymes taking part in the degradation of the cell wall structural polysaccharides. The use of two Triticale varieties differing in their resistance to the preharvest sprouting allowed the comparison of that enzyme activity and changes in the internal structure of the cell wall observed in the light and electron microscope. The most interesting observations seem to be the channels and even the holes in the walls of aleurone cells found mostly in the samples showing the elevated activities of β-endoglucanase. As those samples concern the grains of a lower resistance to pre-harvest sprouting, it might be suggested that the loosening of the wall structure may be one of the probable mechanisms in facilitating the enzyme, metabolite and water translocations through the grain tissues. Those changes can accelerate the water imput into the grain, the metabolic process and thus the increased susceptibility to sprouting.
Ściany komórkowe glonów Botrydium granulátum, szczep 11-1 (Botrydiaceae, Botrydiales) zwane są macierzystymi ścianami komórkowymi (CWM). Gromadzone są one w pożywce wzrostowej i wykazują intensywnie różowe zabarwienie. Podobne zabarwienie wykazują ściany komórkowe izolowane z homogenatu z rozdrobnionych komórek (CWH). Zabarwienie to jest spowodowane przez karotenoidy, takie jak echinenon, kantaksantyna, 3S,3’S-astaksantyna, ich produkty utlenienia, astacen i semiastacen, 3S,3’-fritschiellaksantyna, 2,3-didehydro-(6’R,3’R)-fritschiellaksantyna i (3R,3’R,6’R) - luteina. Udowodniono, że ściany komórkowe (CW) glonów należących do Botrydiaceae, z rzędu Botrydiales zawierają te same karotenoidy co karotenoidy izolowane z CW rzędu Chlorococcales. Ściany komórkowe glonów Botrydium granulátum, szczep 11-1 zawierają także biopolimer oporny na acetolizę.
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