Wyniki wyszukiwania

1

Evaluation of intracellular Ca2plus concentration by Fura 2 ratiometry in encystment-induced Colpoda cucullus

100%

Sogame Y., Matsuoka T.

Acta Protozoologica

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2013

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tom 52

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nr 1

2

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

100%

Ryszawy D., Podulka J., Tworzydlo A., Tomassy J., Jarmulski M., Madeja Z., Korohoda W.

Folia Biologica

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2019

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tom 67

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nr 4

3

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Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions

100%

Niedziela A., Rybczynski J. J.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 3

Two-dimensional gel electrophoresis was used to compare protein expression profiles between various stages of Gentiana kurroo Royle somatic embryos. Seven distinct stages (I–VII) were pointed out and measured from long-term embryogenic cell suspension. Isoelectric focusing was done in the pH intervals 3–10, and the second dimension was carried out with 13% SDS-polyacrylamide gel electrophoresis. Dependent on the stage from about 400 (stage IV) to more than 700 (stage II) protein spots were totally detected. The molecular weight of abundant proteins range from 12 to 70 kDa, however, majority of proteins were located between 20–49 kDa spots on the gels. The highest difference in the number of spots appeared in the case of globular embryo (stage I) and elongated cotyledonary stage (stage VII) with differences being about 130 spots. The relevance of embryogenic cell suspension choice for proteomic analysis as well as expediency of the increasing number of particular embryo stages is discussed.

4

Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

100%

Mistrzak P., Celejewska-Marciniak H., Szypula W. J., Olszowska O., Kiss A. K.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

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nr 1

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

5

The modified method for isolation and culture of highly homogenous Leydig cell population from rat testes

100%

Janecki A., Pawlowski T., Lukaszyk A., Kozak W.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

6

Cell suspension cultures of Lemna minor L.

100%

Stefaniak B., Wozny A., Budna I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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1999

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tom 41

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nr 1

7

Somatic embryogenesis of Gentiana genus. III. Characterization of three-year-old embryogenic suspensions of G.pannonica originated from various seedling explants

100%

Mikula A., Skierski J., Rybczynski J. J.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 3

8

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

100%

Maciejewska U., Skierski J. S., Szczerbakowa A.

Acta Physiologiae Plantarum

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1999

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tom 21

|

nr 1

The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.

9

Influence of some polyols on erythrocyte cytoskeleton proteins

100%

Nardid O. A., Dyubko T. S., Soloviova A. S., Zinchenko A. V.

Cellular and Molecular Biology Letters

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1998

|

tom 03

|

nr 2

10

Cryopreserved tissue engineered mucosa

84%

Spoerl G., Mai R., Klingner E., Gedrange T., Lauer G.

Folia Histochemica et Cytobiologica

|

2008

|

tom 46

|

nr 4

541-544

11

Differentiation of wheat cell suspension cultured in cold - impact of zearalenone and 24-epibrassinolide

84%

Janeczko A., Biesaga-Koscielniak J., Skoczowski A., Marcinska I.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2007

|

tom 523

12

Improvement of growth parameters of prune callus cultures destined to initiate cell suspensions

84%

Hanus-Fajerska E.

Acta Societatis Botanicorum Poloniae

|

2006

|

tom 75

|

nr 1

5-9

Callus was inducted on wounded leaf explants from shoot tips of a particular Prunus domestica 'Węgierka Zwykła' clone cultivated in vitro. The improvement of Sweet Common Prune stock callus tissue parameters has been approached by experiments on culture protocols. Either for the induction or maintenance of tissue modified Murashige and Skoog medium, supplemented with different auxins and cytokinins at varying concentrations, was used. The goal was to obtain the highiest possible proliferative capacity of friable tissue without any signs of cell redifferentiation for about 10 weeks. The choice of auxin was an important factor regulating the rate and kind of tissue growth, and for the examined prune clone auxin alone brought a relatively small proportion of cells into division, so advantageous was to combine it with oxygenated cytokinin. Friable tissue was obtained on media supplemented with dicamba or with picloram, but not with 2.4-D neither alone nor combinated with IBA.

13

Reversible and irreversible electroporation of cell suspensions flowing through a localized dc electric field

84%

Korohoda W., Grys M., Madeja Z.

Cellular and Molecular Biology Letters

|

2013

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tom 18

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nr 1

Experiments on reversible and irreversible cell electroporation were carried out with an experimental setup based on a standard apparatus for horizontal electrophoresis, a syringe pump with regulated cell suspension flow velocity and a dcEF power supply. Cells in suspension flowing through an orifice in a barrier inserted into the electrophoresis apparatus were exposed to defined localized dcEFs in the range of 0–1000 V/cm for a selected duration in the range 10–1000 ms. This method permitted the determination of the viability of irreversibly electroperforated cells. It also showed that the uptake by reversibly electroperforated cells of fluorescent dyes (calcein, carboxyfluorescein, Alexa Fluor 488 Phalloidin), which otherwise do not penetrate cell membranes, was dependent upon the dcEF strength and duration in any given single electrical field exposure. The method yields reproducible results, makes it easy to load large volumes of cell suspensions with membrane non-penetrating substances, and permits the elimination of irreversibly electroporated cells of diameter greater than desired. The results concur with and elaborate on those in earlier reports on cell electroporation in commercially available electroporators. They proved once more that the observed cell perforation does not depend upon the thermal effects of the electric current upon cells. In addition, the method eliminates many of the limitations of commercial electroporators and disposable electroporation chambers. It permits the optimization of conditions in which reversible and irreversible electroporation are separated. Over 90% of reversibly electroporated cells remain viable after one short (less than 400 ms) exposure to the localized dcEF. Experiments were conducted with the AT-2 cancer prostate cell line, human skin fibroblasts and human red blood cells, but they could be run with suspensions of any cell type. It is postulated that the described method could be useful for many purposes in biotechnology and biomedicine and could help optimize conditions for in vivo use of both reversible and irreversible electroporation.

14

Age related decline in functional T cell subsets inthe mouse

84%

Boersma W. J. A., Vissinga C. S.

Folia Histochemica et Cytobiologica

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1986

|

tom 24

|

nr 1

15

Factors influencing cucumber [Cucumis sativus L.] somatic embryogenesis II. The genotype with different morphogenetic response specifically influencing culture parameters

84%

Wroblewski T., Malepszy S., Dmytrzak K.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1998

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tom 46

|

nr 2

16

Effects of cell suspension and cell-free culture filtrate of Pseudomonas aeruginosa in the control of root rot-root knot disease complex of tomato [Lycopersicon esculentum Mill.]

84%

Siddiqui I. A.

Acta Agrobotanica

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2000

|

tom 53

|

nr 2

The plant growth-promoting rhizobacterium Pseudomonas aeruginosa strain IE-6 was tested for antagonistic activity towards Meloidogyne javanica, the root-knot nematode and soilbome root-infecting fungi viz., Macrophomina phaseolina. Fusarium solani and Rhizoctonia solani under laboratory and greenhouse conditions. Cell-free culture filtrate of the bacterium caused significant reduction in egg hatching of M. javanica and inhibited radial growth of fungi in vitro. Cell-free culture filtrate also caused lyses in mycelium of F. solani. Under greenhouse conditions, soil drenches with the aqueous celi suspension or cell-free culture resulted in a considerable reduction in nematode population densities in soil and subsequent root-knot development due to M.javanica. In addition to nematode control, rhizobacterium application also inhibited root-infection caused by soilborne root-infecting fungi with significant enhancement of growth of tomato seedlings.

17

Plant regeneration from cell suspensions in Gerbera jamesonii Bolus

84%

Kumar S., Kumar Kanwar J. K.

Journal of Fruit and Ornamental Plant Research

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2007

|

tom 15

18

Effect of detoxified aflatoxin B1 contaminated products on some in vivo and in vitro plant physiological systems

84%

Das C., Mishra H. N.

Acta Physiologiae Plantarum

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2001

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tom 23

|

nr 3

19

Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures

84%

Sachdeva M., Mann A. P. S., Batta S. K.

Acta Physiologiae Plantarum

|

2005

|

tom 27

|

nr 1

Sugarcane cell cultures were obtained from callus formed on explants derived from young expanding leaves of two early maturing sugarcane varieties viz “CoJ83” and “CoJ86”. The cell cultures were varied with different arginine concentrations in the culture medium. For each cultivar, sucrose content with 20 μM arginine in the culture medium decreased from 3 to 5 days and then increased to 10 days after subculturing. Higher concentration of arginine in the culture medium (60 μM) decreased the sucrose content at different days after subculturing and thus significantly stimulated sucrose mobilization. The activity of sucrose synthase and sucrose phosphate synthase reached maximum while the activity of acid and neutral invertase was minimal in the culture medium with 20 μM arginine. Thus arginine at low concentration (20 μM) enables the cells to accumulate the higher level of sucrose. The optimum level of amino acids can be utilized to regulate the in vivo activity of sucrose synthase, sucrose phosphate synthase and invertase to achieve maximum sucrose accumulation in sugarcane storage tissue.

20

The effect of copper, zinc and iron ions on induction of the reactive oxygen species production in tobacco by-2 cell suspension culture

84%

Zrobek-Sokolnik A., Gorecki R. J.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

Ograniczanie wyników

Evaluation of intracellular Ca2plus concentration by Fura 2 ratiometry in encystment-induced Colpoda cucullus

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions

Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

The modified method for isolation and culture of highly homogenous Leydig cell population from rat testes

Cell suspension cultures of Lemna minor L.

Somatic embryogenesis of Gentiana genus. III. Characterization of three-year-old embryogenic suspensions of G.pannonica originated from various seedling explants

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

Influence of some polyols on erythrocyte cytoskeleton proteins

Cryopreserved tissue engineered mucosa

Differentiation of wheat cell suspension cultured in cold - impact of zearalenone and 24-epibrassinolide

Improvement of growth parameters of prune callus cultures destined to initiate cell suspensions