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Cell electrophoresis - a method for cell separation and research into cell surface properties

100%

Korohoda W., Wilk A.

Cellular and Molecular Biology Letters

2008

tom 13

nr 2

312-326

In this paper, we discuss the application of various methods of cell electrophoresis in research into cell surface properties (analytical methods), and the separation of uniform cell subpopulations from cell mixtures (preparative methods). The emphasis is on the prospects of the development of simplified and versatile methodologies, i.e. microcapillary cell electrophoresis and horizontal cell electrophoresis under near-isopycnic conditions. New perspectives are considered on the use of analytical and preparative cell electrophoresis in research on cell differentiation, neoplastic transformation, cell-cell interactions and the biology of stem cells.

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'

84%

Wilk A., Urbanska K., Woolley D. E., Korohoda W.

Cellular and Molecular Biology Letters

2008

tom 13

nr 3

366-374

This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

2 Korohoda W.

2 Wilk A.

1 Urbanska K.

1 Woolley D. E.

2 2008

Wyniki wyszukiwania

Cell electrophoresis - a method for cell separation and research into cell surface properties

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'