Wyniki wyszukiwania

1

Relationship between the size of cell and the number of its ciliary rows in the ciliate Dileptus

100%

Drzewinska J., Golinska K.

Acta Protozoologica

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1987

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tom 26

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nr 1

2

Differential responses to neutral red uptake of mating type Iand mating type II cells in Paramecium primaurelia. I. Cell size variation

100%

Ramoino P., Delmonte Corrado M. U.

Acta Protozoologica

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1992

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tom 31

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nr 1

3

cAMP - a secondary messenger in higher plants. The effects of cAMP level on endoreduplication and cell size growth during root cell differentiation

100%

Aniol P., Godlewski M., Domanska A., Lbik-Nowak A., Bilecka A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Ecological strategy at cell size level to respond to stressed environments

84%

Wang H., Liu Y., Chen H.

Polish Journal of Ecology

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2016

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tom 64

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nr 2

The cell size variations in woody plants in various environments are not well known, and the underlying mechanism has not been fully analyzed. 2. The current study focused on the intraspecific and interspecific size variations in palisade cells occurring in 76 woody species along an elevation gradient (1800–4500 m a.s.l.) on Gongga Montain (1400–7552 m a.s.l.) in southwest China, which included tropical and subtropical genera of broad-leaved forest flora including Salix hyperba as the species occurring on all elevations. We hypothesized that cell size is regulated by alterations in width-length scaling (L-D ratio) to mediate a surface-to-volume ratio (S/V) most suitable for the prevailing environmental conditions. 3. It was observed that cell size co-varied similarly with environmental conditions at the conspecifics, congenerics, and interspecifics levels investigated. Cell sizes decreased among conspecifics, or increase among congenerics and interspecifics via negative or positive scaling of width-length ratio at the cellular level. However, this dichotomously differing tendency may be ecologically constrained by both a cost-increasing and benefit-diminishing mechanism of cell dimension with increasing elevation at the species level, implying a ‘middle way’ strategy for species to stressed environments. 4. The implications of the results for regional floristic evolution are discussed.

5

Comparative effects of new generation oxazaphosphorines on the size and viability of human acute myeloblastic leukemia cells

84%

Mazur L., Opydo-Chanek M., Wojcieszek K., Stojak M., Niemeyer U.

Folia Biologica

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2012

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tom 60

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nr 1-2

6

Reversible and irreversible electroporation of cell suspensions flowing through a localized dc electric field

84%

Korohoda W., Grys M., Madeja Z.

Cellular and Molecular Biology Letters

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2013

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tom 18

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nr 1

Experiments on reversible and irreversible cell electroporation were carried out with an experimental setup based on a standard apparatus for horizontal electrophoresis, a syringe pump with regulated cell suspension flow velocity and a dcEF power supply. Cells in suspension flowing through an orifice in a barrier inserted into the electrophoresis apparatus were exposed to defined localized dcEFs in the range of 0–1000 V/cm for a selected duration in the range 10–1000 ms. This method permitted the determination of the viability of irreversibly electroperforated cells. It also showed that the uptake by reversibly electroperforated cells of fluorescent dyes (calcein, carboxyfluorescein, Alexa Fluor 488 Phalloidin), which otherwise do not penetrate cell membranes, was dependent upon the dcEF strength and duration in any given single electrical field exposure. The method yields reproducible results, makes it easy to load large volumes of cell suspensions with membrane non-penetrating substances, and permits the elimination of irreversibly electroporated cells of diameter greater than desired. The results concur with and elaborate on those in earlier reports on cell electroporation in commercially available electroporators. They proved once more that the observed cell perforation does not depend upon the thermal effects of the electric current upon cells. In addition, the method eliminates many of the limitations of commercial electroporators and disposable electroporation chambers. It permits the optimization of conditions in which reversible and irreversible electroporation are separated. Over 90% of reversibly electroporated cells remain viable after one short (less than 400 ms) exposure to the localized dcEF. Experiments were conducted with the AT-2 cancer prostate cell line, human skin fibroblasts and human red blood cells, but they could be run with suspensions of any cell type. It is postulated that the described method could be useful for many purposes in biotechnology and biomedicine and could help optimize conditions for in vivo use of both reversible and irreversible electroporation.

7

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Estimation of diatom and dinoflagellate cell volumes from surface waters of the Northern Indian Ocean

84%

Chitari R. R., Anil A. C.

Oceanologia

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2017

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tom 59

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nr 3

Phytoplankton samples collected from the Northern Indian Ocean (Bay of Bengal, northern Arabian Sea, and Dona Paula Bay Goa, west coast of India), were utilized to quantify changes in cell size, cell volume and carbon per cell of diatoms and dinoflagellates. The dataset from the Bay of Bengal also provides inter- and intra-annual variations (April 2008 to March 2010). The variations in cell size and volume were large in regions influenced by the riverine influx or terrigenous inputs. An interregional comparison of commonly available forms (8 species) points out that cell volumes are highest in the North Atlantic and lowest in the Mediterranean. The information provided will be useful in estimation of carbon biomass and biogeochemical studies.

8

Effect of MRI tags: SPIO nanoparticles and 19F nanoemulsion on various populations of mouse mesenchymal stem cells

67%

Muhammad G., Jablonska A., Rose L., Walczak P., Janowski M.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr 2

Transplantation of mesenchymal stem cells (MSCs) has emerged as a promising strategy for the treatment of myriad human disorders, including several neurological diseases. Superparamagnetic iron oxide nanoparticles (SPION) and fluorine nanoemulsion (19F) are characterized by low toxicity and good sensitivity, and, as such, are among the most frequently used cell-labeling agents. However, to date, their impact across the various populations of MSCs has not been comprehensively investigated. Thus, the impact of MRI tags (independent variable) has been set as a primary endpoint. The various populations of mouse MSCs in which the effect of tag was investigated consisted of (1) tissue of cell origin: bone marrow vs. adipose tissue; (2) age of donor: young vs. old; (3) cell culture conditions: hypoxic vs. normal vs. normal + ascorbic acid (AA); (4) exposure to acidosis: yes vs. no. The impact of those populations has been also analyzed and considered as secondary endpoints. The experimental readouts (dependent variables) included: (1) cell viability; (2) cell size; (3) cell doubling time; colony formation; (5) efficiency of labeling; and (6) cell migration. We did not identify any impact of cell labeling for these investigated populations in any of the readouts. In addition, we found that the harsh microenvironment of injured tissue modeled by a culture of cells in a highly acidic environment has a profound effect on all readouts, and both age of donor and cell origin tissue also have a substantial influence on most of the readouts, while oxygen tension in the cell culture conditions has a smaller impact on MSCs. A detailed characterization of the factors that influence the quality of MSCs is vital to the proper pursuit of preclinical and clinical studies.

9

Particle-associated planktonic naked amoebae in the Hudson Estuary: size-fraction related densities, cell sizes and estimated carbon content

67%

Anderson O. R.

Acta Protozoologica

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2011

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tom 50

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nr 1

10

Apoptosis, proliferation, and cell size in seasonal changes of body and organ weight in male bank voles Myodes glareolus

67%

Bonda-Ostaszewska E., Wlostowski T.

Acta Theriologica

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2015

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tom 60

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nr 3

11

Anatomical characteristics of hypocotyl of sugar beets different in sugar content

67%

Sliwinska E., Dziamski A., Steen P.

Journal of Applied Genetics

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1995

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tom 36

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nr 3

Six seedling hypocotyl anatomical characters of sugar beet diploid lines and triploid hybrids were measured. Root yield and sugar content of these lines and hybrids were evaluated in replicated field trials. Some of the studied hypocotyl characters: the diameter of the central core, the diameter of parenchymatic cells outside the central core and the width of xylem, correlated negatively with sugar content and positively with root yield. This suggests that these parameters can be used in preliminary selection of sugar-beet breeding material. Introducing such criteria into the breeding process could speed up the selection and reduce the number of expensive field trials.

12

Method of parenchyma cells parametrisation using fluorescence images obtained by confocal scanning laser microscope

67%

Zdunek A., Umeda M., Konstankiewicz K.

Electronic Journal of Polish Agricultural Universities. Series Agricultural Engineering

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2004

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tom 07

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nr 1

Fruits and vegetables are built mainly of thin walled parenchyma cells highly susceptible to mechanical damage. According to experimental investigation of relationship between cells geometrical parameters and mechanical properties of tissue a new efficient method of structure reconstruction and parametrisation is elaborated and presented in this paper. Special sample preparation and way of image taking by Confocal Scanning Laser Microscope with fluorescence mode allows obtaining images of any number of cells. Unique computer procedure based on morphological operators was created that allows analysing automatically any number of images with extracting geometrical parameters of each cell separately. The method was tested on potato and carrot tissue. Results show that the method is fast and gives proper cell reconstruction for both materials. In order to decrease error of incorrect reconstruction, possibility of manual correction was introduced to the procedure.

13

Influence of turgor and cell size on the cracking of potato tissue

67%

Konstankiewicz K., Zdunek A.

International Agrophysics

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2001

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tom 15

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nr 1

14

Cell enlargement in suspension culture of carrot [Daucus carota L.Cv.Karo]. Preliminary studies

67%

Golczyk H.

Acta Biologica Cracoviensia. Series Botanica

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1998

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tom 40

Cytological studies of Daucus carota L. (cv. Karo) in suspension culture were made. Cells growing on medium without ammonium were larger and more vacuolated than were cells grown on the standard medium. Their sizes (cell area) ranged widely from 200 µrm2 to 40000 µm2. They also showed a very low level of aggregation (1%) as compared to an 81% frequency of aggregates in the standard suspension. Suspension deficient in ammonium had a .small percentage of 2-9-nucleated cells. Such multlnucleate cells were not observed in suspension with both nitrate and ammonium used as nitrogen source.

Ograniczanie wyników

Relationship between the size of cell and the number of its ciliary rows in the ciliate Dileptus

Differential responses to neutral red uptake of mating type Iand mating type II cells in Paramecium primaurelia. I. Cell size variation

cAMP - a secondary messenger in higher plants. The effects of cAMP level on endoreduplication and cell size growth during root cell differentiation

Ecological strategy at cell size level to respond to stressed environments

Comparative effects of new generation oxazaphosphorines on the size and viability of human acute myeloblastic leukemia cells

Reversible and irreversible electroporation of cell suspensions flowing through a localized dc electric field

Estimation of diatom and dinoflagellate cell volumes from surface waters of the Northern Indian Ocean

Effect of MRI tags: SPIO nanoparticles and 19F nanoemulsion on various populations of mouse mesenchymal stem cells

Particle-associated planktonic naked amoebae in the Hudson Estuary: size-fraction related densities, cell sizes and estimated carbon content

Apoptosis, proliferation, and cell size in seasonal changes of body and organ weight in male bank voles Myodes glareolus

Anatomical characteristics of hypocotyl of sugar beets different in sugar content

Method of parenchyma cells parametrisation using fluorescence images obtained by confocal scanning laser microscope

Influence of turgor and cell size on the cracking of potato tissue

Cell enlargement in suspension culture of carrot [Daucus carota L.Cv.Karo]. Preliminary studies