Wyniki wyszukiwania

1

Can GFP expression affect cell physiology?

100%

Zakrzewski P., Lenartowska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Changes in cell physiology at the stage of dehydration in a cryopreservation protocol

88%

Domzalska L., Kedracka-Krok S., Mikula A., Rybczynski J. J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

3

The effect of antisense oligonucleotide treatment of plasma membrane Ca2plus-ATPase in PC12 cells

75%

Szemraj J., Kawecka I., Bartkowiak J., Zylinska L.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 3

Plasma membrane Ca2+-ATPase (PMCA), encoded by four separate genes, constitutes a high affinity system extruding Ca2+ outside the cell. The nerve growth factor-treated PC12 cell line possesses all four main PMCA isoforms. To evaluate the potential role of PMCA isoforms in the differentiation process, we transiently suppressed the expression of PMCA2 and 3 using the antisense oligonucleotides. In the transfected PC12 cells, we observed morphological changes, slowed neurite extension and diminished survival of the cells. The apparent transport activity and affinity of the calcium pump to Ca2+ were lower in the cells with suppressed PMCA2 and 3 isoforms than in the control cells. Moreover, in the transfected PC12 plasma membranes, the calcium pump was insensitive to stimulation by calmodulin. These findings suggest that PMCA2 and 3 isoforms may be involved in developmental and differentiation processes.

4

Modulation of the voltage-dependent anion-selective channel by cytoplasmic proteins from wild type and the channel depleted cells of Saccharomyces cerevisiae

63%

Kmita H., Budzinska M., Stobienia O.

Acta Biochimica Polonica

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2003

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tom 50

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nr 2

It is well known that effective exchange of metabolites between mitochondria and the cytoplasm is essential for cell physiology. The key step of the exchange is transport across the mitochondrial outer membrane, which is supported by the voltage-dependent anion-selective channel (VDAC). Therefore, it is clear that the permeability of VDAC must be regulated to adjust its activity to the actual cell needs. VDAC-modulating activities, often referred to as the VDAC modulator, were identified in the intermembrane space of different organism mitochondria but the responsible protein(s) has not been identified as yet. Because the VDAC modulator was reported to act on VDAC of intact mitochondria when added to the cytoplasmic side it has been speculated that a similar modulating activity might be present in the cytoplasm. To check the speculation we used mitochondria of the yeast Saccharomyces cerevisiae as they constitute a perfect model to study VDAC modulation. The mitochondria contain only a single isoform of VDAC and it is possible to obtain viable mutants devoid of the channel (Δpor1). Moreover, we have recently characterised a VDAC-modulating activity located in the intermembrane space of wild type and Δpor1 S. cerevisiae mitochondria. Here, we report that the cytoplasm of wild type and Δpor1 cells of S. cerevisiae contains a VDAC-modulating activity as measured in a reconstituted system and with intact mitochondria. Since quantitative differences were observed between the modulating fractions isolated from wild type and Δpor1 cells when they were studied with intact wild type mitochondria as well as by protein electrophoresis it might be concluded that VDAC may influence the properties of the involved cytoplasmic proteins. Moreover, the VDAC-modulating activity in the cytoplasm differs distinctly from that reported for the mitochondrial intermembranen space. Nevertheless, both these activities may contribute efficiently to VDAC regulation. Thus, the identification of the proteins is very important.

5

Zachowanie się komórek jąder nadwzrokowego i przykomorowego podwzgórza i komórek ependymalnych narządu podspoidłowego szczura po podaniu wyciągu z tylnego płata przysadki

51%

Czechowicz K., Skowerska M., Siekierska E., Kotula A.

Acta Biologica Silesiana

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1990

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tom 15

6

DNA methylation in states of cell physiology and pathology

51%

Sulewska A., Niklinska W., Kozlowski M., Minarowski L., Naumnik W., Niklinski J., Dabrowska K., Chyczewski L.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 3

7

Stan fizjologiczny unieruchomionych komorek drozdzy w czasie fermentacji etanolowej

45%

Paslawska M.

Żywność Nauka Technologia Jakość. Suplement

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1999

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tom 06

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nr 3

76-81

W pracy określano wpływ stężenia alginianu glinu i alginianu wapnia oraz stężenia inokulum na stan fizjologiczny drożdży Saccharomyces cerevisiae V30 w czasie okresowej fermentacji etanolowej. Stwierdzono, że stan fizjologiczny komórek zależy od wszystkich trzech badanych parametrów.

Ograniczanie wyników

Can GFP expression affect cell physiology?

Changes in cell physiology at the stage of dehydration in a cryopreservation protocol

The effect of antisense oligonucleotide treatment of plasma membrane Ca2plus-ATPase in PC12 cells

Modulation of the voltage-dependent anion-selective channel by cytoplasmic proteins from wild type and the channel depleted cells of Saccharomyces cerevisiae

Zachowanie się komórek jąder nadwzrokowego i przykomorowego podwzgórza i komórek ependymalnych narządu podspoidłowego szczura po podaniu wyciągu z tylnego płata przysadki

DNA methylation in states of cell physiology and pathology

Stan fizjologiczny unieruchomionych komorek drozdzy w czasie fermentacji etanolowej