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1

Cell electrophoresis - a method for cell separation and research into cell surface properties

100%
Korohoda W., Wilk A.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 2
312-326
In this paper, we discuss the application of various methods of cell electrophoresis in research into cell surface properties (analytical methods), and the separation of uniform cell subpopulations from cell mixtures (preparative methods). The emphasis is on the prospects of the development of simplified and versatile methodologies, i.e. microcapillary cell electrophoresis and horizontal cell electrophoresis under near-isopycnic conditions. New perspectives are considered on the use of analytical and preparative cell electrophoresis in research on cell differentiation, neoplastic transformation, cell-cell interactions and the biology of stem cells.
2

A new method for the preperative and analytical electrophoresis of cells

100%
Wilk A., Roskowicz K., Korohoda W.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 4
In this paper, a new method is described for the horizontal electrophoresis of cells on a density cushion under near-isopycnic conditions. When cell sedimentation is minimized, the electrophoresis of red blood cells (RBC) used as model cells within an anti-convective porous matrix (with pores over 300 μm in diameter) was capable of separating a mixture of human and chicken RBC according to their electrophoretic mobilities. Samples taken from the separated RBC bands show over 90% purity for each species. The simultaneous electrophoresis of several RBC samples carried out under identical conditions permitted the use of comparative data based on the electrophoretic mobility of cells which differ in their surface properties. We believe that this relatively simple system, in which cell sedimentation and convection are minimized, has the potential to be modified and adapted for the separation of other cell types/organelles.
3

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'

84%
Wilk A., Urbanska K., Woolley D. E., Korohoda W.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 3
366-374
This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.
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