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1

Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell IL-6 and IL-8 secretion and E-selectin and VCAM-1 expression

100%
Weglarz L., Dzierzewicz Z., Skop B., Orchel A., Parfiniewicz B., Wisniowska B., Swiatkowska L., Wilczok T.
Cellular and Molecular Biology Letters
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2003
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tom 08
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nr 4
The aim of this study was to determine whether Desulfovibrio desulfuricans-derived LPS stimulate the release of IL-6 and IL-8 from ECs and the expression of their adhesion molecules at the transcriptional level. Confluent monolayers of HUVEC were incubated in the absence or presence of 20 μg/ml and 60 μg/ml LPSs derived from the DdT and DdA bacterial strains. Also, the simultaneous stimulation of cells with LPSs and IL-1β was evaluated. The levels of cytokines released were measured using ELISA. LPS-activated HUVEC increased the secretion of both IL-6 and IL-8, which was not LPS dose dependent. The expression of E-selectin and VCAM-1 was assessed by TR-PCR. The transcripts were detectable at all the concentrations (20, 40, 60 μg/ml) of LPSs used. These results suggest that D. desulfuricans LPS may activate immune functions in endothelial cells and influence the inflammatory response during bacteremia caused by these bacteria.
2

Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches

100%
Nowak M., Madej J. A., Dziegiel P.
Folia Histochemica et Cytobiologica
|
2007
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tom 45
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nr 3
3

The role of cell adhesion molecule in cancer progression and its application in cancer therapy

100%
Okegawa T., Pong R. C., Li Y., Hsieh J. T.
Acta Biochimica Polonica
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2004
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tom 51
|
nr 2
Multiple and diverse cell adhesion molecules take part in intercellular and cell-extracellular matrix interactions of cancer. Cancer progression is a multi-step process in which some adhesion molecules play a pivotal role in the development of recurrent, invasive, and distant metastasis. A growing body of evidence indicates that alterations in the adhesion properties of neoplastic cells play a pivotal role in the development and progression of cancer. Loss of intercellular adhesion and the des­quamation of cells from the underlying lamina propria allows malignant cells to es­cape from their site of origin, degrade the extracellular matrix, acquire a more motile and invasion phenotype, and finally, invade and metastasize. In addition to partici­pating in tumor invasiveness and metastasis, adhesion molecules regulate or signifi­cantly contribute to a variety of functions including signal transduction, cell growth, differentiation, site-specific gene expression, morphogenesis, immunologic function, cell motility, wound healing, and inflammation. Cell adhesion molecule (CAM), a di­verse system of transmembrane glycoproteins has been identified that mediates the cell–cell and cell–extracellular matrix adhesion and also serves as the receptor for different kinds of virus. We summarize recent progress regarding the role of CAM, particularly, immunoglobulin-CAMs and cadherins in the progression of cancer and discuss the potential application of CAMs in the development of cancer therapy mainly on urogenital cancer.
4

P-selectin mediates adhesion of leucocytes, platelets, and cancer cells in inflammation, thrombosis, and cancer growth and metastasis

100%
Chen M., Geng J. G.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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tom 54
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nr 2
5

The interaction between L1-type proteins and ankyrins - a master switch for L1-type CAM functions

80%
Hortsch M., Nagaraj K., Godenschwege T. A.
Cellular and Molecular Biology Letters
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2009
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tom 14
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nr 1
57-69
L1-type cell adhesion molecules (CAMs) are important mediators of neural differentiation, including axonal outgrowth and pathfinding and also of synapse formation and maintenance. In addition, their interactions with cytoskeletal components are highly conserved and regulated. How these different aspects of CAM functionality relate to each other is not well understood. Based on results from our and other laboratories we propose that ankyrin-binding to L1-type CAMs provides a master switch. The interaction with ankyrins directs L1-type adhesive proteins into different functional contexts, either ankyrin-independent functions, such as neurite outgrowth and axonal pathfinding or into ankyrin-dependent functions, such as L1’s role at axon initial segments (AIS), paranodal regions, synapses and in dendrites.
6

Cloning of the human activated leukocyte cell adhesion molecule promoter and identification of its tissue-independent transcriptional activation by Sp1

80%
Tan F., Mbunkui F., Ofori-Acquah S. F.
Cellular and Molecular Biology Letters
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2012
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tom 17
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nr 4
Activated leukocyte cell adhesion molecule (ALCAM) belongs to the immunoglobulin cell adhesion molecule super family. ALCAM is implicated in tumor progression, inflammation, and the differentiation of hematopoietic stem cells. Hitherto, the identity of regulatory DNA elements and cognate transcription factors responsible for ALCAM gene expression remained unknown. In this report, the human ALCAM promoter was cloned and its transcriptional mechanisms elucidated. The promoter is TATA-less and contains multiple GC-boxes. A proximal 650-bp promoter fragment conferred tissue-independent activation, whereas two contiguous regions upstream of this region negatively influenced promoter activity in a tissue-specific manner. The positive regulatory promoter region was mapped to a core 50 base pair sequence containing a conical Sp1 element. Mutation analysis revealed that this element alone or in tandem with elements immediately upstream was required for maximal promoter activity. Chromatin analysis revealed that Sp1 binds exclusively to the canonical binding sequence in vivo, but not to DNA sequence immediately upstream. Finally, we showed that over-expression of Sp1 significantly increased the basal promoter activity. Thus, Sp1 activated the ALCAM promoter in most cells. These findings have important ramifications for unraveling the roles of ALCAM in inflammation and tumorigenesis.
7
Content available remote

Effects of ovariectomy in prepubertal goats

80%
Dessauge F., Finot L., Wiart S., Aubry J. M., Ellis S. E.
Journal of Physiology and Pharmacology. Supplement
|
2009
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tom 60
|
nr 3
127-133
8
Content available remote

Trans fatty acids induce a proinflammatory response in endothelial cells through ROS-dependent nuclear factor-kappaB activation

80%
Bryk D., Zapolska-Downar D., Malecki M., Hajdukiewicz K., Sitkiewicz D.
Journal of Physiology and Pharmacology
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2011
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tom 62
|
nr 2
It has been shown that increased intake of trans fatty acids (TFAs) is associated with a higher risk of cardiovascular disease. In this study, we have investigated the effects of linoelaidic (LA) and elaidic (EA) acids on the proinflammatory response in endothelial cells, a key step in vascular disease. Human aortic endothelial cells (HAECs) were treated with different concentrations (100 µmol/l in most experiments) of LA or EA for different periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined by flow cytometry and real time RT-PCR, respectively. Adhesion of leukocytes to TFA-treated HAECs was evaluated by an adhesion assay. Activation of nuclear factor-B (NF-B) was evaluated by measuring NF-B p65 phosphorylation using flow cytometry. ROS production was determined by the reduction of fluorescent 2',7'-dichlorofluorescein diacetate (DCFH-DA). LA treatment significantly increased protein and mRNA levels of ICAM-1 and VCAM-1, leukocyte adhesion to HAECs, phosphorylation of NF-B and ROS generation. Similar effects were achieved for cells incubated with EA. Experiments with HAECs pretreated with pyrrolidine dithiocarbamate, an inhibitor of NF-B, revealed that both LA and EA-mediated induction of ICAM-1 and VCAM-1 is mainly regulated by NF-B. The ROS production induced by both of the studied acids was inhibited in the presence of diphenyleneiodonium (DPI), a NADPH oxidase inhibitor, suggesting ROS production through the activation of NADPH oxidase. Furthermore, LA or EA-induced ICAM-1 and VCAM-1 expression, activation of NF-B and adhesion of leukocytes to HAECs were abolished in the presence of DPI. Conclusion: TFAs present in our diet have a direct proinflammatory effect, which promotes leukocyte adhesion to the endothelium through ROS-dependent NF-B activation.
9

A novel isoform of human lymphoid enhancer-binding factor-1 [LEF-1] gene transcript encodes a protein devoid of HMG domain and nuclear localization signal

80%
Kobielak A., Kobielak K., Trzeciak W. H.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
Lymphoid enhancer-binding factor-1 (LEF-1), a member of the high mobility group (HMG) family of proteins, regulates expression of T-cell receptor-a gene and is one of the key regulatory molecules in the epithelial-mesenchymal interactions during embryonic de­velopment. Among others, LEF-1 regulates expression of cytokeratin genes involved in formation of hair follicles and the gene encoding the cell-adhesion molecule E-cadherin. Transcription factor LEF-1, which acts as a dimer, binds ß-catenin and is involved in signal transduction by the wnt pathway. We have cloned and sequenced a novel isoform of human LEF-1 gene transcript. This isoform encodes a truncated protein devoid of HMG domain and nuclear localization sig­nal but retaining ß-catenin binding domain. This isoform might either act in a domi­nant-negative manner by interfering with native LEF-1, or might bind ß-catenin in the cytosol, which would result in attenuation of the signals transmitted by theLEF-ß-catenin pathway.
10

Nuclear factor-kappaB: a key regulator in health and disease of lungs

71%
Batra S., Balamayooran G., Sahoo M. K.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 5
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