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Castration in male pigs: techniques and animal welfare issues

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Castration in male pigs is usually performed during the first weeks of life without prior anesthesia. This technique, however, is known to induce acute pain and stress and will therefore not be tolerated any longer by animal welfare organizations. Practical and animal-friendly alternatives to surgical castration are the production of entire male pigs, semen sexing or immunological castration. Fattening boars has the benefits of better feed efficiency, higher lean meat yield and increased animal welfare due to no pain and stress of castration. The most important disadvantage in raising entire male pigs is the incidence of boar taint ranging between 10 and 75%. To identify tainted carcasses an accurate and rapid on-line method for detection of odorous compounds is absolutely necessary. Sperm sexing through flow cytometry is the only commercially available method at the moment but speed of separation is too low for practical application. Active immunization of boars against gonadotropin-releasing-hormone (GnRH) at the end of the fattening period results in a significant reduction of testicular weight and androstenone production while the benefits of daily growth gain, meat quality as well as welfare remain the same as in entire males. In the present review more detailed information is given about the various techniques, especially the practical application of immunocastration on a large scale base.
The present study was carried out on sexually mature boars. All the animals were injected with fast blue into right testis and then divided into four groups (G1- control animals, G2 - hemicastrated, G3 - castrated, and G4 - castrated and injected with testosterone). After 3 weeks, G1 pigs were transcardially perfused. In G2 pigs right testes, whereas in G3 and G4 animals both testes were removed. G4 pigs were injected with testosterone. After 2 weeks, the pigs were transcardially perfused and then their caudal mesenteric ganglia (CaMG) and anterior pelvic ganglia (APG) were collected. The ganglia were cut into 12 µm-thick cryostat sections. Sections were stained using antisera against TH or DßH, VACHT or CHAT, NPY, VIP and GAL, and androgen receptor (AR). Immunohistochemical staining of CaMG-sections revealed that approximately 74% of FB-positive (FB⁺) neurons contained immunoreactivity to TH or DßH, whereas 4% of FB⁺ cells were VACHT-positive. Among FB⁺/DßH⁺ neurons, 72% contained NPY and 2% stained for GAL. All FB⁺/VACHT⁺ neurons were also VIP⁺. 62% of FB⁺ somata were NPY⁺, whereas 6% stained for VIP. In all experimental animals, numbers of FB⁺perikarya immunoreactive to TH (approx. 30%) and DßH (approx. 50%) were smaller than those found in G1 animals, whereas numbers of neurons displaying immunoreactivity to other substances studied were higher. The most significant increases regarded those expressing GAL (approx. 30%) and VIP (approx. 20%) whereas less distinct changes dealt with NPY⁺ and VAChT⁺ or ChAT⁺ neurones. In APG, 60% of FB⁺ neurons contained immunoreactivity to TH or DßH, whereas 12% of FB⁺ cells were VACHT-positive. Among FB⁺/DßH⁺ neurons, 55% contained NPY and 3% stained for GAL. All FB⁺/VACHT⁺ neurons were also VIP⁺. 46% of FB⁺ somata were NPY-IR, whereas 19% stained for VIP. In all experimental pigs, the immunohistochemical properties of the APG FB⁺ neurones were similar to those found in relation to CaMG-perikarya. Neurones of both studied ganglia were surrounded by dense networks of VACHT-positive nerve fibres. The most apparent changes in the immunohistochemical features of the FB⁺ neurons evoked by bilateral castration were observed in G3 pigs; whereas changes found in G4 were very similar to those observed in G2 animals.
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