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The influence of the time of distillation on the content and composition of essential oil isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation was investigated. The GC-MS data showed that the essential oil was rich in carvacrol (30.90–46.16%) and its biogenetic precursors γ-terpinene (5.72–9.09%) and p-cymene (4.51–13.46%). Other constituents identified in significant amounts were β-caryophyllene, carvacrol methyl ether and (-)-β-bisabolene. On the basis of the obtained data it was proved that time of distillation had no effect on essential oil content. Moreover, there was no significant effect of the distillation time on the content of essential oil constituents found in the experiment.
The occurrence of multidrug-resistant Staphylococcus aureus is an important causative agent of mastitis in cattle and of foodborne diseases. It is a worldwide concern, making it essential to develop alternative treatments to fight against the bacteria. Thus, the aim of this study is to determine the ability of carvacrol to inhibit the growth of S. aureus isolated from bulk tank milk in Turkey’s Burdur Province. All strains (n = 31) were used to investigate the antimicrobial activity of carvacrol, including the methicillin-resistant S. aureus and strains from the American Type Culture Collection and England’s National Collection of Type Cultures. The minimum inhibitory concentration (MIC) values were determined via a microdilution method, and the antimicrobial susceptibility profiles via a disk diffusion method. Antibiotic resistance was detected in 20 strains (64.5%). Multidrug resistance was observed in 8 strains (25.8%). Carvacrol exhibited strong antimicrobial activity, with MIC value at 0.058-0.234 mg/ml, in the microdilution method. Inhibition zones of carvacrol were in the range of 19 to 45 mm. The results of this study emphasize the promising role of carvacrol among new antibacterial agents that can combat S. aureus strains.
Two experiments were conducted to examine effects of a commercial phytogenic feed additive (PFA), containing inulin, an essential oil mix (carvacrol, thymol), and chest nut meal (polyphenols) on nutrient digestibility, protein utilization, and lysine efficiency in piglets. Each experiment utilized sixteen piglets (male castrated, 8 wk) and four experimental groups. Experiment 1 studied graded levels of the PFA (0, 0.05, 0.1, 0.15%) in lysine limited diets (wheat, barley, soyabean meal, fish meal). Experiment 2 examined 0.1% of the PFA and two dietary levels of lysine. The results indicated that apparent nutrient digestibility, protein utilization, and lysine efficiency were not significantly affected by the PFA (P>0.05). Observed daily lysine requirements (7.3, 9.9 and 13.1 g for 76, 100, 124 g daily protein deposition) in growing barrows (20-30 kg body weight) were in line with recommendations and contribute to the limited database for modelling of lysine requirements in piglets.
Thymus broussonettii, a Moroccan endemic plant, exists in two chemotypes. The aim of our study is to compare the cytotoxic activity of their essential oils and major products as well as their effect on cell cycle and apoptosis induction. The chemical composition analysis of essential oils by GC-SM revealed that the lasts are rich and diverse and the major products of the chemotypes TbA and TbE are carvacrol and thymol, respectively. The in vitro cytotoxic effect study against five tumor cell lines shows that TbA essential oil, rich in carvacrol, has an important cytotoxic effect, higher than that of TbE, rich in thymol. This result is confirmed by comparing cytotoxic effect of carvacrol and thymol. Furthermore, TbA EO /carvacrol and TbE EO /thymol induce cell cycle arrest at S and G0/G1 phases, respectively. On the other hand, carvacrol, most cytotoxic in vitro, was studied for its effect on solid tumor in vivo and apoptosis-induction. Our results show that carvacrol, administred by gavage, has an important effect on solid tumor and induce apoptosis in P815 tumor cell line.
Introduction: In Iranian traditional medicine, Zataria multiflora Boiss (Lamiaceae family) is reputed due to its antiseptic effects. Objective: The purpose of this study was to evaluate the antibacterial and biofilm killing effects of Z. multiflora essential oil and main components against Pseudomonas aeruginosa. Methods: The main components of essential oil were identified by gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS). The antibacterial properties of Z. multiflora oil and main components were determined by assessing the MIC and MBC values, and their inhibition percent of biofilm killing effects were determined by the evaluation of optical density. The role of each main component in these activities was determined according to the chemical profiles of essential oil. Results: Thymol (38.7%), carvacrol (30.6%), and p-cymene (8.3%) were main components of twenty five components of essential oil. Carvacrol had the higher role in antibacterial activity against P. aeruginosa, followed by thymol. P-cymene enhanced the antibacterial activities of thymol and carvacrol against P. aeruginosa. Carvacrol showed the weak role in biofilm killing effect. In spite of the low antibacterial activity of p-cymene against P. aeruginosa, it can enhance the antibacterial activity of thymol or carvacrol. Conclusion: Z. multiflora essential oil can be used for the management of P. aeruginosa infections. Determining the precise role of each components needs investigating in their behavior in different media.
Oliveria decumbens Vent (Umbelliferae) is a shrub commonly found in the South-East of Iran. Its aerial section is extensively used in herbal medicine. The Disk Diffusion Test and Microbroth Dilution Assay were used to determine the antimicrobial activity of the essential oil from Oliveria decumbens Vent against Staphylococcus aureus. To detect synergy, vancomycin was added to Mueller-Hinton agar at sub-inhibitory concentrations and the inhibitory zones were recorded in millimeters. The main components of oil were thymol (22%), carvacrol (22%) and p-cymene (19%). The O. decumbens oil exhibited strong antistaphylococcal activity (18.0±0.86). Carvacrol was considerably more effective (29.8±1.5) than thymol (17.2±1.13) and p-cymene (0.0±0.0) against Staphylococcus aureus. The oil presented strong synergism with vancomycin (24.9±0.75 vs. 19.3±0.54, p<0.001). However, further studies are required to evaluate its in vivo efficacy.
The experiment was conducted in the years 2004-2005. The aim of the study was to determine the chemical composition of essential oils from garden thyme herb in the first year of cultivation. Essential oil was achieved in distillation with water vapor according to Polish Pharmacopoeia VI. Qualitative and quantitative composition of essential oils was analyzed using gas chromatography combined with mass spectroscopy technique (GC/MS). The analysis revealed the presence of 37 chemicals in essential oil made of thyme, including one unidentified. Thymol, a-terpinene, p-cymene and carvacrol were the main components of essential oil achieved from garden thyme cultivated in south­eastern Poland.
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