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The authors investigated carotenoids in selected body parts of pike before, during, and post-spawning period.
Essential nutritional components are quantified in the study in addition to major antioxidants, carotenoids, phenols and flavonoids. Their antioxidant activities were also analyzed using DPPH, ABTS and hydrogen peroxide radical scavenging activities and by determining their iron reducing power. Results indicated that the highest quantity of nutritional and antioxidant components was obtained in red variety (4.63 mg/g ascorbic acid, 10.32 mg/g total carotenoid content, 61.50 mg/g total flavonoid content, 310.27 mg/g total phenolic content) followed by orange, yellow and green varieties. Similar trend was observed while analyzing their antioxidant activities by different methods. In studying correlation between components and antioxidant activity, a strong correlation was obtained for ABTS radical scavenging activity with total phenolic content (R2 = 0.722) and total carotenoid contents (R2 = 0.709), while moderate correlation was observed for ABTS radical scavenging activity with total flavonoid contents (R2 = 0.517) and ascorbic acid (R2 = 0.673).
We evaluated the carotenoid profile and concentration (by HPLC) and the phytochemical content of two OFSP varieties (Umuspo 3 and Ex-Igbariam) planted at three distances (20 cm, 30 cm and 40 cm) and harvested in two different periods (12th and 16th weeks after planting) respectively. Carotene contents of the outer peel and inner flesh of the sweet potato varieties were also determined. The results showed wide variation in the carotenoid and phytochemical content among the varieties at different planting spaces and harvest periods. Umuspo 3 planted at 20 cm, 30 cm and 40 cm had significantly greater carotenoid concentration than Ex-Igbariam variety. The predominant carotenoid was β-carotene with highest concentration obtained from 40 cm planting distance (92.82µg/g) and 30 cm (80.97µg/g) for Umuspo 3. Ex-Igbariam at 30 cm planting distance contained 2.51µg/g β-carotene when harvested after 16th weeks. Also the highest β-carotene concentration was from Umuspo 3 flesh sample planted 30 and 40 cm (409.45 and 441.15 mg/100g) and the peel for samples planted 30 and 40 cm (490.47 and 640.69 mg/100g, respectively) at the 12th week of harvest. Flavonoids were present in significant amounts (310.62mg/100g) in Umuspo 3 planted at 30 cm and harvested after 12th week while in total polyphenol, significant quantities of ≈42.12mg/100g was present in Ex-Igbariam spaced at 30 cm and 40 cm and harvested after 16th week. Provitamin A carotenoid was calculated and Umuspo 3 pro-vitamin A carotenoid was significantly higher (p< 0.05) with highest concentration (742.26 RE/100g) present in samples from 40 cm planting distance. The results showed that planting space and harvesting period had significant impact on the carotenoid and phytochemical concentrations of OFSP varieties. Planting distances of 30 and 40 cm are recommended for high carotenoid content in the two sweet potato varieties.
The aim of this study was to determine the impact of sex on the content of β-carotene, lutein, astaxanthin, canthaxanthin, zeaxanthin, and total carotenoids in the skin, muscles, and livers of whitefish (Coregonus lavaretus lavaretus (L.)). Fish samples for assays were collected from Lake Łebsko. There is a whitefish conservation program in Lake Łebsko. The results show that males are more carotenoid rich in comparison to females. The total carotenoids ranged from 0.39 μg g⁻¹ wet weight (ww) (skin of females) to 1.55 μg g⁻¹ ww (livers of males). The highest carotenoid levels were in whitefish fivers (1.36 - 1.55 μg g⁻¹ ww), and the lowest were in its skin (0.39 - 0.41 μg g⁻¹ ww). Astaxanthin was the dominant carotenoid in whitefish muscles and fivers. The highest levels of β-carotene were recorded in the skin of both whitefish males and females.
Popularisation of historical rose cultivars is important for overall increase of biodiversity within currently available assortment, however their vegetative propagation is rather difficult. Possible scenario behind it is that commonly used one-bud leafy cuttings have limited storage capacity due to the small size of the stem. We hypothesised that the treatment of cuttings this rose with rooting preparations would positively affect on the condition of their leaves. We tested the effect of preparations based on seaweed extracts (Bio Rhizotonic, Root Juice, Bio Roots) as well as commonly used root stimulants (Ukorzeniacz Aaqua and Ukorzeniacz Baqua containing 0,4% IBA or 0,2% NAA respectively) on the viability potential of the Rosa ‘Duchesse d’Angoulême’ cuttings. We have monitored the senescence process in leaves by the total soluble protein, chlorophyll and carotenoid content measurements. In order to test the potential effect of the mother plant and timing of shoots acquirement cuttings for the experiment were taken from mother plants at four different maturity stages (flower buds closed; all flowers open; just after petal fall and 7–14 days after petal fall). As expected the amount of the measured components in mother plants varied in individual phases of shoot maturity. This feature influenced the positive effect of rooting stimulants on cuttings viability. The most striking effects were in rooted cuttings derived from shoots collected just after flower petal fall. Observed increase in soluble protein and chlorophyll content did not correlate however with the percentage of obtained rooted cuttings. Based on that we can speculate that the biological basis of observed positive effect of natural rooting preparations on the ‘Duchesse d’Angoulême’ rose is complex and does not rely on the delay or inhibition of senescence process.
The aim of the research conducted in 2010-2012 on one-year, two-year and three-year shoots of three verities of Salix viminalis: ‘Bjor’, ‘Jorr’ and ‘Tora’, grown from 14, 15 and 16 year old stumps, was to compare the shoots of different age in terms of selected physiological characteristics, that is the concentration of chloroplast pigments, water balance of leaves and water content and dry matter of shoots. The content of chloroplast pigments (chlorophyll a, chlorophyll b and carotenoids) and the water balance were determined on the basis of RWC-relative water content and WSD-water saturation deficit indicators. Moreover, the water and dry matter content of one-year, two-year and three-year old willow shoots were determined. It was found that the age of the shoots differentiated the physiological parameters of willow varieties. The highest concentration of chlorophylls was reported in the leaves of three-year old shoots of the Bjor variety, in turn the highest concentration of carotenoids was found in the leaves of one-year old shoots of Jorr. Regardless of the age of the shoots, the highest rate of hydration of leaves and stems was a characteristic trait of the Tora variety, and the lowest was found in Jorr. High levels of the measured physiological parameters, such as chlorophyll a and b, carotenoids and relative water content in leaves obtained in the 5th rotation, that is 14th, 15th and 16th year of running the plantation, indicate a high physiological activity and hence high productivity of the studied willow varieties.
Carotenoids (neoxanthin, violaxanthin, lutein and β-carotene) present in plant cell play a role in photoprotection. Industrial pollution causes oxidative stress in plants, while carotenoids react with free radicals and dissipate the excess of excitation energy. In this way carotenoids prevent the negative influence of free radicals on metabolism and can even restore some of the damages. This is confirmed by results of our analysis of the level of xanthophylls in 16 and 17-year-old trees of Scots pine (Pinus sylvestris L.). Developed from seed collected in north-eastern, the trees grow in a relatively unpolluted control site, and in a polluted site located 2 km for away from the Phosphorus Fertilizer Works. In the polluted site the environment is contaminated with SO2, NOx and F, Al, F, Pb, Cu. The needles analysed in this study were visually undamaged. Material was collected in experimental plot from 6 trees in October'98 and April'99, between 12.00 and 13.00 hours, at full sunlight. The pigments were analysed quantitatively and qualitatively by thin-layer chromatography. The paper presents results of content and distribution of neoxanthin, violaxanthin, lutein and β-carotene of Scots pine needles from a healthy control tree and a stressed tree. Marked differences in pigment levels depended on the stage of needle development and level of pollution.
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