Wyniki wyszukiwania

1

Preliminary evidences to action of calmodulin on CuZn-iso-superoxide dismutases from cucumber cotyledons

100%

Chong K., Ting L., Yang C.

Acta Physiologiae Plantarum

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1993

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tom 15

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nr 1

2

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Effects of calmidazolium, carbachol and derivatives of cyclic GMP on the longitudinal internal resistivity in rabbit atrial trabeculae

75%

Tuganowski W.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

The effects of calmidazolium, carbachol and membrane permeable derivatives of cGMP (dipalmitoyl cGMP and 8-Bromo cGMP) on the longitudinal internal resistivity (Ri) were studied in the rabbit atiial trabeculae by means of electrophysiological recording techniques and histological planimetry. Calmidazolium as well as carbachol decreased Ri whereas cGMP-derivatives enhanced this resistivity. The effect of calmidazolium suggested that calmodulin reduced the cell coupling under control conditions. Carbachol decreased the Ca-inward current, and probably it prevented the calmodulin activation. The action of the nucleotides showed that cGMP did not mediate the cholinergic effect on the cell coupling. The possible interaction between calmodulin and cGMP was discussed.

3

Phytochrome and UV signal transduction pathways

75%

Bowler C., Frohnmeyer H., Schafer E., Neuhaus G., Chua N. H.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 4

The phytochromes are the best studied plant photoreceptors, controlling a wide variety of responses at both whole plant and single cell levels. Three signal transduction pathways, dependent on cGMP and/or calcium, have been found to be utilized by phytochrome to control the expression of genes required for chloroplast development (e.g., CAB and FNR) and anthocyanin biosynthesis (e.g., CHS). In particular, cGMP is a second messenger positively regulating CHS gene expression whilst calcium and calmodulin act as negative regulators. In addition to phytochrome regulation of CHS we have begun to examine the signal transduction pathways utilized by UV photoreceptors. In contrast to phytochrome-mediated responses, results indicate a role for calcium and calmodulin as positive regulators of CHS gene expression in UV light.

4

Rapid systemic up-regulation of genes after heat-wounding and electrical stimulation

75%

Davies E., Vian A., Vian C., Stankovic B.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 4

When one leaf of a tomato plant is electrically-stimulated or heat-wounded, proteinase inhibitor genes are rapidly up-regulated in distant leaves. The identity of the systemic wound signal(s) is not yet known, but major candidates include hormones transmitted via the phloem or the xylem, the electrically-stimulated self-propagating electrical signal in the phloem (the action potential, AP), or the heat-wound-induced surge in hydraulic pressure in the xylem evoking a local change in membrane potential in adjacent living cells (the variation potential, VP). In order to discriminate between these signals we have adopted two approaches. The first approach involves applying stimuli that evoke known signals and determining whether these signals have similar effects on the “model” transcripts for proteinase inhibitors (pin) and calmodulin (cal). Here we show that a heat wound almost invariably evokes a VP, while an electrical stimulation occasionally evokes an AP, and both of these signals induce accumulation of transcripts encoding proteinase inhibitors. The second approach involves identifying the array of genes turned on by heat-wounding. To this end, we have constructed a subtractive library for heat-wounded tissue, isolated over 800 putatively up-regulated clones, and shown that all but two of the fifty that we have analyzed by Northern hybridization are, indeed, up-regulated. Here we show the early kinetics of up-regulation of three of these transcripts in the terminal (4th) leaf in response to heat-wounding the 3rd leaf, about 5 cm away. Even though these transcripts show somewhat different time courses of induction, with one peaking at 30 min, another at 15 min, and another at 5 min after flaming of a distant leaf, they all exhibit a similar pattern, i.e., a transient period of transcript accumulation preceding a period of transcript decrease, followed by a second period of transcript accumulation.

5

Brain spectrin and friends

63%

Riederer B. M., Wintergerst E. S., Valcourt E., Routtenberg A.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 1

Brain spectrin (fodrin, calspectin), is an actin binding protein, and composed of two α- and two β-subunits which combine by head-to-head interaction to a heterotetramere. Together with other proteins it forms a proteinaceous meshwork underlying the cytoplasmic surface of the plasma membrane. In the mammalian brain, three forms of spectrins were identified, an axonal, a somato-dendritic and a astroglial one. A subcellular localization at the electron microscopic-level suggests that these forms are not only attached to membranes but expand into the cytoplasm. It was shown that they differ in their subcellular distribution, and their temporal appearance during postnatal brain development. The subplasmalemmal cytoskeleton is composed of brain spectrin and a variety of proteins such as actin and calmodulin, and we discuss here two additional proteins, FI and parvalbumin, which may bind to brain spectrin and may have to be included in the circle of proteins interacting with brain spectrin.

6

Biphasic enhacement of nitric oxide synthase activity and cGMP level following brain ischemia in gerbils

63%

Strosznajder J., Chalimoniuk M.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 1

7

Changes of Ca 2-calmodulin-dependent protein kinase-II after transient ischemia in gerbil hippocampus

63%

Zalewska T., Zablocka B., Domanska-Janik K.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 1

8

Calmodulin and calmodulin-like protein are involved in pollen-pistil interaction: Immunocytochemical studies on Petunia hybrida Hort.

63%

Lenartowska M., Rodriguez-Garcia M. I., Bednarska E.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

A commercial anti-calmodulin monoclonal antibody (CaM MAb) was used to determine the presence and localization of calmodulin (CaM) and calmodulin-like protein in unpollinated and pollinated styles of Petunia hybrida. In the unpollinated style , CaM was localized in the cytoplasm of transmitting tract cells. The response to pollination and/or pollen tube growth was the presence of bound CaM-like protein in the extracellular matrix (ECM) of transmitting tissue. Gold particles were localized in the ECM surrounding the tips of the growing pollen tubes. In the pollen tubes, CaM epitopes were present mainly in and around some vesicles of the apical cytoplasm. The results suggest that CaM and CaM-like protein are involved in pollen tube growth in vivo.

9

Ion efflux and changes of external pH in early responses of excised cucumber cotyledons to kinetin and calmodulin inhibitors

63%

Szweykowska A., Garnczarska M.

Acta Physiologiae Plantarum

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1993

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tom 15

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nr 2

10

Ciliary and flagellar activity control in eukaryotic cells by second messengers: calcium ions and cyclic nucleotides

63%

Fabczak H., Walerczyk M., Sikora J., Fabczak S.

Acta Protozoologica

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1999

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tom 38

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nr 2

11

Energy metabolism in plants under water deficits

63%

Zagdanska B.

Acta Biochimica Polonica

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1995

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tom 42

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nr 3

Regardless of the mechanisms of stress resistance, the maintenance of functional integrity in plants imposes an energetic cost. This mini-review summarized the present knowledge on energy metabolism in plants under drought conditions with the focus on studies performed on wheat in author's laboratory.

12

Changes of U937 cell viability induced by stress factors - possible role of calmodulin

51%

Wojcik-Piotrowicz K., Kaszuba-Zwoinska J., Rokita E., Nowak B., Thor P.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 4

13

Regulation of Ca2plus release from internal stores in cardiac and skeletal muscles

51%

Wrzosek A.

Acta Biochimica Polonica

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2000

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tom 47

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nr 3

It is widely accepted that Ca2+ is released from the sarcoplasmic reticulum by a specialized type of calcium channel, i.e., ryanodine receptor, by the process of Ca2+-induced Ca2+ release. This process is triggered mainly by dihydropyridine receptors, i.e., L-type (long lasting) calcium channels, directly or indirectly interacting with ryanodine receptor. In addition, multiple endogenous and exogenous compounds were found to modulate the activity of both types of calcium channels, ryanodine and dihydropyridine receptors. These compounds, by changing the Ca2+ transport activity of these channels, are able to influence intracellular Ca2+ homeostasis. As a result not only the overall Ca2+ concentration becomes affected but also spatial distribution of this ion in the cell. In cardiac and skeletal muscles the release of Ca2+ from internal stores is triggered by the same transport proteins, although by their specific isoforms. Concomitantly, heart and skeletal muscle specific regulatory mechanisms are different.

14

Purification of the Ca2 -ATPase from rat cortical, cerebellar and hippocampal synaptosomal membranes by two - step chromatography

51%

Zylinska L., Lachowicz L., Gromadzinska E.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 1

Ca2+-ATPase, the enzyme responsible for maintenance of low resting Ca2+ level in the nerve cell, has been purified from rat cortical, cerebellar and hippocampal synaptosomal membranes by affinity chromatography on Calmodulin-Agarose which followed Reactive Red 120-Agarose column. The enzymes from these three regions ran as broad, monomeric bands on SDS-PAGE with a molecular weight at range 130-138 kDa, and were identified as a Ca2+- ATPase using monoclonal antibody 5F10. Analysis of the kinetic parameters revealed that hippocampal Ca2+-ATPase exhibited 2 times higher affinity for ATP, than cortical and cerebellar enzymes. The affinity for Ca2+ increased in order: cerebellum, cortex, and hippocampus. The differences in kinetic characteristics of purified enzymes, suggest that in adult rat brain the Ca2+-ATPase could be represented at a protein level by the region-dependent combination of several isoforms.

15

Spinal effects of the calmodulin inhibitor calmidazolium on dorsal horn neurons in the rat

51%

Menendez L., Baamonde A., Hidalgo A.

Acta Neurobiologiae Experimentalis

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1999

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tom 59

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nr 1

16

Immunocytochemical localization of calmodulin in fertile and sterile anther of Allium cepa L.

45%

Golaszewska B., Bednarska E., Narbutt O.

Acta Biologica Cracoviensia. Series Botanica

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2000

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tom 42

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nr 1

Calmodulin distribution was studied in the maturing male fertile and male sterile anther of Allium cepa L., using commercial monoclonal anti-CaM antibodies. The main sites of its occurrence were the tapetum and the generative cell lines. During microsporogenesis, calmodulin localization in the anthers of both onion lines was similar. Anti-CaM was localized in dividing microsporocytes, and low levels of it were found in the tapetum cytoplasm. Significant differences in calmodulin level and distribution between male fertile and male sterile anthers were observed after completion of meiosis. In the tapetum of the male fertile anther, throughout its existence, calmodulin level was higher than in the tapetum of the male sterile anther. The prematurely degenerating male sterile tapetum, starting from the free-microspore stage, did not bind anti-CaM. Differences in calmodulin localization between the functional and nonfunctional tapetum were found already in the tetrad stage, that is, before the start of degeneration of microspores. In functional tapetum cells, anti-CaM was concentrated in the cytoplasm on the loculus side; in the nonfunctional tapetum of the male sterile anther, calmodulin was evenly distributed over the cells. In the microspores of the male sterile line, calmodulin was dispersed in the cytoplasm. During pollen maturation, calmodulin was observed to undergo polarization - anti-CaM was localized mainly in the region of one of the pollen poles, near the plasma membrane. In the male sterile anther, progressive degeneration of microspores was associated with decreasing calmodulin level in the cytoplasm; microspores in the abortion stage did not bind anti-CaM.

Ograniczanie wyników

Preliminary evidences to action of calmodulin on CuZn-iso-superoxide dismutases from cucumber cotyledons

Effects of calmidazolium, carbachol and derivatives of cyclic GMP on the longitudinal internal resistivity in rabbit atrial trabeculae

Phytochrome and UV signal transduction pathways

Rapid systemic up-regulation of genes after heat-wounding and electrical stimulation

Brain spectrin and friends

Biphasic enhacement of nitric oxide synthase activity and cGMP level following brain ischemia in gerbils

Changes of Ca 2-calmodulin-dependent protein kinase-II after transient ischemia in gerbil hippocampus

Calmodulin and calmodulin-like protein are involved in pollen-pistil interaction: Immunocytochemical studies on Petunia hybrida Hort.

Ion efflux and changes of external pH in early responses of excised cucumber cotyledons to kinetin and calmodulin inhibitors

Ciliary and flagellar activity control in eukaryotic cells by second messengers: calcium ions and cyclic nucleotides

Energy metabolism in plants under water deficits

Changes of U937 cell viability induced by stress factors - possible role of calmodulin

Regulation of Ca2plus release from internal stores in cardiac and skeletal muscles

Purification of the Ca2 -ATPase from rat cortical, cerebellar and hippocampal synaptosomal membranes by two - step chromatography

Spinal effects of the calmodulin inhibitor calmidazolium on dorsal horn neurons in the rat

Immunocytochemical localization of calmodulin in fertile and sterile anther of Allium cepa L.