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1
Content available remote

Influence of the "open field" exposure on calbindin-D28K, calretinin, and parvalbumin containing cells in the rat midbrain - developmental study

100%
Klejbor I., Ludkiewicz B., Domaradzka-Pytel B., Spodnik J. H., Dziewiatkowski J., Morys J.
Journal of Physiology and Pharmacology
|
2006
|
tom 57
|
nr 1
1. Distribution of c-Fos positive nuclei in: ventral tegmental area, substantia nigra, periaqueductal gray. 2. Appearance of calbindin-D28k, calretinin and parvalbumin in midbrain neurons that are engaged in the stress response. 3. Changes of c-Fos and calcium-binding proteins expression during maturation. The material consisted of Wistar rats of age between 0 and 90 days. The OF exposure was applied throughout 10 min and 90 min before the death of the animals. The brain sections were double stained using the antibodies against c-Fos, CB, CR or PV. Our results showed that in all studied nuclei age-related increase of c-Fos expression (without changing of its distribution properties) was found. PV didn't show any co-localization with c-Fos in neurons of studied regions at any ages, however some PV-immunoreactive (PV-ir) basket-like structures around c-Fos-immunoreactive (c-Fos-ir) neurons were observed. In the youngest group of rats c-Fos-ir cells and cells immunoreactive for CB and CR constituted separate neuronal populations. During maturation increases in the level of their co-localization with c-Fos was observed. We may conclude that in adult rat midbrain structures CB-immunoreactive (CB-ir) and CR-immunoreactive (CR-ir) cells (probably projection neurons) are mainly activated in the stress response following OF exposure. In the contrary PV-ir cells has only an indirect (modulatory) influence upon the c-Fos-ir cells.
2

Calcium-binding proteins immunoreactivity in the preoptic area of the guinea pig (Cavia porcellus) during prenatal development

86%
Hermanowicz B., Bogus-Nowakowska K., Zakowski W., Robak A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2014
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tom 56
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nr 1
3

p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein [GCAP]

86%
Dejda A., Matczak I., Gorczyca W. A.
Acta Biochimica Polonica
|
2002
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tom 49
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nr 4
The Ca2+ -dependent activation of retina-specific guanylyl cyclase (retGC) is medi­ated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 uM) concentrations of Ca2+ .AtlowCa2+ , immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat reti­nal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+ -dependent electrophoretic mobility shift.
4

Cation binding properties of calretinin, an EF-hand calcium-binding protein

86%
Groves P., Palczewska M.
Acta Biochimica Polonica
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2001
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tom 48
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nr 1
Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in some neurodegenerative diseases, although it is as yet unconfirmed how CR plays an active role in this protection. Higher levels of some metal cations (e.g. copper and zinc) are associated with these diseases. At the same time, metals such as terbium (NMR and fluorescence) cadmium (NMR) and manganese (EPR) serve as useful calcium analogues in the study of EF-hand proteins. We survey the binding of the above-mentioned metal cations that might affect the structure and function of CR. Competitive 45Ca2+ -overlay, competitive terbium fluorescence and intrinsic tryptophan fluorescence are used to detect the binding of metal cations to CR. Terbium and copper (half-maximal effect of 15 uM) bind to CR. Terbium has a similar or greater affinity for the calcium-binding sites of CR than calcium. Copper quenches the fluorescence of terbium-bound CR, and CR tryptophan residues and com­petes weakly for 45Ca2+ -binding sites. Cadmium, magnesium, manganese and zinc bind less strongly (half-maximal effects above 0.1 mM). Therefore, only terbium appears to be a suitable analytical calcium analogue in further studies of CR. The principal conclusion of this work is that copper, in addition to calcium, might be a factor in the function of CR and a link between CR and neurodegenerative diseases.
5
Content available remote

Open field stress and neurons containing calcium-binding proteins in the piriform cortex of the rat

86%
Klejbor I., Ludkiewicz B., Domaradzka-Pytel B., Wojcik S., Morys J.
Journal of Physiology and Pharmacology
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2005
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tom 56
|
nr 2
In the present study we wanted to check whether the expression of the c-Fos protein (the marker of cellular activity) appears in cells containing calcium-binding proteins (CaBPs) in animals exposed to the open field test. Eight adult Wistar rats were examined. In the first step the open field test was applied throughout 10 minutes. After perfusional fixation brains were frozen and cut on the cryostat in the coronal plane and stained with the standard immunohistochemical method. Sections were double stained for c-Fos and CaBPs: parvalbumin (PV), calbindin (CB), calretinin (CR). c-Fos positive cells were localized predominantly in layers II and III of the piriform cortex (PC). The double labeling study showed that neurons containing CaBPs are rarely c-Fos-immunoreactive. Often PV-positive and CB-positive fibers surround c-Fos-positive neurons in layers II and III in a form of a basket. It seems that cells containing CaBPs are not directly involved in the response to aversive stimuli but cells containing those calcium-binding proteins might influence directly c-Fos positive neurons of PC.
6

Co-localisation of NOS with calcium-binding proteins during the postnatal development of the rat claustrum

72%
Kowianski P., Morys J. M., Wojcik S., Dziewiatkowski J., Morys J.
Folia Morphologica
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2003
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tom 62
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nr 3
211-214
An immunocytochemical double-staining method was applied in order to study the co-localisation of nitric oxide synthase (NOS) with three calcium-binding proteins, calbindin D28k (CB), calretinin (CR) and parvalbumin (PV) in the claustrum of the rat during the first 4 months of life (postnatal days: P0–P120). The co-localisation of NOS/PV and NOS/CB is reported. These neurons fall into the category of non-pyramidal cells. Double-labelled NOS/CB neurons are observed in the claustrum starting from P4, whereas double-labelled NOS/PV neurons are observed from P14 onwards. The percentages of double-labelled neurons increase in relation to the age. Double-labelled NOS/CB and NOS/PV neurons, although they do not constitute a numerous population, play an important role in the process of maturation of the claustrum. This is confirmed by the occurrence of these types of neurons at definite stages of maturation and by the increase in their number.
7

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

72%
Rycerz K., Krawczyk A., Jaworska-Adamu J., Krawczyk-Marc I.
Folia Histochemica et Cytobiologica
|
2014
|
tom 52
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nr 4
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases

72%
Bucholc M., Goch G., Ciesielski A., Anielska-Mazur A., Dobrowolska G.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 2
9

Analysis of calretinin immunoreactivity in the rat piriform cortex after open field stress during postnatal maturation

72%
Badowska-Szalewska E., Klejbor I., Ludkiewicz B., Morys J.
Folia Morphologica
|
2005
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tom 64
|
nr 1
33-40
In our study we used c-Fos protein to identify whether cells containing calretinin (CR) in the rat piriform cortex are engaged in the response to stress stimulation and to find out how this expression changes during maturation (PC). The material consisted of Wistar strain rats of between 0 and 120 days of age divided into 9 groups. Each group consisted of 5 experimental and 3 control rats. Animals from the experimental groups were exposed to the open field test throughout 10 minutes. The control animals were kept in a home cage. In all age-related control rats weak c-Fos immunoreactivity was observed. Our results showed that cells containing c-Fos following an acute open field test were observed predominantly in layers II and III of the PC just after birth. Their number then increased and stabilised on P30. We had already observed immature CR-ir cells at birth. In the 4th week of life these neurons achieved maturity. Their number increased to P90 and decreased in older animals. CR-ir neurons were localised mainly in layer II and to a lesser degree in layers III and I of the PC. Double immunostaining c-Fos/CR revealed that the level of co-localisation was low. Only small differences were observed between the anterior and posterior parts of the PC. In the anterior part a higher number of CR-ir neurons was found. The difference in the level of co-localisation between the anterior and posterior parts was age-related and differentiated. Our results may suggest that during maturation CR-ir neurons of the piriform cortex are not the main population engaged in response to the open field test.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Molecular mechanisms of lead toxicity

72%
Szymanski M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2014
|
tom 95
|
nr 2
11

Reactivity of astrocytes in the periaqueductal gray matter of rats treated with monosodium glutamate

72%
Krawczyk A., Jaworska-Adamu J.
Folia Histochemica et Cytobiologica
|
2020
|
tom 58
|
nr 2
12

Ca2plus differently affects hydrophobic properties of guanylyl cyclase-activating proteins [GCAPs] and recoverin

72%
Gorczyca W. A., Kobialka M., Kuropatwa M., Kurowska E.
Acta Biochimica Polonica
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2003
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tom 50
|
nr 2
Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+ -binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+ . Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+ -dependent binding of recoverin to Phenyl-Se- pharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+ ]free (10 uM) than at low [Ca2+ ]freee(10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+ ]free in recoverin and at low [Ca2+ ]freee in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ ap­pear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.
13

Photoreceptor guanylate cyclase variants: cGMP production under control

72%
Sokal I., Alekseev A., Palczewski K.
Acta Biochimica Polonica
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2003
|
tom 50
|
nr 4
Changes in the Ca2+ concentration are thought to affect many processes, including signal transduction in a vast number of biological systems. However, only in few cases the molecular mechanisms by which Ca2+ mediates its action are as well understood as in phototransduction. In dark-adapted photoreceptor cells, the equilibrium level of cGMP is maintained by two opposing activities, such as phosphodiesterase (PDE) and guanylate cyclase (GC). Upon absorption of photons, rhodopsin-G-protein- mediated activation of PDE leads to a transient decrease in [cGMP] and subsequently to lowering of [Ca2+]. In turn, lower [Ca2+] increases net production of cGMP by stimulation of GC until dark conditions are re-established. This activation of GC is mediated by Ca2+-free forms of Ca2+-binding proteins termed GC-activating proteins (GCAPs). The last decade brought the molecular identification of GCs and GCAPs in the visual system. Recent efforts have been directed toward understanding the properties of GC at the physiological and structural levels. Here, we summarize the recent progress and present a list of topics of ongoing research.
14

Present and future use of isotopes as shown in studies on calcium-binding proteins

72%
Kuznicki J.
Bulletin of the Polish Academy of Sciences. Biological Sciences
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1998
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tom 46
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nr 3-4
15

Cholinergic endings on various neurones containing calcium binding proteins and glutamic acid decarboxylase in the hippocampus of the rat

72%
Ludkiewicz B., Wojcik S., Spodnik E., Domaradzka-Pytel B., Morys J.
Folia Morphologica
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2000
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tom 59
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nr 4
Immunohistochemical study of the cholinergic innervation of the hippocampal cells containing glutamic acid decarboxylase (GAD) and calcium binding proteins: parvalbumin (PV), calbindin D28k (CB) and calretinin (CR) was conducted on 5 adult rat brains. Analysis of sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and respectively either GAD or PV, CB, CR, using confocal laser-scanning microscope shows that the intensive cholinergic innervations receive GAD, PV and CB-positive hippocampal cells. Cholinergic afferentations of the CR-positive neurones are considerably fewer.
16

Calcium-binding proteins immunoreactivity during ontogenesis of the limbic structures in the guinea pig

58%
Robak A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2014
|
tom 56
|
nr 1
17

Correlation between dopaminergic phenotype and expression of calretinin in the midbrain nuclei of the opossum (Monodelphis domestica): An immunohistological study

58%
Klejbor I., Ludkiewicz B., Wojcik S., Turlejski K.
Acta Neurobiologiae Experimentalis
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2013
|
tom 73
|
nr 4
We investigated distribution and morphology of neurons of the midbrain nuclei: the ventral tegmental area (VTA), substantia nigra (SN) and periaqueductal gray (PAG) of the adult grey short-tailed opossums that were double immunolabeled for the presence of calretinin (CR) and/or tyrosine hydroxylase (TH). The majority of TH-immunopositive neurons and fibers were located in the VTA, SN, and only scarce population of small neurons expressing TH was present in the PAG. In the SN 80% of TH-expressing neurons had large cell bodies, and only a small fraction had small perikarya. In the PAG populations of large and medium sized neurons were equal and 20% of neurons had small perikarya. Much scarcer population of TH-immunoreactive neurons in the PAG consisted of large or small neurons in its dorsal part (PAGd) and almost exclusively small neurons in the ventral part (PAGv). Distribution of neurons expressing TH and their types in the opossum are similar to those in rodents. The majority of CR-immunolabeled neurons were found in the VTA. In its subdivision, the parabrachal pigmented nucleus (PBP) cells expressing CR were approximately 28% more numerous than cells expressing TH. In spite of that, only 42% of TH-expressing neurons coexpressed CR. The high degree of colocalization TH and CR was observed in the SN. We propose that a higher percentage of TH/CR colocalization, which is observed in the opossums SN, may give them the ability to adapt to changes in their motor functions.
18

Redox potential influences oligomerization state of annexin 1 [AnnAt1] from Arabidopsis thaliana

58%
Gorecka K. M., Konopka-Postupolska D., Henning J., Pikula S.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
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