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The identity of the original material of Juncus kochii F. W. Schultz (Juncaceae) is discussed. The taxon at present is best regarded as Juncus bulbosus L. subsp. kochii (F. W. Schultz) Reichg. The relevant literature (protologue and references therein) was searched and details of all original elements were compiled. Nomenclatural and taxonomic remarks are given. Juncus welwitschii Hochst. ex Steud. is here excluded as a synonym of Juncus bulbosus subsp. kochii.
The identity of the potential original material of Juncus kochii is discussed. The taxon at present is best regarded as Juncus bulbosus L. subsp. kochii (F.W. Schultz) Reichg. The relevant literature (protologue and references therein) was searched and details of all residual original elements were compiled. Taxonomic remarks are given. The herbarium material from the environs of Bitche (Moselle, Lorraine) and Gérardmer (Vosges, Lorraine) – both in France, has been rejected as useless for typification of J. kochii (because of taxonomic reasons), though it was listed both in the protologue and also as indirect references therein Schultz’s earlier publications.
The first localities of Juncus bulbosus (Juncaceae) for South America (Chile) and for the whole south-western part of the world (S latitude and E longitude) are described, including precise geographical location and climatic conditions. General remarks on the invasiveness of the species and on the possibility of finding it in other parts of the world are given. The distribution of the species world-wide requires further studies, since the bulbous rush has become an invasive plant in areas where it did not occur before.
Autumn daffodil (Sternbergia lutea) is a rare and endangered bulbous plant with yellow flowers grown naturally in Iran and unfortunately for different reasons this plant is at the risk of eradication. Natural multiplication rate of S. lutea is low, thus propagation by chipping method is one inexpensive and simple way to overcome this problem. This research was therefore performed to investigate the propagation method in two experiments. In the first experiment, the effects of some plant growth regulators (PGRs) on induction and regeneration of bulblets from bulb chips (propagules) were evaluated. The propagules were prepared by chipping method and treated with indole butyric acid (IBA), gibberellic acid (GA3), benzyl adenine (BA), kinetin (Kin) and cycocel (CCC) at 100 and 200 mg L–1 concentrations for 2 h and then incubated for two months at 20 ±1°C. At the end of incubation, the results showed the highest percentage of concurrent bulblet + root regeneration (CBRR), number and fresh weight of bulblets related to 100 mg L–1 GA3. The highest percentage of only bulblet regeneration (OBR), only root regeneration (ORR) and non-regeneration were obtained at 200 mg L–1 IBA, 100 mg L–1 Kin and control (distillated water: DW) treatments, respectively. In the second experiment, the bulblets that had rooted from each treatment of the first experiment were planted and the produced bulbs were compared. The results indicated that the highest percentage of bulblet sprouting, bulb number and diameter were found at 100 mg L–1 GA3. Therefore, the application of GA3 with mentioned concentration is appropriate for vegetative propagation of this plant by bulb chipping.
Tuberose (Polianthes tuberosa L.) is an ornamental bulbous plant and a famous cut flower in tropical and subtropical regions. Post-harvest senescence of the cut flowers is the main factor limiting the marketability of most of these species including tuberose. From the perspective of metabolic changes, senescence happens as the result of oxidative processes induced by active oxygen species production. Gamma-aminobutyric acid (GABA) and salicylic acid (SA) are compounds with some functions in the post-harvest physiology of some plants. The present study focused on the effect of GABA and SA on vase life and some post-harvest traits of cut tuberose flowers. The plants were sprayed with GABA (5, 10, or 15 mg L–1) and SA (50, 100, or 150 mg L–1) at three stages during growth and before harvest in a greenhouse (30, 45, and 60 days after the planting of the bulbs) and were observed after harvest until senescence. Results showed that GABA and SA positively affected the vase life, water uptake, fresh weight, ion leakage, total dissolved solids, chlorophyll, protein, and catalase, peroxidase, and ascorbate peroxidase enzymatic activity. They postponed senescence. The highest and lowest vase lives were observed in plants treated with 10 mg L–1 GABA (11 days) and control (distilled water) (7 days), respectively. It was found that the treatment of tuberose with GABA and SA during growth can improve its post-harvest quality. However, it is recommended to conduct further studies on them.
Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
Shoot and bulb induction of hyacinths under the influence of varying light treatments (blue, green, yellow, red, far-red and UV irradiation) was studied in long-term in vitro cultures. Leaf explants were cultivated on solid MS medium containing sucrose or glucose (90, 180 or 360 mM). The light as well as the types and concentrations of carbohydrates affected regeneration. Blue light stimulated growth and development of adventitious shoots and buds regardless of carbohydrate type, while the highest number of bulbs was obtained under red or white light and darkness as well as low and moderate concentrations of sucrose in medium. On average, 360 mM glucose yielded the highest proliferation rate of adventitious bulbs, followed by 180 mM sucrose. Blue light and UV irradiation promoted development of chlorophylls. Blue light also increased the total amount of anthocyanins. Far-red light reduced chlorophyll content.
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