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1

Serodiagnosis of Fasciola gigantica infection in buffaloes with native cathepsin-L proteases and recombinant cathepsin L1-D

100%
Aftab A., Lall R., Bisen S., Anandanarayanan A., Rialch A., Chamuah J. K., Yadav S., Silamparasan M., Raina O. K.
Acta Parasitologica
|
2020
|
tom 65
|
nr 2
2

Seroprevalence of leptospiral infection in buffalo [Bubalus bubalis]

100%
Hajikolaei M. R. H., Ghorbanpour M., Abdollapour G.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 3
3

Prevalence of subclinical coccidiosis in river buffalo calves of Southwest of Iran

84%
Bahrami S., Alborzi A. R.
Acta Parasitologica
|
2013
|
tom 58
|
nr 4
Despite the importance of buffalo farming in Iran, little is known in this country about the abundance and distribution of Eimeria spp. in the animal species. The present study was designed to investigate the prevalence and species characterization of Eimeria oocysts in river buffalo calves of Khuzestan province, southwest of Iran. Of the total 108 fecal samples examined for Eimeria, 108 (100%) were found infected with 11 species of the parasite. Among the identified species of Eimeria, E. bovis was found to be the predominant etiological agent (76.85%), followed in order by E. canadensis (62.96%), E. zuernii (47.2%), E. ellipsoidalis (26.85%), E. subspherica (25.92%), E. brasiliensis (19.4%), E. auburnensis (18.51%), E. alabamensis (14.81%), E. pellita (11.1%), E. illinoisensis (5.5%) and E. bukidnonensis (2.7%). In most calves multiple infections with three species were present. While, 20.7% of calves showed heavy infection, 50.4 and 24.8% of calves showed weak and moderate infection, respectively. There was no significant difference in the OPG values between the calves of different localities. There was also no significant difference between the prevalence rate of infection in males and females. A total of 16.6% of all faecal samples were found to be diarrheic. A highly significant relationship could be identified between the occurrence of diarrhea and the level of E. bovis and E. zuernii oocysts excretion. Considering the pervasive occurrence and negative effects of the infection on the health condition and the growth performance of buffalo calves, infections should receive increased attention by both farmers and veterinarians.
4

Molecular assessment of Anaplasma marginale in bovine and Rhipicephalus (Boophilus) microplus tick of endemic tribal belt of coastal South Gujarat, India

84%
Kumar N., Solanki J. B., Varghese A., Jadav M. M., Das B., Patel M. D., Patel D. C.
Acta Parasitologica
|
2019
|
tom 64
|
nr 4
5

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

84%
Li X., Li M., Huang S., Qiao S., Qin Z., Kang C., Shi D.
Cellular and Molecular Biology Letters
|
2014
|
tom 19
|
nr 4
CD14 plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, resulting in inflammation. Upstream inhibition of the inflammation pathway mediated by bacterial LPS, toll-like receptor 4 (TLR4) and cluster of differentiation antigen 14 (CD14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. To explore the effect of CD14 downregulation on the expression of TLR4 signaling pathway-related genes after LPS stimulation in buffalo (Bubalus bubalis) monocyte/macrophages, effective CD14 shRNA sequences were screened using qRT-PCR and FACS analysis with buffalo CD14 shRNA lentiviral recombinant plasmids (pSicoRGFP-shRNA) and buffalo CD14 fusion expression plasmids (pDsRed-N1-buffalo CD14) co-transfected into HEK293T cells via liposomes. Of the tested shRNAs, shRNA-1041 revealed the highest knockdown efficiency (p < 0.01). When buffalo peripheral blood monocyte/macrophages were infected with shRNA-1041 lentivirus and stimulated with LPS, the expression of endogenous CD14 was significantly decreased by CD14 shRNA (p < 0.01), and the mRNA expression levels of TLR4, IL-6 and TNF-α were also significantly downregulated compared to the control groups (p < 0.01). These results demonstrated that the knockdown of endogenous CD14 had clear regulatory effects on the signal transduction of TLR4 after stimulation with LPS. These results may provide a better understanding of the molecular mechanisms of CD14 regulation in the development of several buffalo diseases.
6
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Content available

Decreased antibiotic susceptibility and enhanced probiotic production potential of goat milk fermented curd in comparison with cow and buffalo milk

84%
Lakhanpal J., Gupta J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2017
|
tom 98
|
nr 2
7

Effect of straw size and thawing time on quality of cryopreserved buffalo (Bubalus bubalis) semen

84%
Ansari M. S., Rakha B. A., Andrabi S. M. H., Akhter S.
Reproductive Biology
|
2011
|
tom 11
|
nr 1
8

Potential of Pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases

67%
Shayegh J., Atashpaz S., Salehi T., Hejazi M.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 3
Twenty-six isolates of Pasteurella multocida were recovered from cattle and buffaloes in Iran. The identification of the isolates was carried out using morphological and biochemical tests. Among eight different biochemical biovars, 17 (65.38%) of them were assigned to biovar 3 and the rest belonged to biovars 2, 4, 5, 6, 7, and 15. Capsular typing of isolates by PCR demonstrated three capsular types (A, B, and D), among which type B was the most common (46.15%) and was found in 10 (38.46%) cattle and in two (7.69%) buffalo samples. A notably high prevalence of Pasteurella filamentous haemagglutinin A (PfhA ) and transferrin binding protein encoding (tbpA) genes among three virulence genes detected by multiplex PCR were found. The high prevalence of these genes owning association of disease status among healthy animals showed high potential of the strains in induction of disease among cattle and buffalo herds. The results of this study imply that the role of environmental and host factors are more important than bacterial virulence factors (PfhA and tbpA) in disease induction.
9
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Content available

Ultrasonography as a diagnostic and prognostic approach in cattle and buffaloes with fatty infiltration of the liver

67%
Tharwat M.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 1
The aim of the present study was to determine whether ultrasonographic evaluation of the hepatic parenchyma could be used as a diagnostic and prognostic approach in cows and buffaloes with hepatic lipidosis. For this purpose, cows (n=16) and buffaloes (n=10) with fatty infiltration of the liver were examined by ultrasonography. Treated cows and buffaloes were monitored for hepatic changes ultrasonographically, biochemically and histologically. Clinical findings were non-specific and included anorexia, recumbency, muzzle necrosis, and icteric mucosal membranes. Laboratory data revealed neutrophilia, hyper γ-globulinemia, elevated activities of aspartate aminotransferase, γ-glutamyl transpeptidase, creatine kinase and lactate dehydrogenase, and high concentrations of insulin, total bilirubin, non-esterified fatty acids and β-hydroxyl butyric acid. Laboratory results 7, and 21 days after treatment showed progressive improvement in the chemistry profile. On admission, ultrasonographic examination of the hepatic parenchyma in cows and buffaloes revealed either increased or decreased hepatic echogenicity; histologic examination revealed marked fatty infiltration of the hepatocytes. One week after treatment, the hepatic parenchyma was visualized easily, liver boundaries were clearly imaged, and histologic examination of hepatic specimen showed a moderate degree of fatty infiltration. Three weeks after treatment, the hepatic parenchyma was almost similar to normal, the hepatic and portal blood vessels could be easily imaged, and the histologic picture had greatly improved where the liver resembled the normal organ. Six cows and seven buffaloes made a full recovery while the remaining ten cows and three buffaloes were slaughtered and thoroughly examined postmortem. Ultrasonography showed a good correlation with histologic and laboratory findings.
10

The use of nano-sized eggshell powder for calcium fortification of cow`s and buffalo`s milk yogurts

67%
El-Shibiny S., Abd El-Gawad M. A. M., Assem F. M., El-Sayed S. M.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2018
|
tom 17
|
nr 1
11

Serum selenium concentrations in water buffaloes (Bubalus bubalis) and black-and-white German Holstein-Friesian cows raised in the Notec River region of the Wielkopolska province

67%
Sablik P., Kobak P., Pilarczyk B., Szarkowski K., Syczewski A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Concentration of selenium in serum samples was determined using a modified Watkinson's spectrofluorometric method. Selenium concentration averaged 0.021 ±0.008 µg/mL for water buffaloes and 0.074 ±0.017 µg/mL for dairy cows. The analysis of selenium concentrations in cows showed deficiency level in 2.0%, marginal level in 64%, and optimal level in 34% of the examined samples. In water buffaloes, deficiency level was found in 96.55% and marginal level in 3.45% of the animals. Selenium levels in cattle and water buffaloes should be periodically diagnosed to provide data for veterinarians to make appropriate decisions. The implementation of suitable prophylactic programmes will enable optimum levels of this element to be maintained.
12

Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes

67%
Mishra A. K., Reddy G. G. B., Rao J. R., Tewari A. K.
Acta Parasitologica
|
1998
|
tom 43
|
nr 1
An affinity purified Babesia bigemina piroplasm antigen was successfully employed in a Dot-ELISA for detection of antibodies in buffaloes and catle. A low degree of cross-reactivity was seen against putative B. bigemina (Mexican) and B. bovis undiluted reference serum and no cross-reactivity was observed in seru dilutions of 1:10 and above. Theileria annulata did not show any reactivity with B. bigemina. The assay detected antibodies at 7, 14 or 21 days post-experimental infection in bovine calves with 37.5, 87.5 and 100% seroreactivity, respectively. The incidence of B. bigemina infections was found highest in cattle (19.91) as against 7.4% in buffaloes out of 486 sera samples, examined from Boophilus microplus infested areas from Orissa, Rajasthan and Uttar Pradesh States.
13

Sarcocystis infection of the African buffalo [Syncerus caffer] in the Kruger National Park, South Africa

67%
Quandt S., Bengis R. G., Stolte M., Odening K., Bockhardt I.
Acta Parasitologica
|
1997
|
tom 42
|
nr 2
Muscle (tongue and oesophagus) from African buffaloe, Syncerus caffer was examined for the presence of sarcocysts. Twenty-two tongue samples (66.7%) from 33 adult buffaloes (older than one year) were infected with numerous macrocysts and a few microcysts of a Sarcocystis sp. In two of these animals sarcocysts were also found in the oesophagi. Furthermore, an oesophagus from an additional older buffalo was positive. Sarcocysts were not seen in 7 buffaloes younger than one year. Light and electron microscopic investigations revealed sarcocysts with a cyst wall ultrastructure belonging to the type 21 of the classification by Dubey et al. (1989a). For the first time this type of sarcocyst is described in African buffaloes. Encapsulation of the host cell (“secondary cyst wall”), often present in other Sarcocystis species with macrocysts, was lacking. The cyst wall was morphologically similar to S. fusiformis isolated from the water buffalo (Bubalus bubalis). However, dimorphism of cystozoites, not known in S. fusiformis, was noted. Some sarcocysts had short plump, and others long bananashaped cystozoites. This phenomenon is discussed, considering also the possibility of sexual dimorphism.
14

The influence of strain, sex and age on selected biochemical parameters in blood serum of Buffalo and Wistar rats

51%
Calkosinski I., Dobrzynski M., Kobierska-Brzoza J., Majda J., Szymonowicz M., Calkosinska M., Dzierzba K., Bronowicka-Szydelko A., Soltan E., Seweryn E., Zasadowski A., Gamian A.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 2
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