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1

The study of the causal agents of massive needle fall in blue spruce

100%
Werner M., Fruzynska-Jozwiak D., Andrzejak R.
Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo
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2001
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tom 34
2
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From studies on possibility of protecting blue spruce [Picea pungens Engelm.] against fungi. Part II. Laboratory assessment of antifungal activity of selected essential oils

100%
Bartynska M., Mirski W.
Acta Scientiarum Polonorum. Hortorum Cultus
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2007
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tom 06
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nr 4
The antifungal properties of selected essential oils, i.e. lavender, mint, orange, spruce and thyme oils, were checked in vitro to fungi isolated from blue spruce (Picea pungens Engelm.) and its cultivar ‘Glauca’. The degree and extent of antifungal activity for essential oils listed above were tested on the following fungi: Acremonium tubakii, Anthostomella conorum, Arthrinium state of Apiospora montagnei, Aureobasidium pullulans, Botryodiplodia rubi, Fusarium camptoceras, F. moniliforme var. lactis, Penicillium canescens, Phoma pomorum, Rhizosphaera kalkhoffii, Ulocladium consortiale and Zythiostroma pinastri. Among essential oils tested the thyme oil showed the highest antifungal activity to all fungi under examination even at the lowest concentration used. The mint, lavender and spruce oils demonstrated a medium activity. The lowest activity was found for orange oil.
3
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From studies on possibility of protecting blue spruce [Picea pungens Engelm.] against fungi. Part I. Laboratory assessment of antifungal activity of selected fungicides

84%
Mirski W.
Acta Scientiarum Polonorum. Hortorum Cultus
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2007
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tom 06
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nr 4
The antifungal activity of two fungicides, i.e. Rovral Flo 255 SC and Sportak Alpha 380 EC was determined by in vitro tests to 12 fungal species isolated from blue spruce (Picea pungens) and its cultivar Glauca. It was found that Sportak Alpha 380 EC has the highest antifungal activity and the widest range of application to all fungi under examination. Rovral Flo 255 SC has shown significantly poorer activity.
4
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Fungi occurring on Colorado blue spruce [Picea pungens Engelm.] in the Cracow Botanic Garden

84%
Bartynska M., Mirski W.
Acta Scientiarum Polonorum. Hortorum Cultus
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2005
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tom 04
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nr 2
The aim of the study was to determine the causes of dying down of sprout and needles of Colorado blue spruce (Picea pungens Engelm.) and its cultivar ‘Glauca’ in the park collection of the Botanic Garden of Jagiellonian University in Cracow. For several years, the disease symptoms described in the article have led to the loss of decorative values of these trees. The study was conducted in the years 2002 and 2003 by means of the control of plant healthfulness, phytopathological diagnostics, isolation of microorganisms from tissues exhibiting diseases symptoms, as well as the culture and identification of the isolated fungi. Both, the control of tree healthfulness, and specialized laboratory techniques proved that the symptoms diagnosed in plants had an infectious character, and that the fungi might be the main causing agents. The analysis of the fungal populations isolated from the affected tissues indicates the combined occurrence of fungi in tissues of the studied plants and the domination of the following species: Alternaria consortiale, Aureobasidium pullulans, Penicillium canescens, Phoma pomorum, Ulocladium consortiale, Zythiostroma pinastri.
5
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Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies

67%
Hazubska T., Szczygiel K.
Dendrobiology
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2003
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tom 50
The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.
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