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The experiment was carried out on 936 slow-growing chickens from the crossbreed of Cobb cock with Greenleg Partridge hen. Chicks were randomly divided into two groups: controlled group (BW) with no access to the field and experimental group (W) with access to grass fields since their fourth week of life. In blood serum overall cholesterol, lipoproteins with high density (HDL), triacylglycerols (TG) level was marked. The concentration of lipoproteins with low density was calculated while using the Friedewald et al. formula (1972). No statistically significant influence of sex and system of breeding on cholesterol and its fractions level in blood serum of crossbreed chickens was observed.
A total of 215 animals of twelve inbred strains of mice (A.CA/W, AKR/W, BALB/cW, BN/aW,C57BL/6W, C57Bl/10W, CBA/W, CBAT6/W, C3H/W, DBA/2W, 129Sl/SvW and HLB/219J) bred at the Cancer Centre and Institute of Oncology, Warsaw (Oncology Centre), were characterized In terms of five blood serum biochemical indicators related to their health, reproduction and genetic background. Glucose, total protein, creatinine, triglycerides (triacylglycerols) and total cholesterol were determined in mice aged four months. Significant interstrain differences were found among nearly all parameters considered, with the exception of total protein. The level of triglycerides showed wide interstrain differences and revealed a similar variation trend as the total cholesterol.For cholesterol and triglycerides, significant differences between sexes for the majority of strains were found. The results obtained may be important in the selection of experimental animals,for analysis of changes associated with various diseases, and for the explanation of their genetic background.
The aim of this work was to trace differences in magnesium and calcium concentrations (both total and ionic form) between goats which were in late pregnancy and lactating goats. The study involved 30 goats. Blood samples were taken three times from each goat. Total concentrations of magnesium and calcium were determined with the colorimetric method and (A — 1.581 mmol dm-3; B — 2.052 mmol dm-3; C — 2.112 mmol dm-3). Ionic form of calcium based on using ion—selective analysis (A — 1.219 mmol dm-3; B — 1.126 mmol dm-3; C — 1.123 mmol dm-3). The study showed that mean value of both the total of calcium from each goat did not reach the reference level for this species. Ionic form of calcium was within the lever limit of the physiological norm. The content of magnesium in all the groups was within the range of reference concentrations (A — 1.051 mmol dm-3; B — 1.165 mmol dm-3; C — 1.117 mmol dm-3).
Amifostine is one of the cytoprotective drugs used during anticancer therapy. Amifostine as a thiol compound possesses antioxidant properties and protects only healthy cells against damage, mainly by scavenging reactivity oxygen species, competing with oxygen to prevent oxygen radical interactions with DNA, and promoting cell repair through hydrogen donation to reactive oxygen species. The aim of the present study was to evaluate antioxidative ability of amifostine in blood serum of rats exposed to cyclophosphamide during two weeks after drug administration. We show that amifostine only to a small degree prevents disorganisation of antioxidant systems of blood serum of rats caused by cyclophosphamide action. It is probably connected with low concentrations of amifostine active metabolites in the serum.
Manganese is a component and cofactor for many important enzymes. In blood Mn is complexed to transferrin, and it quickly passes through the body to be extracted mainly in the bile and urine. Almost all Mn pool in blood is located in erythrocytes. Content of manganese in serum, erythrocytes, and hair of 26 men, workers of an airport, was determined. The control group consisted of administrative workers and the test group was composed of airplane servicemen. Hair samples of 0.5 g and 3-4 cm in length measured from the scalp were taken from some places on a head, washed with a detergent solution, rinsed with deionized water, acetone, and dried. Samples of blood were spun. All the samples were mineralized in a mixture of spectrally clean acids HNO3 and HClO4. Concentration of Mn was analyzed by electrothermal atomic absorption spectrometry GFAAS. The concentrations of Mn in the samples of erythrocytes were statistical significantly higher in the test group. In samples of hair, Mn concentrations were comparable between both groups of men. The coefficients of correlation between Mn concentrations in serum and hair, erythrocytes and hair, and between serum and erythrocytes did not imply significant correlations between Mn concentration in the analyzed clinical samples. In contrast, in the erythrocytes of men exposed on aviation fuel the content of Mn was significantly higher.
The activity of monoamine oxidase (MAO) in cow serum undergoes considerable fluctuations in the course of the oestrous cycle. The aim of the study was to determine the activity of MAO and its isoenzymes A (MAO-A) and B (MAO-B) in cow serum near the parturition. The experiments were performed on 8 pregnant and clinically healthy cows of the Polish Black and White Breed. Peripheral blood samples were collected every 4 hours before, during and after parturition. MAO serum activity was determined with the use of the spectrofluorimetric technique according to Matsumoto et al. (1985) with own modifications. Analysis of serum samples showed that MAO activity increased rapidly near the delivery. MAO-B activity was significantly higher than MAO-A activity and determined the profile of total MAO (MAO-T) activity. It may be suggested that an increase in MAO activity observed near delivery protects against an excessive elevation of catecholamine concentration in blood. However, the control mechanism and physiological role of MAO during this period need further investigations.
The study was performed on pregnant ewes and their progeny. The experimental group of ewes was given injections of 5 mg selenium in the form of sodium selenite. The sodium selenite s.c. injections resulted in monre than a triple, significant increase in the selenium level in ewes, at the first month as compared to the initial concentration of 0.42±0.11 to 1.38±0.21 μmol/l. Also in lambs a marked increase in selenium was noticed in the first and fifth weeks of life, but the levels of this element were by a half lower than in their mothers. Moreover, considerable diminution of magnesium concentration was observed in both groups: from 1.05±0.05 to 0.82±0.07 mmol/l in Group 1 of ewes in the second month following Na₂SeO₃ injection, and from 0.94±0.05 to 0.82±0.08 mmol/l in lambs, in the ninth week.
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