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Nigella sativa is an annual flowering plant, native to Iran and other parts of southwest Asia, belonging to the buttercup family (Ranunculaceae). Its grain has several important health-related properties such as anti-parasitic, anti-virus, anti-bacterial, increased milk production, carminative, and anti-diabetic. To determine effects of fertilizer treatments on this plant, this experiment was conducted in randomized complete block design in three replications with seven treatments. Fertilizer treatments consisted of Bio phosphor (B 2 kg∙ha-1), Bio sulphur (S 5 kg∙ha-1), Biological fertilized phosphate BAVAR 2 (F 100 g∙ha-1), chemical fertilizer (M) according to soil analysis, and combined fertilizer (B+S), (S+F), and (C) treatment. The application of fertilizers showed a significant effect (p<0.01) on plant height, number of capsules per plant, seed weight, grain yield (g/m-2), number of flowers per plant, and biological yield (g/m-2). There were no observed significant differences (p<0.01) on the number of seeds per capsule, seed weight and yield between chemical fertilizer and biological BARVAR 2. Thus, it can be concluded that there is no preventative for biological fertilizer to produce a good yield of biological product for human health.
The aim of this study was to investigate the potential antioxidant activity of black cumin seeds as a dietary supplement. Three hundred 3-day-old broiler chicks were divided randomly into five treatment groups (control, and black cumin at four levels) of 60 birds each. Black cumin seeds at 0.5%, 1%, 2% or 3% were added to the basal diet and their effects determined on malondialdehyde (MDA) and vitamin E levels in the serum, breast muscles, liver and heart muscles in the 42-day old broilers. When the diet was supplemented with 2% and 3% black cumin seed MDA concentration in serum (p < 0.05), breast muscle (p < 0.01), liver (p < 0.05) and heart muscle (p < 0.05) was significantly more reduced compared to birds fed 1% and 0.5% black cumin seeds and control diet. Supplementing the diet with black cumin seeds did not significantly affect vitamin E levels in serum and tissues. These results suggest that black cumin seeds could be considered as a natural potential antioxidant promoter for poultry, and showed the best responses at 2% and 3% level of inclusion.
Extracts of dried leaves of dill (Anethum graveolens) and parsley (Petroselinum crispum), black pepper fruits (Piper nigrum), and black cumin seeds (Nigella sativa) under and after simulat­ed digestion were investigated for their antiradical activity, iron chelation, iron reduction and inhibition of lipid peroxidation. The changes of total phenolic compounds, phenolic acids, quercetin and kaempferol content were also measured. In vitro digestion caused significant increase of total phenolics content in all samples, except black pepper fruits extract. All samples showed low ability to scavenge DPPH radicals (below 18%). The highest activity was observed for parsley leaves extract, and the lowest in the case of black pepper fruits extract. Hydrolyzed extracts from black cumin seeds, parsley and dill leaves showed significantly better iron chelating capacity then non-hydrolyzed samples. After digestion this activity increased in all samples, except black pepper fruits extract. The highest ability to inhibition of lipid peroxidation was obtained for hydrolyzed black cumin seeds samples. Activity of all samples increased after digestion.
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