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Phoma negriana is a little-known pathogen of grapevine canes but increasingly often it is listed in the countries of southern Europe and Asia. The work concerns a study of the opportunities for growth and development of the fungus at different temperatures, pathogenicity tests and the influence of biotechnical preparations on the growth and development of the fungus in vitro. Studies have shown that mycelial growth of P. negriana is possible at the temperature range from 5 to 32ºC with the thermal optimum from 20 to 24ºC. The optimal temperatures for the abundant sporulation was 20, 24 and 28ºC. The positive results of pathogenicity tests as well as re-isolation of the fungus from inoculated shoots confirmed the possibility of grapevine canes infection by P. negriana. A significant factor in facilitating infection were injuries of shoots, what indicates that the fungus is a facultative pathogen of grapevine. Biosept Active limited the growth and development of P. negriana significantly more strongly than Beta-Chikol. When it was applied at the concentration of 0.3%, it caused destruction and loss of fungal hyphae vitality.
The aim of the study was to determine the effect of various biopreparations on the growth of the apple root system, the number of spores of AMF, the total number of bacteria and microscopic fungi in the rhizosphere soil, and the degree of mycorrhizal association in the roots of two apple tree cultivars. The experiment was established in the spring of 2009 in the Experimental Orchard of the Institute of Horticulture in Dąbrowice. The research objects were one-year-old maidens of the apple cultivars ‘Topaz’ and ‘Ariva’ grafted on M26 rootstock. The trees were treated with the following biopreparations: control, control NPK (standard NPK fertilization), manure, Micosat F + manure, Humus UP, Humus Active + Aktywit PM, BioFeed Amin + manure, BioFeed Quality + manure, Tytanit + manure, Vinassa + manure, Florovit Eko, and Florovit Pro Natura. Treatment of ‘Topaz’ and ‘Ariva’ apple trees with the biopreparations Micosat F, Humus UP, Humus Active + Aktywit PM, BioFeed Amin, Vinassa, Florovit Eko and Florovit Pro Natura produced positive effects on the growth of apple roots and their mycorrhizal frequency, and the size of the populations of microorganisms in the rhizosphere soil.
Striving to intensify horticultural production, new and more effective bio-preparations are being sought to stimulate plant growth and development. Bio-algeen S90 is a natural agent based on sea algae, the high biological activity of which results from the high content of natural growth regulators. The aim of the study was to verify the influence of Bio-algeen S90 on the growth, morphological characteristics and chlorophyll fluorescence of Rosa multiflora seedlings. The bio-preparation was applied one, two and three times at concentrations: 0.1, 0.2, 0.4 and 0.6 mg.dm−3. Following parameters were measured to evaluate the response of plants to the bio-preparation: F0 – initial fluorescence, Fm – maximal fluorescence in the dark-adapted state, Fv/Fm – maximum photochemical efficiency of PSII. All concentrations of the bio-preparation and frequency of its application stimulated the number of shoots in a bush, the length of shoots and the diameter of the root crown of plants intended for budding. The most beneficial was the two-fold bio-preparation application at a concentration of 0.4 mg.dm–3. Bio-algeen also positively influenced the chlorophyll fluorescence parameters. The highest mean F0 and Fm values were recorded with the two-fold preparation treatment. There was no significant effect of the bio-preparation on the Fv/Fm index, which was within the range of 0.75–0.66.
Influence of triforine (standard), garlic juice, Antifung 20 SL (20% vermicompost), Atpolan 80 EC (76% mineral oil), Biosept (grapefruit juice) and Atonik AL on development of Sphaerotheca pannosa var. rosae was evaluated. Garlic juice, Atpolan 80 EC and Biosept applied as rose spray gave similar effect in the control S. pannosa var. rosae as triforine. Observations under scanning electron microscop indicated that 24 hrs after rose spraying all tested compounds cause almost complete collapse of conidia and hyphe.
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