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A recent paper on the occurrence of the genus Crocidura in Tunisia reports a single specimen identified as C. suaveolens. Therefore a third species, besides C. russula and C. whitakeri would occur in the country. However, the presence of C. suaveolens in North-Africa is controversial and was recently ruled out from the other Maghrebi countries (Algieria and Morocco). During the period 1989 - 90, 71 specimens of shrews were collected from owls pellets or trapped at Tunisian 12 sites. This material was measured and studied both by classic morphometric and multivariate methods (Fuzzy test, Principal Coordinate Analysis and Generalized Procrustes Analysis), considering also reference samples (C. suaveolens from Italy and Balearic Islands, C. russula and C. whitakeri from Morocco). Four of the 12 trapped shrews were karyotyped and resulted to have a C. russula karyotype, some differences in biometry as well as in Number of Fundamental arms (NFa) separate the Moroccan population from the Tunisian one and raise some interesting questions on the taxonomy of C. russula. Multivariate analysis also allowed us to exclude the presence of C. suaveolens from the Tunisian material studied and suggested also the misclassification of the proposed specimen that should be reconsidered as C. whitakeri. A preliminar distribution map of the two species living in Tunisia was drawn, also based on the scanty data from the literature.
The objective of this study was to investigate the ranges of intra- and interindividual variability on the example of R. canina. For this purpose, four flowers were collected randomly (72 flowers in total)from 18 wild shrubs of R. canina growing in one population in Poznań (Poland)and then, from each flower, 50 correctly formed pollen grains (200 pollen grains per each individual)were selected. Inter- and intraindividual pollen grain variability was characterised based on 3600 pollen grains. They were analysed for seven quantitative features, i.e. length of polar axis (P), equatorial diameter (E), thickness of the exine along the polar axis (Exp), length of ectocolpi (Le) and P/E, Exp/P, and Le/P ratios. Our study revealed highly significant differences among flowers of the particular R. canina individuals with respect to all pollen grain biometrical features. In addition, it showed that the assessment of the full range of variability in pollen grain biometric features within one individual (shrub)was more reliable if we examined several pollen grains from several flowers than for the same number of pollen grains derived from a single flower. We also found statistically significant differences among particular individuals in all pollen grain features. This proves that in order to well characterise a population of a given species from the point of view of palynology, the plant material should derive from a possibly numerous number of individuals (shrubs).
The characteristic of Pinus uncinata on the basis of biometrical analyses of 16 cone features was done. The research was based on 8 samples representing 6 populations, consisting of 50 cones each, collected in the Pyrenees, within the natural range of the species. All the investigated cone features were moderately differentiated, with the variation coefficient ranging from 8 to 30%. The particular populations differ significantly from each other statistically according to several features examined. The samples collected in the same population also vary significantly. The differences between populations, however, do not enable their division into groups, which supports their origination from the same Pleistocene refugia.
The study concerns the ecological responses of Tilia cordata Mill. to pollution and describes the advantages of estimating of the degree of environmental pollution. The aim was, to find out whether the degree of pollution conforms to the contents of the investigated elements in leaves, the assessment of variability of morphometric leaf traits and the cationic equilibrium values. The study has shown a statistically significant differentiation between the analysed sites in respect of dust fall, concentrations of elements in soils and leaves, morphometric traits of leaves and the cationic equilibrium. The hierarchical tree plot of cluster analysis qualified the sites as polluted and non-polluted. In polluted sites the contents of metals in leaves were significantly higher and the cationic equilibrium in leaves was disturbed. The surface, width and asymmetry of leaves, and also the variability of these measures were higher in polluted sites. In sites, which are not directly affected by pollution the cationic equilibrium is close to the const. value, whereas leaf traits have shown the lowest values and a considerably lower variability. The investigations show that leaf traits and cationic equilibrium correspond to the level of pollution and can be used as a tool for evaluation of the environment status.
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