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Ellagic acid is a biologically active compound, regarded as preventive against various diseases and demonstrates anticarcinogenic and antioxidant effects. In Poland, one of the five largest strawberry producers, the supply of these fruits is significant; therefore exploration of this subject and dissemination of the knowledge concerning their nutritional values and health-related properties is important. The aim of the study was to determine the content of ellagic acid in the most popular, selected strawberry cultivars in Poland. Fruits of the strawberry cultivars under examination differed significantly in ellagic acid content. The content of the analysed component ranged from 452.5 mg kg⁻¹ fresh weight (4,575.6 mg kg⁻¹ dry matter) in fruits of the ‘Heros’ cultivar to 1,193.8 mg kg⁻¹ fresh weight (14,215.0 mg kg⁻¹ d.m.) in fruits of the ‘Camarosa’ cultivar. The highly diversified content of ellagic acid in fruits of individual cultivars, grown in similar soil and weather conditions and subjected to the same agricultural treatments, indicates that the content of the analysed phenolic acid depends not only on the fruit species, but also on the cultivar.
Traditionally, a drug is expected to be biologically active and at the same time be able to ensure some sort of tissue or organ specificity. The latter property is necessary to avoid undesirable side effects when toxic drugs are being used. Such requirements are difficult to achieve only by changing the chemical formula of the drug. For these reasons, within the last few years a new pharmacological concept has been developed regarding delivery of biologically active compounds by the use of macromolecular aggregates. The purpose-specific design of macromolecular aggregates, able to deliver drugs to a desired location, is based on the assumption that different functions can be assigned to the separate chemical entities forming the aggregate. With the help of such an aggregate, the biologically active compound can be designed with solely its pharmacological potency in mind and without considering any limitations imposed by inaccurate delivery, such as undesired side effects. Specific molecules of the aggregate would ensure desired compound distribution within the organism. Furthermore, other molecules forming the aggregate should fulfill additional functions, e.g. protecting the drug from degradation. Additionally, aggregates formed from amphiphilic molecules should be capable of carrying drugs that are difficult to use as therapeutic agents due to low solubility in biological fluids (e.g. Taxol) or degradation (e.g. peptides, DNA). Such aggregates can be constructed from natural or/and synthetic compounds. Taken together, this creates possibilities of extending the spectrum of drug application and allows for the introduction of new technological modifications.
The main objective of the present study was to investigate the effect of spray- and freeze-drying techniques on the microencapsulation of a gallic acid compound using the acid-hydrolyzed low dextrose equivalent potato starch as a wall material. During the experiment, it was possible to achieve encapsulation efficiency of 70–84% for the freeze-dried and 65–79% for spray-dried samples, without statistically signifi cant difference (P>0.05) in the encapsulation efficiency between the mentioned methods. Spray-dried samples formed spherical capsules with a higher number of micropores. Meanwhile, freeze-dried samples were shapeless, exposed larger pore volume (from 2.4×10–3 to 9.5×10–3 cm3 /g against 1.2×10–3 4.9×10–3 cm3 /g; analyzed by Barrett-Joyner-Halenda method) and overall higher surface area (0.632–1.225 m²/g against 0.472–1.296 m²/g; analyzed by Barrett-Joyner- -Halenda method). Due to this fact, more gallic acid molecules were exposed to environmental factors and can be counted as losses. In addition, freeze-dried samples demonstrated lower water activity than spray-dried samples (0.075±0.014 against 0.178±0.008). Results showed that it is not practical to use freeze-drying for modelling encapsulation for food industry without a special necessity for protection of easily degradable chemical compounds. The present work makes a basis for the future studies of the microencapsulated phenolics application in food production.
The phenomenon of chirality, common in nature, pertains also to food components. The effect of the chiral structure of chemical compounds is the occurrence of optical isomers, i.e. enantiomers. The only source of optically active substances are living organisms, which produce most frequently mostly one of isomers. Proteins and carbohydrates are chiral: amino acids are found in proteins only in the L form, whereas carbohydrates are formed of D-saccharides. Enantiomers may exhibit diverse behaviour in reactions with biologically active compounds of living organisms. Chiral structure of proteins turned out to be significant in the perception of taste perceptions as well as the perception of aroma. The most characteristic phenomenon is chirality among odorants. Numerous compounds essential for aroma are found in nature in the form of two isomers, with considerable predominance of one of them, while the ratio of these isomers is specific and stable. Enantiomers of odorants may differ in the intensity and character of their smell. The phenomenon of chirality has been known since mid-1800; however, it then constituted a serious analytical problem. Only the development of methods facilitating the separation of optical isomers created a possibility to investigate its universality and importance in living organisms. The application of more and more advanced analytical techniques in the control of chiral nature of food ingredients may be used to detect adulterations and especially to determine authenticity of fragrances.
Studies on contents of phenolic compounds and diterpenes in Salvia miltiorrhiza Bunge root during the vegetation period were carried out. In Polish climate and soil condition, the tanshinone II A occurred as the dominant compound from diterpenes group. Obtained results show that the most profitable period for harvesting Salvia miltiorrhiza raw material is fructification stage. However, it is very difficult to decide on the accurate harvest date in this period. After all, taking into account various weather conditions, harvest of the Salvia mitiorrhiza Bunge should be done at the end of the vegetation period (in November).
Coumarins are very common biologically active compounds that have been found in nu­merous species of the family Apiaceae. The biological and pharmacological activity of these substances has very interesting properties. Coumarins also play a major role in the plant defence system. The quality and quantity of furano- and piranocoumarins is important for a defensive role in a plant as these compounds form a barier between the environment and plant organs. In order to study the plant defence mechanism it is necessary to carry out a qualitative and quantitative analysis of both furano- and pyranocoumarins. In this paper the studies on the optimum extraction and their influence on the yield of furano- and pyranocoumarins from fruits of the species Peucedanum verticillare are reported. The coumarins were analysed quantitatively by a combined method of SPE and RP-HPLC.
The research of mineral fertilization effect of Salvia miltiorrhiza Bunge has been carried out in 2004–2006. It was found that potassium fertilization had the biggest influence on the amount of dry root mass changeability. The evaluation of mineral fertilizer effect of biological active compounds gave ambiguous results. In the raw material of Polish cultivation origin the phenolic acids and tanshinones, which determine the healing properties of Salvia miltiorrhiza, were noticed. In the roots the tanshinone IIA occurred to be the dominating substance from diterpenes group. The weather conditions significantly influenced the amount of biological active compounds.
This paper is a review of studies on the interaction between cationic, amphiphilic quaternary ammonium salts and biological and model membranes. It has been pointed out that the compounds studied induce changes in both passive and active membrane properties such as mechanical, transport liquid-crystalline and electrical . An attempt has been made to explain the observed effects on the basis of the molecular shape and hydrophobic matching concepts. Some practical implications of the observations and their interpretation have also been noted.
Four populations of roseroot (Rhodiola rosea and Rh. quadrifida) originating from Poland and Mongolia were compared in respect of the content of main biologically active phenolic compounds (salidroside, p-tyrosol, trans-cinnamic alcohol, rosarin, rosavin and rosin) in the underground organs. Three solvents (75% ethanol, methanol and water) were used for the extraction of these phenolic compounds. Rh. rosea grown in Poland was characterised by similar content of salidroside and higher content of rosavin in comparison to the plants growing wild in Mongolia. The content of these compounds in Rh. quadrifida was lower than in Rh. rosea. Water appeared to be the best extraction medium for the isolation of salidroside and tyrosol. Cinnamic alcohol and its derivatives were more efficiently extracted with methanol and 75% ethanol.
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