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The aim of this study was to assess the possibility of using biological and biochemical parameters in the evaluation of forest soil quality and changes caused by land use. The study attempted to determine a relationship between the enzymatic activity of soil, the number of earthworms and soil physico-chemical properties. The study was carried out in central Poland in adjoining Forest Districts (Przedbórz and Smardzewice). In soil samples taken from 12 research plots, basic physico-chemical properties, enzyme activity (dehydrogenase, urease) and density and biomass of earthworms were examined. Enzyme activity showed a large diversity within the forest site types studied. The correlations between the activity of the enzymes studied and C/N ratio indicated considerable importance of these enzymes in metabolism of essential elements of organic matter of forest soils. Urease and dehydrogenase activity and earthworm number showed susceptibility to soil pH, which confirmed relationships between enzyme activity and abundance of earthworms and soil pH in H2O and KCl.
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Experimental studies as an inspiration for clinical investigation

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This study represents an attempt of showing own author's example of using basic research data as an inspiration for the clinical studies. The project evaluates the role of gastrin in colorectal carcinogenesis as well as the differences of its action in proximal and distal colon. Colonocytes were isolated from Fischer-344 rats and incubated for 2 minutes with gastrin (10-8M). This treatment resulted with 60-70% rise in tyrosine kinase (Tyr-k) and 150-200% - in phospholipase C activity as regards to basal levels. In vivo infusion of gastrin for 5 days to Fischer-344 rats resulted with 90-150% increase in distal but not proximal colonic mucosal proliferative activity as well as tyrosine phosphorylation of several colonic mucosal proteins. In clinical study, the mean fasting gastrin level in the control group was significantly lower (p<0.01) than in patients with colorectal cancer before surgery. Mean plasma gastrin level in patients with distal tumor yielded 105,31 ± 12,5 µ U/l and was significantly higher than in patients with the proximal tumor site (42, 2 ± 3,1 µU/l) (p<0,001). We conclude, that Tyr-k is involved in the mechanism of the trophic action of gastrin, particularly in distal colon. The differences in gastrin concentration in patients with distal and proximal tumors may probably contribute to the distinct pathogenesis and biological properties of those cancers.
Several chimeric peptides consisting of the N-terminal fragment of galanin (GAL) and C-terminal fragments of other bioactive peptides (e.g. substance P, bradykinin, neuropeptide Y, mastoparan) have been synthesized and reported as high-affinity galanin receptor antagonists. Recently we have synthesized a new chimeric peptide, GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2, consisting of the N-terminal fragment of GAL and the C-terminal fragment of endothelin-1 (ET-1) analogue. This chimera was previously shown to be a moderate-affinity ligand to hypothalamic galanin receptors with a KD value of 205 nM. However, its biological action has been unknown so far. In our studies we characterized the biological properties of this new chimeric analogue, investigating its action on rat isolated gastric smooth muscles and influence on insulin secretion from rat isolated islets of Langerhans. Data acquired in the course of our studies suggest that analogue GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2 does not seem to be a potent galanin receptor antagonist in the gastrointestinal tract.
The objective of the study has been to determine the effect of the herbicide Apyros 75 WG on counts of various aerobic microorganisms, activity of soil enzymes and yields of spring wheat. For this purpose, a pot experiment was carried out in a greenhouse. Samples of soil used for the trials represented loamy sand. Having mixed the soil samples with mineral fertilizers, doses of the herbicide were added and the soil was placed in plastic pots. The lowest herbicide dose was the optimum dose recommended by the producer, and the two other doses were 10- and 100-fold higher. The experiment was conducted in two series: I – unsown soil, and II – soil under spring wheat. It has been determined that Apyros 75 WG disturbs soil’s homeostasis, as it disrupts multiplication of some microbial groups, inhibits the activity of soil enzymes and depresses the yield of spring wheat, even if applied in a recommended dose. Among the soil enzymes, dehydrogenases and urease were the least tolerant to the effect of the herbicide, whereas alkaline phosphatase proved to be the most tolerant one. The vulnerability of microorganisms to soil pollution with the herbicide can be arranged in the following decreasing order: ammonifying bacteria > Pseudomonas > copiotrophic bacteria > oligotrophic bacteria > nitrogen binding bacteria > spore-forming oligotrophic bacteria > Arthrobacter > cellulolytic bacteria > Actinomyces > fungi. Growing spring wheat had a positive effect on the counts of microorganisms and activity of soil enzymes.
For sustainable soil use soil quality is essential presumption. Soil quality does not depend only on the physical and chemical properties of soil but it is very closely linked with the biological properties of soil mostly on microbiological processes. Soil quality is a dynamic feature, and any significant indicators must be sensitive to small changes in key soil properties. During the years 1991-2002 soil samples were collected at four Cambisol localities (totally 184 samples) and five Luvisol localities (totally 230 samples). Chemical (soil organic carbon [Corg], total nitrogen [Nt], pH KCl), textural (content of sand, silt, clay particles) and biological characteristics (microbial biomass carbon [Cmb], K2SO4 extractable microbial carbon [Cex]), respiration, ammonification, nitrification) were studied. The higher level of Corg (1.20-1.76%) characterised Cambisols and resulted in quite high microbial biomass carbon content (396—625 ug/g dry soil, average 556, SD 167). For these soils high control respiration (0.45-0.80 mg C02/h/100 g dry soil) and potential nitrification with (NH4)2S04 (6.7-18.4 mg N-NO3/8 days/100 g dry soil) were typical. Studied Luvisols reached lower levels: Corg (0.97-1.22%), Cmb (398-503 eg/g dry soil, average 455, SD 98), control respiration (0.46-0.57 mg C02/h/100 g dry soil), potential nitrification with (NH2)4SO4 (3.2-9.9 mg N-NO3/8 days/100 g dry soil). Mentioned lower level of organic carbon and medium level of microbial biomass raised in higher ratio Cmb/Corg (average 4.0%).
In the course of food proteins digestion, mid-molecular weight opioid peptides are generated. Their amine groups might undergo non-enzymatic glycation with glucose which is promoted by pH and temperature. The glycation conditions of morphiceptin (pH 7.8 and temp. 37°C) used in this experiment proved that non-enzymatic glycation of the peptides could take place in the digestive tract. The results obtained herein suggest that glycation decreases the contraction amplitude of isolated rabbit duodenum by 14.1% and changes the immunogenicity of morphiceptin (Tyr-Pro- Phe-Pro-NH2), probably due to a new epitope produced.
Adoption of sustainable tillage can protect soils from biological degradation and maintain soil quality, as compared with conventional management. This paper presents findings from a long-term tillage experiment carried out in Endocalcari-Endohypogleyic Cambisols on a sandy loam soil in Lithuania. The tillage systems were: conventional (CT), moderate (MT), and no tillage (NT). Tillage intensity positively affected microbial substrate utilization and urease activity, as well as, negatively, dehydrogenase activity, bacteria and fungi amounts, and Shannon diversity index of microbiological community. Higher total porosity provoked higher enzyme activity; but, microbial activity correlated negatively with bulk density.
На пахотной песчаной аллювиальной почве опытной станции своец проводились химические и биологические исследования почвы используемой в течение 25 лет в качестве поля севооборота, черного пара и природной залежи. В сравнении с почвой поля севооборота в почве черного пара уменьшилось на около 30% содержание органического С, общего N, P₂O₅ и K₂O, а в почве залехи содержание органического С на 68%, N на 54% и СаО на 30%, при одновременном четком снижении содержания P₂O₅ и K₂O. В составе гумуса увеличилось участие фульвовых и гуминовых кислот, а уменьшилось содержание гумина в почве черного пара. В почве залежи направление изменений было обратным. Активность сахаразы и каталазы была самой сильной в почве залежи, а гораздо слабее в почве под севооборотом и черным паром. Густота Collembola и Acarina были в почве залежи двухкратно выше, чем в почве поля севооборота, а в почве черного пара наполовину меньше.
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