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The aim of this work was to explore the possibilities of utilising the macroalgae accumulating on the Sopot beach, a part of the Gulf of Gdańsk (Baltic Sea). During this work (2004–2006) a range of activities were undertaken: monitoring the occurrence of macroalgae, collection of plant material, taxonomic identification, laboratory analyses of contamination (metals – Cd, Pb, Ni, Zn, Cu, Cr, Hg; radionuclides – 137Cs, 40K; organic contaminants – polychlorinated biphenyls, polycyclic aromatic hydrocarbons), the stage of algal degradation and studying possible uses of the plant material collected on the beach. The most suitable way of utilising the plant material seems to be as a biofertiliser. Laboratory growth tests were carried out to assess this possible use; they were successful.
Biofertilizers are the special formulation of specific beneficial microorganisms that promote the growth of plant crops by converting the unavailable form of nutrients into available form. Here, the effect of heavy metal stress on antioxidant enzymes were studied in Clitoria ternatea L. leaves. Clitoria ternatea L. plant was grown for 30 days and the heavy metal mercuric chloride was sprayed after 10 days from the date of planting. Effect of mercuric chloride was observed in treated plants. The selected plant Clitoria ternatea L. was grown under mercuric chloride treatment in a specified concentration 1 μg/10 ml. The control plant maintained without the treatment of mercuric chloride. Antioxidant effect of mercuric chloride was measured under controlled and treated conditions. The selected plant Clitoria ternatea L. was grown under mercuric chloride in treatment. Further it increases H2O2 content and the antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were observed in mercuric chloride treated plants when compared with control. Here mercuric chloride was accumulated more in matured leaves. The results are discussed with the literature.
Green revolution increased the food production enormously to feed the geometrically growing population. In India, the availability and affordability of fossil fuel based chemical fertilizers at the farm level have been ensured only through imports and subsidies. Dependence on chemical fertilizers for future agricultural growth would mean further loss in soil quality. The possibilities of water contamination and unsustainable burden of the fiscal system. The Government of India has been trying to promote an improved practice involving use of biofertilizers along with fertilizers. These inputs have multiple beneficial impacts on the soil and can be relatively cheap and convenient for use. The agricultural chemicals like pesticide and weedicides were being used to save the standing crop from the attack of pests and to boost crop production. At the same time the excess use of chemical fertilizers degraded the soil properties. In this context, the farmers are expecting for the cheapest and eco-friendly alternative for getting higher yield. Biofertilizers is only the alternative way to get more production without harmful to environment. So the effect of different doses of biofertilizer (Rhizobium) on germination studies of Fenu-greek seed germination percentage, seedling growth, fresh weight and dry weight, chlorophyll, protein and soluble sugar content has been studied in the laboratory condition. There parameters increased to application when biofertilizer compared to control.
In recent years, biofertilisers have emerged as a promising component of an integrated nutrient supply system in agriculture. The objective of this study was to examine the influence of selected biofertilisers on the vegetative growth, the content of N, P2O5 and K2O in the leaves and stems, and on the physiological parameters of pepper of the variety of ‘Sofiiska Kapiya’ cultivated under organic agriculture conditions. This experiment was carried out from 2009 to 2011 on the experimental fields of the Agroecological Centre at the Agricultural University-Plovdiv (Bulgaria), situated on the territory of a certified ecological farm. The study included the following biofertilisers – Boneprot, Lumbrical, Baikal EM “Effective Microorganisms”, Emosan, and Bio One. The results of the biometric measurements of the average plant height at the end of the vegetative period showed the highest values for the variant treated with Emosan on the Boneprot basic fertilisation (62.60 cm – 2009; 64.80 cm – 2010, and 63.87 cm – 2011). Upon feeding with the biofertilisers Emosan and Baikal EM on basic fertilisation with Boneprot (2009, 2010 and 2011) at the pepper mass fruit yield stage, plants showed higher values of net photosynthesis (PN) that were also similar to the high values observed in the flower bud stage. The highest intensity of transpiration (ȿ) was observed for the variants treated with the biofertilisers Baikal EM (2009 and 2011) and Emosan (2010) on basic fertilisation with Boneprot. It was concluded that the feeding with Emosan stimulated the vegetative growth of the pepper plants due to the high concentrations of nutrient-providing proteins contained in this biofertiliser. The results showed that biofertilisers do not significantly impact the P content of the pepper leaves and stems, but changes were more obvious in the leaves. The fertilisation with the studied biofertilisers increased the K2O content in leaves and stems compared to the control (non-fertilised) plants; the values were higher for the leaves.
Nigella sativa is an annual flowering plant, native to Iran and other parts of southwest Asia, belonging to the buttercup family (Ranunculaceae). Its grain has several important health-related properties such as anti-parasitic, anti-virus, anti-bacterial, increased milk production, carminative, and anti-diabetic. To determine effects of fertilizer treatments on this plant, this experiment was conducted in randomized complete block design in three replications with seven treatments. Fertilizer treatments consisted of Bio phosphor (B 2 kg∙ha-1), Bio sulphur (S 5 kg∙ha-1), Biological fertilized phosphate BAVAR 2 (F 100 g∙ha-1), chemical fertilizer (M) according to soil analysis, and combined fertilizer (B+S), (S+F), and (C) treatment. The application of fertilizers showed a significant effect (p<0.01) on plant height, number of capsules per plant, seed weight, grain yield (g/m-2), number of flowers per plant, and biological yield (g/m-2). There were no observed significant differences (p<0.01) on the number of seeds per capsule, seed weight and yield between chemical fertilizer and biological BARVAR 2. Thus, it can be concluded that there is no preventative for biological fertilizer to produce a good yield of biological product for human health.
The aim of the present study was to investigate the influence of different bioferilizers on the yield, fruit quality and storage properties of ‘Sampion’ apples. Foliar sprays of AlgaminoPlant and Goëmar BM 86 were conducted once or three times, whereas HumiPlant was used for ground spraying. An assessment of the total yield, mean weight of fruit, fruit firmness (FF), soluble solids content (SSC), titratable acidity (TA) was performed at harvest as well as after the storage period (150 days). The obtained data indicate an overall beneficial effect on the yield and its quality. Foliar sprays increased the total yield of the investigated apple trees. Goëmar BM 86 and HumiPlant significantly enhanced the mean fruit weight as compared to the control. Apples treated with Goëmar BM 86 showed the highest fruit firmness and soluble solids content, however titratable acidity was at the lowest level. Treated apples revealed a lower organic acids reduction after the storage.
Biofertilizers are becoming increasingly popular in many countries and for many crops, but very few studies on their effect on growth in seedling stage have been conducted in rice. The experiments were conducted under laboratory conditions due to organic manure, fertilizers and bio fertilizers were observed in all experiments. The treatments such as organic manure, chemical fertilizers and Biofertilizers alone and in combination were applied. The growth parameters viz. germination percentage, root length, shoot length, fresh weight and dry weight were measured. Similarly the pigment contents such as chlorophyll-a, b, total chlorophyll and carotenoid were also measured at seventh day after sowing. All the parameters were higher in combined application of Biofertilizers than in single application and other treatments including control.
The field experiment was conducted in split design with three replicates in Arunagirimangalam village, Thiruvanamalai District to study the effect of groundnut haulm compost of single and combined inoculation of FYM, different biofertilizers (Azotobacter, Azospirillum, Phosphobacteria, Rhizobium) on growth, photosynthetic pigments, biochemical properties and yield of Arachis hypogeae L. The results of this experiment revealed that groundnut haulm with combined inoculation of biofertilizers significantly increased growth, photosynthetic pigments, biochemical and yield parameters of the test crop. Among various combinations, single inoculums of groundnut haulm performed better than control while groundnut haulm with combined inoculation of Rhizobium found better over single inoculation treatments. In general, it could be concluded that groundnut haulm compost and mixed with Rhizobium may be a good tool to improve the crop yield productivity and quantity and soil health.
Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na⁺) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K⁺, and Ca²⁺) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na⁺ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plants to grow well under saline conditions.
The present investigation has been carried out on Tagetes erecta to search out the effect of sugar mill effluent on growth and yield. In-vivo conditions were set up for the experiment purpose. Pots were filled with soil and treated with different concentrations of sugar mill effluent i.e., 10, 25, 50, 75 and 100% on morphological parameters of test crop. Results of pot culture experiment showed that at 10 % concentrations of sugar mill effluent proved to better to the crops growth and thereafter the growth may reduce over control. In order to mitigate the effluent toxicity certain Biofertilizers inoculants were mixed to the soil along with the same effluent treatments. The study suggests that the Biofertilizers inoculants along with effluent irrigation proved to be better for plants growth.
In intensive fruit production, in order to obtain high yields, it is necessary to use high fertilization rates as well as plant protection products. An alternative to that kind of production is presented by natural stimulators of plant growth and development called biofertilizers, biopreparations, biostimulators or phytostimulators. They are prepa­rations of natural (plant or animal) origin, harmless to humans and the environment. Biostimulators contain biologically active substances, i.e. plant hormones, enzymes, macro- and microelements, and other compounds that stimulate the growth and development of plants. New approaches to agriculture tend to use environmentally friendly and safe products with a broad spectrum of activity. Nowadays many preparations offered for crop production are designed not only to fertilize the plants and stimulate their growth, but also to protect them from diseases or pests. Phosphite-containing products act as fertilizers or fungicides, and sometimes as biostimulants. "Resistim" is an activating stimulant and fertilizer composed of a potassium phosphite and natural betaines. Vol. 18(1)2010: 111-124 The aim of the study was to evaluate the influence of "Resistim" on the growth and development of three strawberry cultivars (Fragaria x ananassa Duch.). Two of them are short-day cultivars: 'Honeoye' and 'Elsanta', while 'Selva' is a day-neutral cultivar. The experiment was established in October 2008 under controlled conditions in a glasshouse and was carried out for three months. The plants were planted into a mixture of sandy mineral soil and peat in rhizoboxes enabling visualization of root growth and development. The plants were fertilized with standard NPK fertilization (N - 1.02 g, P - 1.9 g, K - 0.78 g per rhizobox), "Resistim" at the dose of 0.2% and 0.4% and NPK (N - 1.02 g, P - 1.9 g, K - 0.78 g) with "Resistim" at the dose of 0.2% and 0.4%, andwatered by an automatic, computer-controlled watering system. Control plants were not fertilized. The data obtained showed significant differences in the responses of the cultivars to "Resistim" treatment 'Honeoye' was the most responsive, although the other two cultivars also responded positively to the treatment with "Resistim". Further field research is required to determine whether the supplemental application of "Resistim" can be beneficial for strawberry fruit production.
The aim of this study was to examine the effects of management practices and biofertilization on microbial activity in rhizosphere and yield of medicinal and aromatic plants. Field experiment was performed using four plant species: peppermint (Mentha × piperita L.), pot marigold (Calendula officinalis L.), sweet basil (Ocimum basilicum L.), and dill (Anethum graveolens L.). Treatments were arranged in a split-plot layout in four replicates using basic plots under conventional and organic management, and subplots with and without biofertilizer (Azotobacter chroococcum). Organic management positively affected the microbial number and activity. Biofertilization increased the total microbial number (13–21%), number of ammonifiers (13–60%), nitrogen-fixing bacteria (7–36%), actinomycetes (36–50%), fungi (60–100%), cellulolytic microorganisms (57–217%), dehydrogenase (28–52%) and ß-glucosidase activity (15–39%). The effects of management practices and biofertilization were highly significant for the yield of examined plants. The yields were higher on inoculated treatments both in conventional (5–26%) and organic (7–15%) growing system.
The field experiment on Stevia rebaudiana (SR), (Family: Asteraceae) was carried out in acid soil zone of Shimoga (Karnataka) in the year 2009 and 2010 to investigate the influence of biofertilizers on protein, moisture and ash content in the dried Stevia leaf and their correlation with swelling property, water absorption capacity and mineral element contents. Results revealed that second harvested sample (August) in first year (2009) was better for all aspects than other harvested samples and the results showed significant increase in protein content (16.22 %), swelling index (SI) (5.10 % w/w), water absorption capacity (WAC) (4.91 ml/g) with the treatment T8 where three bio fertilizers were applied togetherly with simultaneous moisture content varied from 6.61 to 7.18 %. The significant higher correlations were observed (significant at 1 %) between protein content with SI (r = 0.99), WAC (r = 0.99) and SI with WAC (r = 0.98) in 2009 harvested samples. The ash content was higher in third harvested sample (13.54 %) in the year 2009 with simultaneous increased amount of Fe (6.19 mg/kg), Mn (1.24 mg/kg), Cu (0.72 mg/kg) and Zn (1.22 mg/kg) contents due to the residual effect of bio fertilizers. Nitrogen (0.74 mg/kg), P (0.40 mg/kg) and K (0.68 mg/kg) contents were higher during second harvested samples and thereafter decreased. Total phenolic content was also recorded highest (64.52 mg/g) with three bio fertilizers applied togetherly during year 2009 compared to the year 2010 (50.12 mg/g). The results suggest that the application of bio-fertilizers either single or more numbers in a balanced way may be beneficial in improving protein, phenol and mineral contents of stevia plant.
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