Wyniki wyszukiwania

1

Effect of amphotericin B on the biochemical markers in the haemolymph of honey bees

100%

Bajda M., Los A., Merska M.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 12

We examined the effect of amphotericin B (AmB) on the following enzymatic markers: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and on non-enzymatic markers: glucose, triglycerides, and proteins in the haemolymph of a model organism, Apis mellifera. AmB is an antifungal antibiotic. Despite its toxicity, it is used to treat disease conditions. The haemolymph of honey bees is considered as an analogue of human blood, and changes in marker values indicate pathological states, both in humans and honey bees. Three groups of caged bees were fed sugar syrup (the control group). The syrup was supplemented with AmB at concentrations of 0.25 mg/ml (AmB-25) and 0.50 mg/ml (AmB-50). The authors observed that the biochemical markers were age-related in the control group. Decreased values of the enzymatic markers in the AmB-treated groups confirm that AmB has a negative effect on the organism. The higher the dose of the antibiotic, the greater the increase in the concentration of the non-enzymatic markers. Our research shows that honey bees are an important model for studying the effects of AmB.

2

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Rapid evaluation of germinability of primed China aster (Callistephus chinensis Ness.) seeds with physiological and biochemical markers

86%

Badek B., Romanowska-Duda Z., Grzesik M., van Duijn B.

Journal of Horticultural Research

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2014

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tom 22

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nr 2

3

Ethylene production as a biochemical marker for radish seed vigour

86%

Holubowicz R., Tylkowska K., Gruchala L.

Folia Horticulturae

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1993

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tom 05

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nr 1

4

Influence of bradykinin and ACE inhibitors on selected biochemical markers of oxidative stress in rats with induced ischemia-reperfusion syndrome

72%

Krauss H., Sosnowski P., Grzymislawski M., Kozlik J., Jablecka A., Balcer N., Bednarek A.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 09

1052-1056

Cessation of blood flow in tissues leads to depletion of energetic resources of cells, disturbances in the activity of enzymes of the oxidative chain and electrolyte disorders, as well as to the production of reactive oxygen species. A great deal of attention has been paid to those problems since they are responsible for most of the complications in the ischemia-reperfusion (I/R) syndrome. Understanding the antioxidative mechanisms involved in I/R syndrome provides us with the potential to correct at least some of the resulting disturbances. One of the potentially beneficial factors is insulin. This observation directed our attention to the insulin-like factors, kinins (especially bradykinin), the activation of which was detected in I/R syndrome. The effect of bradykinin is modified by concurrent administration of the specific antagonists of bradykinin receptors, B1 and B2. The aim of the current study was to assess the role of bradykinin. In the study, the employed agents included the angiotensin converting enzyme inhibitor, which prevents the degradation of endogenous kinins, and blockers of bradykinin receptors, which abolish the influence of kinins. We observed that after 4 hours of ischemia followed by reperfusion, levels of free radicals in tissues as well as levels of peroxides in plasma increased significantly. Administration of bradykinin, captopril or enalapril resulted in the decline of these free radical levels. The application of B2 receptor antagonist decreased the beneficial influence of bradykinin, whereas B1 receptor antagonist revealed no significant effect. Activities of antioxidative enzymes (superoxide dismutase, catalase, and glutathione peroxidase) were measured. After 4 hours of ischemia and consecutive steps of reperfusion, a statistically significant increase in their activities was observed when bradykinin or ACE inhibitors were administered. Application of B2 receptor blockers reduced the effect of bradykinin, whereas the effect of B1 receptor antagonists was minute.

5

Decreased serum adiponectin reflects low vitamin D, high interleukin 6, and poor physical performance in knee osteoarthritis

72%

Udomsinprasert W., Manoy P., Yuktanandana P., Tanavalee A., Anomasiri W., Honsawek S.

Archivum Immunologiae et Therapiae Experimentalis

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2020

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tom 68

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nr 3

6

Quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen obtained after hormonal stimulation with Ovaprim or Ovopel

72%

Cejko B. I., Kucharczyk D., Targonska K., Kubiak D., Sarosiek B., Glogowski J.

Archives of Polish Fisheries

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2008

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tom 16

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nr 2

The aim of the study was to determine the basic quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen after spermiation was stimulated with Ovaprim and Ovopel. Sperm motility and concentration, osmotic pressure, total protein content, and activities of acid phosphatase (AcP), lactate dehydrogenase (LDH), and b-N-acetylglucosaminidase (b-NAG) were determined. It was revealed that higher sperm motility and concentration and higher seminal plasma protein content were obtained after stimulation with Ovaprim. The osmotic pressure of the seminal plasma estimated for males following the administration of Ovopel was higher than after they had been treated with Ovaprim. It was determined that enzyme activity in the seminal plasma of the fish stimulated with Ovaprim was higher in comparison with results obtained after they had been treated with Ovopel. Significant, positive dependencies were confirmed between the concentration of sperm and the total protein content in the seminal plasma (r2 = 0.492) and the activity of b-NAG (r2 = 0.779); among the total protein content in the seminal plasma and the activities of AcP (r2 = 0.476), LDH (r2 = 0.564), and b-NAG (r2 = 0.738); and between the activities of AcP and LDH (r2 = 0.483) and between the activities of LDH and b-NAG (r2 = 0.844).

7

Variety discrimination in pea [Pisum sativum L.] by molecular, biochemical and morphological markers

72%

Smykal P., Horacek J., Dostalova R., Hybl M.

Journal of Applied Genetics

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2008

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tom 49

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nr 2

155-166

The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. We chose 25 varieties of pea (Pisum sativum L.) from the list of recommended varieties for cultivation in the Czech Republic, and made both a standard classification by 12 morphological descriptors and a classification by biochemical-molecular markers. Two isozyme systems, 10 microsatellite loci, 2 retrotransposons for multilocus inter-retrotransposon amplified polymorphism (IRAP), and 12 retrotransposon-based insertion polymorphism (RBIP) DNA markers were analysed. The main objective of the study was to examine the potential of each method for discrimination between pea varieties. The results demonstrate a high potential and resolving power of DNA-based methods. Superior in terms of high information content and discrimination power were SSR markers, owing to high allelic variation, which was the only biochemical-molecular method allowing clear identification of all varieties. Retrotransposon markers in RBIP format proved to be the most robust and easy to score method, while multilocus IRAP produced informative fingerprint already in a single analysis. Isozyme analysis offered a fast and less expensive alternative. The results showed that molecular identification could be used to assess distinctness and complement morphological assessment, especially in cases where the time frame plays an important role. Currently developed pea marker systems might serve also for germplasm management and genetic diversity studies.

8

Związek białek zapasowych z zimotrwałością u wybranych mieszańców pszenicy ozimej

72%

Witkowska K., Witkowski E., Waga J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2013

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nr 268

9

Effect of nanoparticles of copper and copper sulfate administered in ovo on hematological and biochemical blood markers of broiler chickens

72%

Mroczek-Sosnowska N., Batorska M., Lukasiewicz M., Wnuk A., Sawosz E., Jaworski S., Niemiec J.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2013

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tom 52

At the first stage of the study the experimental material included 300 clutching eggs of Hubbard Flex chickens. The eggs were divided into three groups: control (without injection in ovo), Nano50 (in ovo injection of colloidal copper nanoparticles) and NanoCuSO4 (in ovo injection of colloidal nanoparticles of copper sulphate). Experimental solutions were administered by in ovo injection using a sterile needle 0.3 mm as follows: Nano50 group - colloid of copper nanoparticles (concentration: 50 ppm), and NanoCuSO4 group - colloid of copper sulfate (concentration: 50 ppm), to the air cell of the egg. The eggs were incubated under standard conditions. After hatching, 50 chicks were selected from each group for 42-day rearing. Birds were fed standard complete feed mixtures for broilers. On the last day of rearing (day 42), 12 females and 12 males were selected from each group and their blood was sampled for assays of hematological and biochemical markers. Hematological analyses included determinations of: WBC, RBC, Hb, heterophils, lymphocytes, monocytes, eosinophils and basophils, whereas biochemical analyses included assays of the following markers in blood serum: glucose, cholesterol, triglycerides, HDL-cholesterol, urea, calcium, magnesium, phosphorus, iron, ASPAT, and ALAT. The use of copper nanoparticles evoked an increase in blood levels of RBC, HGB, HTC, heterophils, monocytes and basophils. In addition, in blood serum in contributed to reduced concentrations of glucose and cholesterol and increased levels of selected microelements: calcium, phosphorus and iron.

10

Biochemical markers of tropospheric ozone: experimentation with test-plants

72%

Borowiak K., Rucinska-Sobkowiak R., Rymer K., Gwozdz E. A., Zbierska J.

Polish Journal of Ecology

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2009

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tom 57

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nr 1

3-14

The present study investigated the potential role of selected antioxidant enzymes: superoxide dismutase (SOD) and peroxidase (POX) as biochemical markers of the impact of tropospheric ozone. Experiments were carried out in ambient air conditions with two tobacco cultivars: Bel W3 and Bel B, which are sensitive and resistant to ozone, respectively. In this study, the degree of leaf injury of the sensitive cultivar was used as an indicator of the ozone level in correlation to the enzyme activity of both tobacco cultivars. In spite of low levels of tropospheric ozone during experimental season, the increase of antioxidant enzyme (SOD and POX) activity concomitant with the increase of ozone concentration was noticed in the sensitive cultivar as well as in the resistant one. This observation is especially important for the resistant tobacco, which does not exhibit any visual effects of ozone influence. Our results could be extrapolated to other plant species (i.e. Poaceae, Fabaceae, Solanaceae, Betulaceae, Salicaceae, Pinaceae), which do not reveal visible lesions in response to ozone stress.

11

Variability of selected hematological and biochemical markers in marathon runners

72%

Kaminska J., Podgorski T., Pawlak M.

Trends in Sport Sciences

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2015

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tom 22

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nr 3

Introduction. The marathon is an increasingly popular form of athletic competition, especially among amateur runners. It seems, therefore, important to determine how this type of exercise affects the runner’s body. Aim of Study. The aim of the study was to identify hematological and biochemical changes in the blood of amateur runners after a marathon run. Material and Methods. The study involved 10 male runners aged from 28 to 42 years. Their blood was collected from the fingertip four times: a day before the competition and one, three and five days after the race, and subjected to hematological and biochemical analysis. Results. The results revealed a statistically significant increase in the number of leukocytes, including monocytes and granulocytes, creatine kinase (CK) and lactate dehydrogenase (LDH) activity, urea concentration, and ferric reducing ability of plasma (FRAP). On the other hand, the values of certain indices, such as the number of erythrocytes, hemoglobin concentration, hematocrit, and red blood cell distribution width, decreased. There were no statistically significant changes in the number of lymphocytes and thrombocytes, the concentration of total protein, albumin, and polyphenols. Conclusions. Prolonged and intense physical eexercise, such as a marathon run, causes multiple significant changes in the body. These changes can lead to health disorders and deterioration of physical fitness.

12

Cytochrome b560 in the eggs of whitefishes as a biochemical marker on the family Coregonidae

72%

Tcherniaev G. A., Valyushok D. S., Artzatbanov V. Y., Mikulin A. E.

Polskie Archiwum Hydrobiologii

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1992

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tom 39

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nr 3-4

13

Genetic determination of cervid antlers in relation to their significance in social interactions

72%

Hartl G. B., Apollonio M., Mattioli L.

Acta Theriologica. Supplement

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1995

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nr 03

Antler traits (length of the main beam, circumference of the main beam, coronet circumference, and the number of antler points) in roe deer Capreolus capreolus (Linnaeus, 1758) from a population in Casentino (Northern Tuscany) were examined for associations with genotypes at loci coding for enzymes. Significant associations were found only in yearlings. Individuals homozygous for the allele Mpi120 had significantly larger antlers than carriers of other genotypes at Mpi. Individuals homozygous for Pep-2100 had significantly smaller antlers than carriers of other genotypes at Pep-2. In adults the results were essentially the same, but only as a statistically insignificant trend. The data were interpreted in terms of one or more genetic components having a major influence on antler development only in yearlings. This corresponds with behavioural data suggesting that in adults antler size is not related to reproductive success, but in yearlings it is correlated with sexual maturity and the rate of being expelled from the home range of their mothers by territory holders. The situation in the roe deer was compared with previous results on the genetics of antler development in the red deer, where males are social rather than solitary, yearlings do not participate in reproduction, and some antler characteristics are related to reproductive success of adult stags.

14

Biochemical markers of coregonids in Czechoslovakia

72%

Slechtova V., Slechta V., Valenta M.

Polskie Archiwum Hydrobiologii

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1992

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tom 39

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nr 3-4

15

Age- and BMD-related differences in biochemical markers of bone metabolism in rural and urban women from Lublin Region, Poland

72%

Filip R. S., Zagorski J.

Annals of Agricultural and Environmental Medicine

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2004

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tom 11

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nr 2

The utility of biochemical markers of bone metabolism has not been proven in the diagnosis of metabolic diseases of the bone tissue; however they are widely used as a tools for treatment monitoring. Their serum concentrations are influenced by a number of factors, like gender, health status, anthropometric and environmental factors. All the factors listed above should be taken into consideration during clinical use. The aim of the study was to determine the reference values and evaluate the influence of environmental and anthropometric variables on biochemical markers of bone turnover for women from Lublin Region (Poland). Subjects of the study were 188 normal women aged 30-79, all residents of Lublin Region. Analysed markers of bone turnover were: osteocalcin (OC) and C-terminal cross-linking telopeptide of type I collagen (CTX-I), both assessed using ELISA method. All blood samples were taken and analyzed at the Clinical Chem. Laboratory and Patho-morphology Department at the Institute of Agricultural Medicine in Lublin. The lumbar spine (L2-L4) of all subjects was examined in a-p position using the dual X-ray absorptiometry-DXA (DPX-A LUNAR Corp.) at the Department of Metabolic and Degenerative Diseases of Bone Tissue of Institute of Agricultural Medicine in Lublin. Data pertaining to factors affecting bone tissue were collected using a specially prepared questionnaire. Serum levels of OC and CTX-I in women in every age range were different, generally increasing with age. Serum levels of OC and CTX-I in the analysed population strongly depended of both menopausal status and bone mineral density. In conclusion, this study demonstrates that age and menopausal status variations need to be considered when interpreting laboratory measurements of biochemical markers of bone metabolism.

16

Biochemical markers of psoriasis as a metabolic disease

58%

Gerkowicz A., Pietrzak A., Szepietowski J. C., Radej S., Chodorowska G.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 2

17

Impact of products from ground buckwheat added to balanced diets on biochemical blood markers in Wistar rats

58%

Orzel D., Zmijewski M., Bronkowska M.

Roczniki Państwowego Zakładu Higieny

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2015

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tom 66

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nr 3

Background. It is believed that buckwheat can be used as a functional food ingredient in the prevention and treatment of diet-related diseases, e.g., atherosclerosis, hypertension, obesity, constipation and cancers. The use of buckwheat protein preparations in the diet for experimental animals had a significant effect on the reduction of cholesterol level in their blood serum, liver and gall bladder, and additionally inhibited the formation of gall stones as a result of changes in cholesterol metabolism. Buckwheat protein extracts have additionally been shown to reduce the level of LDL and VLDL fractions. Objective. The aim of this study was to evaluate the effects of different products from ground buckwheat nuts (flour, meal and bran) and bread with their addition applied in balanced diets on selected biochemical blood markers in Wistar rats. Material and Methods. The study was conducted with 64 male Wistar rats. Animals received a semi-synthetic diet AIN 93M with 20% addition of buckwheat flour, meal or bran and containing 20% rye-buckwheat bread made with 35% buckwheat flour, meal or bran, respectively. The animals were fed the experimental diets for 4 weeks. Whole blood was used to measure hematocrit and hemoglobin concentrations. The contents of glucose, total cholesterol, LDL, HDL, and triglycerides were determined in the blood serum using BioSystem biochemical tests. Results. There was no significant effect of 20% addition of ground buckwheat products (buckwheat flour, meal and bran) or bread with their addition to balanced diets on hematocrit, hemoglobin concentration in the blood or the concentration of glucose in the blood serum of experimental animals. The study showed a statistically significant beneficial effect of dietary addition of buckwheat or rye-buckwheat bread on the reduction of total cholesterol, LDL cholesterol, and triglycerides (TGC) in the blood serum of rats. Conclusions. A statistically significant decrease was demonstrated in LDL cholesterol and triglycerides in the blood serum of animals fed diets with 20% addition of ground buckwheat products (buckwheat flour, meal and bran) and rye-buckwheat bread containing these products.

18

Identyfikacja odmian ziemniaka metoda elektroforetyczna

58%

Przewodowski W., Lewosz J., Treder K., Barnyk A., Pilecki T.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2007

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nr 243

151-157

19

Hawthorn revisited: time- and dose-dependent cardioprotective action of WS-1442 special extract in the reperfusion-induced arrhythmia model in rats in vivo

58%

Zorniak M., Porc M. P., Krzeminski T. F.

Journal of Physiology and Pharmacology

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2019

|

tom 70

|

nr 2

20

Comparison of cellular and biochemical markers of airway inflammation in patients with mild-to-moderate asthma and chronic obstructive pulmonary disease: an induced sputum and bronchoalveolar lavage fluid study

58%

Gorska K., Krenke R., Domagala-Kulawik J., Korczynski P., Nejman-Gryz P., Kosciuch J., Hildebrand K., Chazan R.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 6

271-283

Ograniczanie wyników

Effect of amphotericin B on the biochemical markers in the haemolymph of honey bees

Rapid evaluation of germinability of primed China aster (Callistephus chinensis Ness.) seeds with physiological and biochemical markers

Ethylene production as a biochemical marker for radish seed vigour

Influence of bradykinin and ACE inhibitors on selected biochemical markers of oxidative stress in rats with induced ischemia-reperfusion syndrome

Decreased serum adiponectin reflects low vitamin D, high interleukin 6, and poor physical performance in knee osteoarthritis

Quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen obtained after hormonal stimulation with Ovaprim or Ovopel