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Introduction: Solanum sodomaeum L. has been observed to have several medicinal properties, in particular, in the treatment of several types of human skin cancer. Objective: The influence of the maturation stage of S. sodomaeum fruits on the total lipid contents, fatty acid profiles, essential oil yields and compositions, as well as the antibacterial and antioxidant activities of the essential oils, was investigated. Methods: The fatty acid and essential oil constituents were identified using gas chromatography (GC) and GC–mass spectrometry (GC–MS). The antioxidant properties of essential oil and vegetal oil were assessed using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging and reducing power assays. The antibacterial ac-tivity of essential oil was tested using the disc diffusion assay for resistance in human pathogenic bacteria. Results: Mature fruits showed higher total lipid content (17%) and were characterised by polyunsaturated fatty acids (53.87%), represented mainly by linoleic acid (53.11%). Similar yields of essential oils were detected for immature (0.43%) and mature (0.45%) fruits. Tetrahydronaphthalene (41.79%) was detected as the major essential oil component at the immature stage versus dihydrocoumarin pentane (18.27%), hexadecanoic acid (17.43%) and 2-undecanone (13.20%) in mature fruits. The DPPH test showed that essential oils had better antioxidant properties; however, the vegetal oils showed better performance in the reducing power assay. Moreover, the essential oil of S. sodomaeum mature fruits was active against bacterial strains. Conclusions: S. sodomaeum fruits could be a valuable source of natural antioxidants and antibacterial agents.
ntroduction: Powder extracts from white mulberry leaves (Morus alba L.) are desirable due to their simplicity of use. Powders intended for use in food and dietary supplements can be obtained by various methods, including water extraction and spray drying. Objective: The aim of the work was to optimize the aqueous process of extraction of white mulberry leaves and characterization of bioactive properties of the obtained extract powder. Methods: The DNJ and flavonoids (quercetin, kaempferol) contents and were analysed by high performance liquid chromatography (HPLC). Total phenolic contents were determined using the Folin-Ciocalteu phenol reagent and antioxidant activity by ABTS and DPPH assays. Results: The mulberry leaf extraction process has been optimized. The obtained powdered mulberry leaf extract proved to be a good source of bioactive compounds. Characteristic phenolic compounds detected in mulberry leaves and their powder extract were quercetin and kaempferol. The sum of polyphenols was 10.9 mg GAE/g dry matter in the raw material, whereas in the powder extract 42.6 mg GAE/g dry matter. In addition, it was found that the obtained powdered extract is characterized by a five-fold higher, in comparison to the raw material, the content of antioxidant activity measured by ABTS and DPPH tests. The content of 1-deoxynojirimycin (DNJ) in the powder extract was nearly four times higher than in dry mulberry leaves. Conclusion: The optimized process of water extraction of white mulberry leaves allows to keep valuable bioactive components and to obtain their high concentration.
This study aimed determining the contents of soluble protein, free amino acid, phenolic, flavonoid, soluble carbohydrate, sugars (glucose, fructose and sucrose) and elements in selected wild growing and cultivated mushroom species collected from various locations of Turkey. Significant differences (P < 0.05) were found for the contents of total free amino acid, soluble protein, phenolic, flavonoid, soluble carbohydrate and sugars. The total free amino acid, soluble protein, phenolic, flavonoid and soluble carbohydrate contents of mushrooms ranged from 33.57–126.57 mg g–1, 2.77–7.55 mg g–1, 28.68–157.39 mg g–1, 8.55– 30.66 mg g–1 and 59.89–343.55 mg g–1, respectively. Elemental analysis showed that mushrooms contained significant amounts of potassium (1345.07–9310.17 mg kg–1), phosphorus (1462.44–6159.45 mg kg–1), calcium (18.78–349.15 mg kg–1), sulphur (952.41–12486.63 mg kg–1), iron (80.62–606.26 mg kg–1), manganese (22.65–147.57 mg kg–1), zinc (103.26–522.81 mg kg–1) and selenium (0–115.40 mg kg–1). Nutritient composition varied with mushroom species. The means of total soluble protein, total phenolic, total flavonoid, potassium, phosphorus, sulphur, chlorine, sodium, iron, calcium, manganese, selenium, zinc and copper contents in wild growing mushrooms were found higher than cultivated mushrooms.
Cyanobacterial biofilms serve as food and shelter for benthic invertebrates, such as juvenile insects. Chironomids are often the most widely distributed and abundant insect larvae in freshwater ecosystems. As a consequence of high grazing pressure, effective defence mechanisms can be expected in biofilm-forming organisms. The presence of chemical defence was studied in 12 axenic and monoxenic cyanobacterial species. Flakes of cyanobacterial biofilms were offered to Chironomus riparius (Meigen) over a period of 8 days. Mortality and body-length of the surviving animals were used as indicators for the toxicity of the cyanobacteria and their suitability as food. Toxicity and inhibition of larval growth were found for several cyanobacteria tested. Fischerella sp. (ATCC 43239) was the most active and caused 100% mortality in Chironomus larvae within 24 h. Mortality was also high (87%) for larvae fed with Aphanothece sp. Moderate toxicity (40–60% mortality) was found for Calothrix sp. (PCC 7507), C. braunii Bornet et Flahault, C. thermalis (Schwabe) Hansgirg and a cyanobacterium of the LPP group designated JU 5. Mortality of 7–33% was observed for Calothrix parietina (Nägeli) Thuret, Oscillatoria brevis (Kützing) Gomont, Cylindrospermum sp., Nostoc sp., Calothrix anomala Mitra and a cyanobacterium of the LPP group designated 5 KB. Differences depending on the cyanobacterial food offered were also seen in the lengths of surviving larvae. Fischerella sp. (ATCC 43239) was studied in more detail to chemically characterise the observed insecticidal activity. The insecticidal activity could be extracted with 60% aq. methanol from the fresh biomass and caused 100% mortality in Chironomus. A literature survey was performed on the bioactive compounds so far isolated and characterised from Fischerella and related Stigonematales. It is noticeable that no insecticidal activity has been shown for any of these compounds yet. The newly found insecticidal property of Fischerella may lead to the identification of bioactive compounds which may be important as chemical defence against insect grazers.
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