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The research objective was to determine the degree of microbial contamination of the soil from a silver fox farm and animal feces in accordance to the season of the year and sampling location. The air temperature and relative humidity as well as sample moisture at the sampling sites were also evaluated. The studies were performed from October until September. Soil samples were collected from under the cages (GI), between the rows of the cages (GII) and at a distance of 30 m from the cages (GIII), whereas fox feces were taken from under the cages (KI), between the rows of the cages (KII) and 45 m from the cages (KIII). The soil and feces samples underwent qualitative and qualitative microbial assessment. The total count of mesophilic, psychrophilic, proteolytic bacteria, actinomycetes, from the group of coli and E.coli was established, according to the Polish Norms. The qualitative evaluation included genus identification of bacteria from the family Enterobacteriaceae in compliance with commonly applied procedures. The highest bacterial count under study was found in October in the soil samples from under the cages (GI). Bacteria E. coli and Salmonella rods were recovered from the soil (GI) and (GII) throughout the year, while Enterobacter spp. and Citrobacter spp. were isolated only from some GI samples. The highest average number of bacteria in fox feces was also established in the samples collected from under the cages at the turn of December and January. It was found that increasing relative humidity significantly decreased the count of all the bacteria studied in fox feces, whereas elevated air temperature contributed to declining numbers of psychrophilic bacteria and from the coli group. In the feces samples taken throughout the research period E. coli, Salmonella spp. and Shigella spp. bacteria occurred, while Klebsiella spp., Enterobacter spp. were isolated in single samples. The growth of all the studied bacteria was affected by relative humidity and sample moisture, whereas psychrophilic bacteria and from the coli group by air temperature. Microbial contamination of the environment is substantially influenced by the season of the year and the pertaining atmospheric conditions, as the largest bacterial load in soil and feces was determined in autumn and winter. The highest bacterial numbers occur in soil and feces collected from under the cages, which is associated with increased organic matter (feces and feed leftover) content and medium moisture optimal for bacteria. Therefore, it is recommended to undertake preventive measures within the sanitary-veterinary supervision aiming at improvement of the state of health of fur bearing animals.
The purpose of the research was to evaluate the chilling environment - the waters from the spin-chillers and the air from the chilling rooms - on the bacterial contamination of broiler chicken carcasses after slaughter. The research was conducted on two chilling systems for poultry used in Polish slaughterhouses: immersion and evaporative chilling. Forty samples of water from the spin-chillers underwent microbiological analysis as well as 20 samples of air from chilling rooms of both chilling systems. The following were determined from the above-mentioned materials: the total count of aerobic bacteria and coliforms, as well as the psychrotrophic and proteolytic groups. The presence of Salmonella was only evaluated in the water samples from the refrigerators (20 samples from each of the systems). A significant influence of the type of chilling system on the contamination of the water from the spin-chillers has been demonstrated on all the evaluated groups of bacteria. Water derived from the evaporative chilling system contained significantly more microflora (8.9 × 10³ cfu/ml) in comparison to the immersion system (7.0 × 10³ cfu/ml), which might have been caused by the manual eviscerating of the carcasses. The chilling system varied the percentage of particular groups of evaluated bacteria in the total microflora contamination in the water from the spin-chillers. Depending on the chilling system the percentage of the coliforms was 5-9% of the total count of bacteria, the percentage of psychrotrophic bacteria from 43-52%, proteolytic bacteria from 27-40%. The presence of Salmonella was confirmed in the water from the spin- -chillers of both of the systems. They were isolated more frequently in the immersion system (90% of the samples were positive) than in the evaporative chilling (50%). The most frequently isolated serotype was S. Enteritidis, the presence of which was confirmed in half of all water samples under examination. The remaining serotypes (S. Agona, S. Infantis, S. Hadar and S. Cremieu) were isolated less frequently. The analysis of the microbiological contamination of the air from the chilling rooms only demonstrated significant differences between both systems in the Coli and proteolytic groups. The count of the above-mentioned bacteria in l m³ of air was lower in a chilling room of the immersion system than in an evaporative chilling. There were no differences in the total count of bacteria and in the psychrotrophic bacteria. The total contamination in l m3 of air in the immersion system amounted to 2.9 × 10³ cfu. The count of coliforms in this system was 6.8 × 10 cfu/m³, which constituted 2.34% of the total count of bacteria, while in the evaporative chilling it was 2.7 × 10² cfu (10%). The psychrotrophic bacteria contamination constituted l.4 × 10³ (48.27%) in the immersion and l.6 × 10³ (59.25%) in the evaporative chilling. Proteolytic bacteria constituted from 10% in the immersion to 33% in the evaporative chilling of the total count of bacteria. The chilling room environment has a significant impact on the bacterial contamination of broiler chicken carcasses in the final phase of their production - primarily through the water from the spin-chillers, but also as a result the air movement.
The aim of the study was to establish the variation in microbial contamination of quail eggshell surfaces depending on the frequency of hygiene-sanitary practices carried out in cages (washing, disinfection and fecal waste removal). The studies involved eggs collected from three quail breeding farms situated in south-eastern Poland and characterized with different frequencies of hygiene practices. From October to April, 50 eggs were collected from each farm at five different collection times, at 3-week intervals. The freshly laid eggs were collected in a sterile way directly from the cages, cooled and transported to the laboratory to be subjected to a rinsing treatment with a sterile solution technique to obtain 10–1/cm2 dilution of the eggshell surface. The obtained study material was evaluated for a total count of aerobic bacteria, numbers of proteolytic bacteria and bacteria from the Staphylococcus genus, counts of aerobic bacteria and microorganisms from the Enterobacteriaceae family, as well as the number of yeasts and moulds. As per entire egg surface, the presence of pathogenic microorganisms from the Salmonella, Campylobacter and Listeria genus were detected. The determinations were performed in accordance to Polish Standards. Relatively slight contamination of eggshell surfaces was established (from 4.7 × 102 up to 4.7 × 103 cfu/cm2 ), and its contamination level varied between the farms. Out of the microbial groups under study, Staphylococcus spp. constituted the predominant part of microbial flora (32.7-51.5%), yet only a single sample was found to harbor coagulase-positive staphylococci (Staphylococcus aureus subsp. anaerobius). The presence of neither moulds nor yeasts was confirmed on the shell of eggs collected from farm 1, while their counts on the eggs obtained from two other farms did not differ significantly and ranged between 0.8 × 102 and 1.2 × 102 . In two samples, microorganisms from the Listeria genus were recovered; however, the PCR studies did not confirm their belonging to the L. monocytogenes species. The presence of Campylobacter and Salmonella genus bacteria on the eggshells under study was not detected. It was found that frequency of hygiene-sanitary practices carried out at a farm had a significant effect on the level of microbial contamination of quail eggshell surfaces. The cleaning of cages (washing and disinfection) together with daily removal of feces considerably reduced microbial contamination of eggshells. The absence of bacterial pathogens of Campylobacter and Salmonella genus and L. monocytogenes on the studied eggshell surfaces and a low level of total microbial contamination indicate that quail eggs are safe for consumers on condition that sanitary regulations are observed.
The aim of the study was to determine the influence of the maintenance system of laying hens on the bacterial pollution of the egg shell surfaces of consumer eggs. Eggs derived from four acceptable maintenance systems of hens were selected for the investigations: caged, litter, free ranging, ecological, whose source was from the Lublin voivodeship and obtained during the spring and summer period (April – July). 200 eggs were examined, 50 from each of the examined maintenance systems. All the examined eggs belonged to the L weight class. The egg surface was calculated using Pagannelli et. al.’s formula: Ps = 4.835 × M⁰·⁶⁶². Next the egg was rinsed with a sterile liquid, attaining a 10⁻¹/cm² thinner egg shell surface, which was again thinned down by a tenth. In the examined material the following were determined: the general oxygen bacteria count, the number of proteolitic and psychrophilic bacteria, bacteria from the Enterobacteriaceae family, as well as micro-organisms of the Enterococcus and Staphylococcus type. The measurements were conducted according to Polish norms. The bacteria count (the units forming a colony) was presented in a decimal logarithmic form, and subsequently expressed as log jtk/cm² of an egg shell. It has been confirmed that the total number of oxygen bacteria on the eggs was: from caged hens log 4.95 jtk/cm²; litter, log 5.35 jtk/cm²; free ranging, log 5.25 jtk/cm²; while from ecologically sustained hens, log 5.18 jtk/cm². The pollution on eggs from the caged maintenance system was significantly lower than those from the litter system. The proteolitic bacteria count on eggs from the maintenance systems was log 4.19 from the caged, log 4.71 from litter, log 4.72 from free ranging, and log 4.54 jtk/cm² from the ecological. Significant differences in pollution were confirmed in this type of bacteria between the caged and litter as well as free ranging maintenance systems. The psychrophilic bacteria pollution ranged from log 3.66 jtk/cm² of an egg shell in the caged system to log 4.02 jtk/cm² in the litter system. Significant differences in pollution in this type of bacteria occurred only between the caged and litter systems. The bacteria count of the Enterobacteriaceae family ranged from log 0.65 jtk/cm² on eggs derived from the litter system to log 1.64 jtk/cm² from hens kept in the free ranging maintenance system. Significant differences in pollution were determined in this type of bacteria between the litter, free ranging and both of the remaining examined maintenance systems. The bacteria count of the Enterococcus type in the case of the caged system was log 1.08, litter log 3.10, free ranging log 2.34 and log 2.08 jtk/cm² from the ecological maintenance system. Significant differences in bacteria of the Enterococcus type were determined between the caged and litter systems, and both of the remaining examined maintenance systems, which did not differ among themselves. In the case of the bacteria of the Staphylococcus type, the lowest pollution was determined on the egg shells derived from the caged system – log 2.73 jtk/cm², while the greatest in the free ranging (log 4.36) and litter (log 4.58 jtk/cm²) systems. It was confirmed that the maintenance system of laying hens has a significant influence on bacterial pollution of the consumer egg shells. The highest hygienic quality was demonstrated by eggs from the caged system, the lowest from the litter and free ranging systems.
Badania przeprowadzono w doświadczeniu modelowym na glebie płowej wytworzonej z piasku gliniastego mocnego. Celem pracy było zbadanie wpływu dawki 500 kg S ha-1 r-1 na liczebność bakterii i grzybów proteolitycznych, aktywność respiracyjną i dehydrogenazową w glebie płowej wzbogaconej gnojowicą (60 m3 ha-1 r-1). Przeprowadzone badania wykazały, że zastosowane zasiarczenie z równoczesnym wzbogaceniem gleby gnojowicą wpływało stymulująco na liczebność bakterii proteolitycznych. Na liczebność grzybów proteolitycznych istotny hamujący wpływ miała tylko dawka kwaśnego opadu Aktywność respiracyjna gleby płowej nie wykazywała różnic między badanymi obiektami doświadczalnymi. Aktywność dehydrogenazowa gleby zasiarczonej i wzbogaconej gnojowicą była najwyższa i istotnie różniąca się od pozostałych obiektów doświadczalnych.
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