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Probiotics and prebiotics play an important role in human and animal nutrition. Those research studies were performed to evaluate the potential of using legume sprouts as carriers for probiotic strain of Lactobacillus rhamnosus GG. They determined the effect of legume species, temperature of sprouting, and inoculation methods of seeds or growing sprouts on the survival and/or growth of probiotics. It was found that the count of bacteria in sprouts depended on the germination temperature, inoculation methods as well as on the species of legume used as a carrier. The beans examined (Adzuki and Mung) germinated effectively at a temperature between 25 ÷ 35 ºC. And the lentil sprouted most effectively at 25 ºC. In the case of soy-bean and lentil, the temperature of 35 ºC caused the germination efficiency to decrease. The growth of Lb. rhamnosus GG was reported only in the case of the lentil and soy-bean sprouts obtained from the seeds imbibed in an inoculum and germinated at 25 ºC. The count of probiotic bacteria was 3.1×10⁶ and 7.18×10⁶ CFU per grams of fresh mass, respectively. The sprouts obtained from the bean seeds analyzed did not provide any conditions for probiotic bacteria to survive and grow. The best carrier for the probiotic bacteria studied were the soy-bean sprouts; in their case, after inoculation of seeds and using a suspension of probiotic bacteria, the sprouts obtained at 25 ºC had the best quality parameters.
The purpose of the study was to elaborate a molecular procedure to identify the DUMPS mutation which causes hereditary disorder (deficiency of uridine monophosphate synthase) in cattle. The degree of this disorder was determined in a gene pool of a Polish bredcattle population which consisted of 1038 animals: 781 artifical insemination bulls from all centers in Poland and 257 sire dams from the two largest breeding centers of Holstein-Friesian cattle: Osięciny and Osowa Sień. The diagnosis was performed using the PCR-RFLP method and technique based on automated solid-phase sequencing of PCR products. In the examined material no DUMPS carrier was found. This indicates that the population of cattle reared in Poland is free from this economically significant disorder. The results obtained indicate a high effectiveness of monitoring of point mutation DUMPS by the PCR-RFLP method. Moreover, it is worth noting the wide possibilities of examining other biologically significant genome regions using the solid-phase sequencing method.
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