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The studies were carried out on 2-month-old breeding carp. The CERBIO probiotic was given with corn feed at a dose of 150 or 300 g/100 kg of fodder for 53 consecutive days. The usefulness of that feeding system was assessed on the basis of body weight increase of the fish and also by taking into account immunological indices (phagocytosis, metabolic activity of neutrophiles, activity of myeloperoxidase) and haematological ones (number of leucocytes and neutrophiles, blood picture). The administration of the probiotic proved to be profitable as the body weights of the fish under study were higher compared with control group, especially when the probiotic was given at a dose of 300 g/100 kg of fodder. Immunological - and to some extent haematological results showed that the parameters examined were higher in the experimental group than those in a control one.
The studies were done on 90 carps Ki of a 60-80 g weight exposed to permethrin at a concentration of 0.3 pg/L of water for 28 days (group 1) or 11 pg/L for 14 days (group 2). Immunological examinations were done with the blood of fish in group 1 after 4, 7, 14, 21, and 28 days, and in group 2 after 4, 7 and 14 days after the use of permethrin. Immunological examinations were performed after 7 and 14 days from the end of permethrin action. Group 3, untreated, served as a control. It was found that permethrin at a concentration of 0.3 pg/L significantly decreased metabolic activity of neu- trocytes and the level of blood lysozyme after 28 days of exposure. Permethrin at a concentration of 11 pg/L caused death of about 50% of the examined individuals after 14 days exposure. Granulocytosis, monocytosis and unphy- siological nuclear division in neutrocytes and lymphocytes were noted. During the whole period a significantly decreased metabolic activity of neutrocytes and blood plasma lysozyme level were observed. After transport of the fish into clean water, the examined parameters returned to physiological value in group 1 after 14 days, while in group 2 all the parameters had lower values than those in the control group.
Sixty four patients who had passed the acute phase of trichinellosis 3, 4, 5 and 8 months and 1, 2 and 4 years earlier, have been examined with respect to their clinical condition, persistence of possible subjective complaints which could be associated with the acute phase passed, leucocytosis, absolute number of acidophilic granulocytes in blood, indices of immunological response, both humoral (IgG, IgA and IgM) and cellular (percentage of T and B lymphocytes in blood), serotonine level in blood, monoaminooxidase activity in blood serum and passive hemaglutination reaction with trichinellosis antigen. It has been found that the frequency of occurrence and intensity of subjective complaints decreases with time. Changes in humoral and cellular reactivity, and long-lasting persistence of antibodies against the trichinellosis antigen, determined with passive hemaglutination method, have been also observed in particular patients.
The research objective was to assess the health of sea trout from the Pomeranian rivers Słupia, Wieprza, Parsęta, and Rega in 2010. Microbiological, hematological, and immunological examinations were performed. In total, twelve gram-negative bacteria species were isolated and identified; they belonged to the families Pseudomonadaceae, Enterobacteriaceae, and others. The gram-positive bacteria isolated belonged mainly to the genus Streptococcus. Immunological and hematological examinations did not reveal statistically significant differences among samples. In comparison to previous examinations, the quantity of infected fish was significantly lower.
Celem badań była próba prześledzenia przebiegu yersiniozy u świń od momentu zakażenia do zakończenia siewstwa. Badania przeprowadzono na 15 ośmiotygodniowych prosiętach. Zwierzęta podzielono na trzy równe grupy i zakażono: grupę A - dożylnie, grupę В - dożołądkowo, a grupa С stanowiła grupę kontrolną - niezakażoną. Do zakażenia użyto chorobotwórczego szczepu Yersinia enterocolitica 0:3 biotyp 4. W ciągu 14 tygodni trwania doświadczenia zwierzę­ta poddawane były obserwacji klinicznej i badaniom laboratoryjnym. Przeprowadzono m.in. badania bakteriologiczne kału, immunologiczne a po dokonaniu uboju diagnostycznego - badania bakteriologiczne wycinków narządów wewnętrznych oraz histopatologiczne i ultrastrukturalne. Na podstawie przeprowadzonych badań wykazano, że doświadczalne zakażenie świń drogą pokarmową wywołuje silniejszą reakcję kliniczną i odpowiedź immunologiczną niż zakażenie drogą dożylną. Pałeczka Yersinia enterocolitica jest silnym antygenem pobudzającym zarówno odporność komórkową, jak i humoralną, a także nieswoiste mechanizmy komórkowe. Nie stwierdzono zależności między poziomem przeciwciał a izolowaniem drobnoustrojów z kału.
The significance of eel to fisheries management and the environment necessitates the continuous monitoring of the health and condition of this valuable species. One of the many monitoring studies realized within the project was to determine the condition and health status of eel used for stocking open waters and those inhabiting various basins in Poland. The newest research and diagnostic methods were used to conduct these targeted studies. The studies were performed by a research team comprised of staff from the Department of Ichthyology at IFI, the Department of Fish Pathology and Immunology at IFI, the Department of Microbiology and Clinical Immunology and the Department of Epizootilogy in the Faculty of Veterinary Medicine at UW-M in Olsztyn, and the Department of Food and Environmental Chemistry at the National Marine Fisheries Research Institute in Gdynia. Full clinical, anatomic pathological, parasitological, mycological, virological, bacteriological, biochemical, and immunological studies were performed. The analysis of the condition and health status of stocking material indicated that eel originating from Polish and foreign hatcheries for stocking open waters did not resent and pathology that would indicate and acute or chronic diseases. Neither the EVEX or the AngHV-1 viruses were isolated from the fish analyzed, nor was the presence of the VHS, IHN, IPN, or SVC viruses confirmed. The analysis of the condition and health of eel inhabiting open waters indicated that approximately 20% of the fish from each basin exhibited abrasions, discoloration, small skin lesions, and bruising on the caudal and pelvic fins. Only in single specimens from some basins was pathology indicative of disease confirmed. The bacteria Aeromonas hydrophila, A. sobria and Flavobacterium psychrophilum were isolated from them. Targeted virological studies of one eel specimens from the Szczecin Lagoon confirmed the occurrence of the AngHV-1 virus. An important element of the research was that among the fish monitored from various basins a systematic increase in the prevalence of the nematode Anguillicoloides crassus was noted at an intensity and extensiveness of invasion that ranged from 50 to 100% of the studied fish population
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