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1

Identification and antibacterial resistance of bacteria isolated from poultry

100%
El-Rami F. E., Sleiman F. T., Abdelnoor A. M.
Polish Journal of Microbiology
|
2012
|
tom 61
|
nr 4
Food-borne infections are among the prominent health hazards. Antibacterial agents (ABA) are usually administered to poultry in Lebanon as antibiotic growth promoters (AGP), which might lead to the dissemination of resistant bacterial strains. e aims of this study were to isolate potential food borne pathogens from poultry and investigate an association between AGP usage and antibacterial resistance (ABR). Isolates were obtained from the culture of cloacae swabs and identified. Escherichia coli was the predominant isolate. There was a significant association between the use of tetracycline and gentamicin as AGP and the number of E. coli isolates resistant to these ABA.
2

Bacterial resistance and clinical effectiveness of combinations of beta-lactam with aminoglycoside antibiotic on the example of Vetramycin and Siccovet

100%
Kania B. F., Kania K., Sutiak V.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 07
3

Effect of soil pollution on bacterial resistance to lead ions: an experimental approach

86%
Blaszak M., Bienkowska D.
Polish Journal of Ecology
|
2009
|
tom 57
|
nr 3
555-560
The objective of the laboratory experiment was to study the resistance to lead ions of bacteria isolated from soil with various level of contamination with this element. Lead (II) acetate trihydrate was applied to the soil (sandy loam) in two doses so that the total content of lead corresponded to weak (250 mg kg⁻¹) and strong (5000 mg kg⁻¹) contamination of soil with lead. From each of the soil (control, weakly and strongly contaminated soil) bacterial isolates were obtained and several dozen of them were randomly chosen for the experiment. The isolates were severally point wise cultured in three repetitions onto microbiological medium contaminated with lead (1000 mg dm⁻³) and on the same control medium. The diameter of grown “twin” colonies in both cultures were compared. For each isolate separately the resistance index (RI) was described as a ratio of bacterial cultures diameter of the soil isolate cultured on the contaminated medium to the diameter of the same isolate cultured on the control medium. On the basis of the various RI results, the bacteria were divided into four resistance groups (RG) of approximate resistance index: I group, RI = 0 – entirely sensitive bacteria (no growth on the contaminated medium); II group 0
4

Odpornosc bakterii Clavibacter michiganensis subsp. michiganensis na dezynfektanty

72%
Kamasa J.
Progress in Plant Protection
|
2008
|
tom 48
|
nr 2
468-471
5

Analysis of Proteus mirabilis distribution in multi-species biofilms on urinary catheters and determination of bacteria resistance to antimicrobial agents

72%
Moryl M., Torzewska A., Jalmuzna P., Rozalski A.
Polish Journal of Microbiology
|
2013
|
tom 62
|
nr 4
The objectives of the investigation presented in this paper were: to examine the frequency of P. mirabilis isolation from catheters and assess the complexity of multi-species biofilms which these bacteria form, as well as to determine the vulnerability of planktonic and sessile P. mirabilis populations to popular antibiotics and compare it to the susceptibility of other Gram-negative bacteria isolated as associated flora from multi-species biofilm. 88 urological catheters, collected from long-term catheterized patients were examined. Uropathogens were recovered from the catheter surface by sonication, and identified on standard diagnostic media. The broth-microdilution method and the MBEC High-throughput Screening assay were used to determine the bacterial resistance to antibiotics. 279 microorganisms were isolated from 88 urinary catheter biofilms. The Enterobacteriaceae family were the most frequently detected bacteria (53.2% of isolates), whereas Proteus spp. isolation accounted for 17.9%, which placed these bacilli on the third position in the Enterobacteraceae family. Among all the tested drugs, amikacin and cephalosporins (ceftriaxone, cefotaxime and cefaclor) exhibited the highest activity against P. mirabilis planktonic cells, 86% and 73% of strains were susceptible to these antibiotics, respectively. 100% of P. mirabilis sessile forms were resistant to cefepime, ciprofloxacin, gatifloxacin, and norfloxacin. Amikacin and ceftriaxone affected only 5% of sessile forms. The planktonic cells of the other studied uropathogens were mostly vulnerable to the all tested drugs (exception P. aeruginosa strains), the most effective of which occurred to be amikacin and cefepime. Obtained MBECs values were 2-512-fold higher than MICs assessed for planktonic forms.
6

New methods of pathogenic bacteria elimination

72%
Wolska K. I., Kraczkiewicz-Dowjat A., Grudniak A. M., Sajkowska A., Wiktorowicz T.
Polish Journal of Microbiology
|
2004
|
tom 53
|
nr Suppl.
The growing bacterial resistance to antibiotics calls for the elaboration of new pathogens elimination strategies. Some of these methods are based on the conjugative transfer of recombinant plasmids able to eliminate pathogenic recipients by plasmid run-away replication or by killing activity of plasmid-encoded bacteriocins. Using live bacteria as donors of plasmid vectors carrying killing determinants requires meeting many safety restrictions in order to eliminate potential biohazard.
7

Survey of antimicrobial susceptibility among some pathogenic and commensal bacteria isolated from pigs in Lithuania

58%
Ruzauskas M., Klimiene I., Zienius D.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 04
397-400
Antimicrobial susceptibility of isolated enterotoxigenic E. coli, Salmonella enterica serovar Choleraesuis, Pasteurella multocida and Streptococcus suis from pigs was tested in the Veterinary Institute of the Lithuanian Veterinary Academy. Commensal E. coli and Enterococcus faecalis were also included in the testing as commensal bacteria. Clinical and pathological material was investigated from various regions of the country. Isolation and identification of bacteria was done using common methods. The agar diffusion method according to NCCLS guidelines was applied for antimicrobial susceptibility testing. Enterotoxigenic E. coli showed the highest resistance to tetracycline (67%), ampicillin (52%) and sulfamethoxazole-trimethoprim (43%). Not less than 90% of these bacteria were susceptible to colistin, florfenicol and ceftiofur. Salmonella Choleraesuis demonstrated the highest resistance to tetracycline (53%). Florfenicol, ceftiofur and enrofloxacin were effective against most strains of salmonella. Pasteurella multocida in most cases were susceptible to all the tested antimicrobials, however 20% of the isolates were resistant to sulfamethoxazole-trimethoprim. Streptococcus suis demonstrated the highest resistance to tetracycline (43%), lincomycin (40%), sulfamethoxazole- -trimethoprim (40%), and erythromycin (30%). Ceftiofur was the most effective against S. suis. Commensal E. coli showed less resistance than enterotoxigenic E. coli, however not less than 25% of isolates were resistant to tetracycline, sulfamethoxazole-trimethoprim and ampicillin. All the tested Enterococcus faecalis were susceptible to vancomycin and ceftiofur and 80% of enterococci were resistant to tetracycline.
8

Tetracycline, erythromycin, and gentamicin resistance of Campylobacter jejuni and Campylobacter coli isolated from poultry in Poland

58%
Wozniak A., Wieliczko A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
The aim of the research was the determination of resistance of 387 Campylobacter sp. strains isolated between 1994 and 1996 (broilers, laying hens) and 2005-2008 (broilers, laying hens, turkeys) to tetracycline, erythromycin, and gentamicin. The resistance was determined with the reference to minimum inhibitory concentration on the agar plates (MIC). Limit concentration value for tetracycline and gentamicin was measured at >8 µg/mL, while for erythromycin it was obtained at >16 µg/mL. Among the Campylobacter sp. isolates obtained in 2005-2008, tetracycline resistant strains were found, while the isolates obtained between 1994 and 1996 were tetracycline susceptible. An increase in the percentage of erythromycin intermediate susceptible strains from 2005- 2008 was reported in comparison to the isolates obtained in 1994-1996. Gentamicin susceptibility was found in 99.5% of all the Campylobacter sp. strains isolated between 1994 and 2008. These findings reveal a broad extent of antimicrobial resistance in Campylobacter isolates from poultry in Poland and underline the need for prudent use of antibiotics in poultry production to minimise the spread of antibiotic resistant Campylobacter strains.
9

Chloramphenicol acetyltransferase activity in E. coli and A. tumefaciens strains harboring plasmids with the enzyme coding sequences

58%
Bartkowiak S., Grygorowicz W., Augustyniak H.
Biological Bulletin of Poznań
|
1994
|
tom 31
10
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Strategies for Helicobacter pylori eradication in 1995: A review of international and Belgian experience

58%
Deltenre M., Jonas C., Otero J., Cozzoli A., Denis P., Burette A., De Koster E.
Journal of Physiology and Pharmacology
|
1996
|
tom 47
|
nr 1
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