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A newly recorded Spiroplasma infection in Stilpnotia salicis L. (Lepidoptera, Lymantriidae)

100%

Lipa J. J., Ziemnicka J., Gazecka B., Korytek L.

Prace Naukowe Instytutu Ochrony Roślin

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1988

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tom 30

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nr 1-2

2

Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test

100%

Kaminski S., Czarnik U.

Journal of Applied Genetics

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1997

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tom 38

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nr 1

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

3

Bacteriophage therapy of bacterial infections: an update of our institute's experience

88%

Weber-Dabrowska B., Mulczyk M., Gorski A.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

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nr 6 Spec.Iss.

547-551

4

MicroRNAs as new immunity regulators in viral and bacterial infections

88%

Szumna M., Hukowska-Szematowicz B.

Acta Biologica

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2020

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tom 27

5

Effect of bathing in a 0.1% aqueous solution of ethacridine lactate on selected physiological parameters of Cornu aspersum Müller edible snails

75%

Zietek J., Dziegiel B., Wojcik A., Wilczynska A., Adaszek L., Winiarczyk S.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

6

Live vaccines in broilers and the human health

75%

Kapitancik B., Jurcina A.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

7

Helicobacter pylori inhibits expression of heat shock protein 70 (HSP70) in human epithelial cell line. Importance of Cag A protein

75%

Targosz A., Pierzchalski P., Krawiec A., Szczyrk U., Brzozowski T., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

Heat shock proteins (HSP) are crucial for the maintenance of cell integrity under normal cell growth and at pathophysiological conditions such as colonization of gastric mucosa by Helicobacter pylori (Hp). The effect of Hp on mRNA expression for HSP70 in the gastric epithelial cells in vitro has been little studied and remains inconclusive. In this study we attempted to determine the alterations in gene expression for HSP70 induced by two live strains of Hp in the epithelial MKN7 cells. The following Hp strains were employed; 1) Hp strain expressing cagA and vacA, and 2) cagA and vacA negative Hp strain without or with addition of exogenous recombinant protein CagA. MKN7 cells were incubated in a standard medium RPMI 1640 supplemented with 10% fetal bovine serum at 37°C with 5% CO2 and humidified atmosphere under basal condition or in a presence of Hp (1x109 CFU per dish) without or with the recombinant CagA (10 µg/ml of RPMI 1640 medium). After 3h, 24h and 48 h of incubation with Hp and in some experiments with the prolonged incubation time up to 72 h, the cells were harvested, the total cellular RNA was isolated and the expression of mRNA for HSP70 was determined by RT-PCR. The incubation of the MKN cells with CagA protein alone failed to affect significantly the expression of HSP70. In contrast, the strain Hp (cagA+, vacA+) inhibited in time-dependent manner the expression of mRNA for HSP70. When the MKN7 cells were coincubated with Hp (cagA+, vacA+) and exogenous CagA, the significant inhibition of the signal intensity for HSP70 mRNA was observed at 3 h and 24 h of incubation and these effects were followed by complete disappearance of the signal for HSP70 mRNA at 48 h. The incubation of MKN7 with Hp (cagA-, vacA-) also significantly attenuated the expression of HSP70 mRNA with the most pronounced inhibitory effect observed at 72 h of incubation with this Hp strain. Addition of the recombinant CagA to Hp (cagA-, vacA-) completely suppressed the expression of HSP70 at 48 h and 72 h after the end of incubation periods. We conclude that 1) both, Hp (cagA+, vacA+) and Hp (cagA-, vacA-) inhibit expression of HSP70 in MKN7 human gastric epithelial cells independently of the presence or absence of cagA gene, and that 2) recombinant CagA protein may exert biological activity in vitro via acceleration of inhibitory effect of Hp negative for Cag A and VacA on HSP70 expression in epithelial cells infected with this bacteria.

8

Interference by leptin with Helicobacter pylori lipopolysaccharide-induced cytosolic phospholipase A2 activation in gastric mucosal cells

75%

Slomiany B. L., Slomiany A.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 1

Activation of cytosolic phospholipase A2 (cPLA2) by bacterial LPS for the rapid release of arachidonic acid from membrane phospholipids is considered a key step in the generation of platelet-activating factor (PAF), recognized as the most proximal mediator of inflammatory events triggered by bacterial infection. In this study, we report on the role of leptin in modulation of the detrimental consequences of H. pylori LPS-induced cPLA2 activation that result in the disturbances in gastric mucin synthesis. Employing gastric mucosal cells labeled with [3H] arachidonic acid, we show that H. pylori LPS-induced cPLA2 activation, associated with up-regulation in apoptosis and PAF generation, and the impairment in gastric mucin synthesis, was subject to a dose-dependent suppression by leptin, as well as the inhibition by MAFP, a specific inhibitor of cPLA2. A potentiation in the countering capacity of leptin on the LPS-induced up-regulation in apoptosis, arachidonic acid release and PAF generation was attained in the presence of ERK inhibitor, PD98059, while PI3K inhibitor, wortmannin had no effect. On the other hand, the prevention by leptin of the LPS detrimental effect on mucin synthesis was subject to suppression by wortmannin, an inhibitor of PI3K as well as the inhibitor of ERK, PD98059. Moreover, potentiation in the effect of leptin on the LPS-induced decrease in mucin synthesis was attained with cPLA2 inhibitor, MAFP as well as PAF receptor antagonist, BN52020. The results of our findings point to H. pylori LPS-induced ERK-dependent cPLA2 activation as a critical factor influencing the level of PAF generation, and hence the extent of pathological consequences of H. pylori infection on the synthesis of gastric mucin. Furthermore, we show that leptin counters the pathological consequences of H. pylori-induced cPLA2 activation on gastric mucin synthesis through the involvement in signaling events controlled by MAPK/ERK and PI3K pathways.

9

Phage therapy: a reappraisal of bacteriophages as antibiotics

75%

Inal J. M.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 4

10

Immunotherapy in Gram-negative bacterial infections

75%

Lugowski C.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

Endotoxins are responsible for initiation of septic shock which increases the number of fatalities in Gram-negative bacteremia among hospital patients. The morality from septic shock is still high despite recent developments in antibiotic therapy because antibiotics are unable to decrease the level of free lipopolysaccharide in the blood stream. Another approach to the treatment and prevention of septicaemia involves stimulation of an immune response against LPS. It was found that immunization with the core structures of endotoxin conjugated with proteins protected animals against infections and endotoxic shock. Anticonjugate sera are of great interest because they are directed against conserved parts of LPS and therefore could have cross-reactive and cross-protective properties with respect to many Gram-negative rods.

11

Phage therapy: past history and future prospects

75%

Carlton R. M.

Archivum Immunologiae et Therapiae Experimentalis

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1999

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tom 47

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nr 5

12

The effect of nitrofurazone and furazolidone on induction of cytochrome P-450 dependent monooxygenase system

75%

Czeczot H., Kobierska-Szeliga M., Bilbin M.

Acta Biochimica Polonica

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1992

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tom 39

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nr 1

13

Differences in the expression of human papillomavirus type 16 (HPV-16) E6 oncogene mRNA in SiHa cell line inoculated with CMV, HSV or ureaplasmas

63%

Szostek S., Zawilinska B., Biernat-Sudolska M., Kopec J., Kleszcz E., Koprynia M., Rojek-Zakrzewska D., Kosz-Vnenchak M.

Folia Biologica

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2014

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tom 62

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nr 1

14

Increased circulating endocan in patients with cirrhosis: relation to bacterial infection and severity of disease

63%

Zuwala-Jagiello J., Simon K., Kukla M., Murawska-Cialowicz E., Gorka-Dynysiewicz J., Grzybek E., Pazgan-Simon M.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 2

15

Siderophore production by Gram-negative rods isolated from human polymicrobial infections

63%

Mokracka J., Cichoszewska E., Kazanowski A.

Biological Letters

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2011

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tom 48

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nr 2

16

Caspases as the key effectors of inflammatory responses against bacterial infection

63%

Uchiyama R., Tsutsui H.

Archivum Immunologiae et Therapiae Experimentalis

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2015

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tom 63

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nr 1

17

The first case of a Staphylococcus pseudintermedius infection after joint prosthesis implantation in a dog

63%

Miedzobrodzki J., Kasprowicz A., Bialecka A., Jaworska O., Polakowska K., Wladyka B., Dubin A.

Polish Journal of Microbiology

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2010

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tom 59

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nr 2

133-135

We have reported a bacterial infection in a dog with progressive dysplasia of the hips. Orthopedic surgery was performed. Seven weeks prior to the surgery, the patient was bitten by another dog. The postimplantation wound exuded for four days after the surgery. Microbiological analysis performed by standard identification techniques showed the presence of Staphylococcus intermedins, but an additional molecular analysis indicated S. pseudintermedius. This was followed by an evaluation of antibiotic susceptibility of the strain which showed cefoxitin, ciprofloxacin, clindamycin, trimethoprim/sulfamethoxazole, doksycycline, erythromycin, and gentamicin resistance. Minimal inhibitory concentration (MIC) values for selected antibiotics were reported. Resistance for cefoxitin indicates that methicillin resistant S. pseudintermedius (MRSP) strains were present in individual macroorganisms, but they can expand and persist the colonization of other hosts.

18

Expression of angiogenesis-stimulating factors (VEGF, CD31, CD105) and angiogenetic index in gingivae of patients with chronic periodontitis

63%

Kasprzak A., Surdacka A., Tomczak M., Przybyszewska W., Seraszek-Jaros A., Malkowska-Lanzafame A., Siodla E., Kaczmarek E.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 4

19

Staphylococcal cassette chromosome mec (SCCmec) classification and typing methods: an overview

63%

Turlej A., Hryniewicz W., Empel J.

Polish Journal of Microbiology

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2011

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tom 60

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nr 2

Meticillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but since late 1990s also the community-acquired. For better understanding of the S. aureus epidemiology there is an urgent need for creation of new typing method for SCCmec element. The molecular typing of MRSA for epidemiological purposes is investigated by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), spa typing and the SCCmec type assignment. In last few years not only new type of SCCmec (VI to XI) have been identified, but also additional subtypes (i.e. IVg-j) and different variants of already existed one (i.e. 5C2&5 and 2B2&5) were discovered. The aim of this review is to briefly summarize current knowledge about SCCmec classification and to discuss advantages and disadvantages of selected SCCmec typing methods.

20

Etiopathogenesis of recurrent aphthous stomatitis and the role of immunologic aspects: literature review

63%

Slebioda Z., Szponar E., Kowalska A.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 3

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