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Endotoxin, a characteristic external fraction of the outer membrane from Gram-negative bacteria, continuously shed into the environment, is considered as an important risk factor for human health. Our purpose was to study the bacterial species contaminating healthy working environments. Airborne, working surfaces and carpet dust samples were collected from 25 offices. Bacterial species were identified with biochemical ApiSystem® strips. Endotoxin concentrations in settled dust were measured with the kinetic chromogenic Limulus assay. The airborne bacterial level varied from 44-2,511, with a median of 277 cfu/m3. Bacterial contamination on surfaces ranged from 1-1000, with 33 cfu/25 cm2 as median value. On carpets, bacterial concentration ranged from 0.73-185 x 105 cfu/g, with 7.28 x 105 cfu/g as median value. Endotoxin concentration varied from 4.6-116.2 EU/mg, with a median of 20.3 EU/mg. Altogether, 501 bacterial strains were isolated. The species variability was greater in Gram-negative bacteria than in Gram-positive cocci with 41 versus 34 various species. In conclusion, people working in healthy offices can be exposed to large concentrations of airborne and dust bacteria and related endotoxin concentrations, giving a risk of work-related diseases.
Background. Bacterial contamination is an ongoing problem for commercial bioethanol plants. It concerns factories using grain and also other raw materials for ethanol fermentation. Bacteria compete with precious yeasts for sugar substrates and micronutrients, secrete lactic and acetic acids, which are toxic for yeast and this competition leads to significant decrease of bioethanol productivity. For this study, bacterial contamination of corn grain was examined. Then the grain was treated by ammonia solution to reduce microbial pollution and after that the microbiological purity of grain was tested one more time. Disinfected and non-disinfected corn grains were ground and fermentation process was performed. Microbiological purity of this process and ethanol yield was checked out. Material and methods. The grain was disinfected by ammonia solution for two weeks. Then the grain was milled and used as a raw material for the ethanol fermentation. The fermentation process was carried out in 500-ml Erlenmeyer flasks. Samples were withdrawn for analysis at 0, 24, 48, 72 hrs. The number of total viable bacteria, lactic acid bacteria, acetic acid bacteria, anaerobic bacteria and the quantity of yeasts and moulds were signified by plate method. Results. Ammonia solution effectively reduces bacterial contamination of corn grain. Mash from grain disinfected by ammonia contains less undesirable microorganisms than mash from crude grain. Moreover, ethanol yield from disinfected grain is at the highest level. Conclusions. The ammonia solution proved to be a good disinfection agent for grain used as a raw material for bioethanol fermentation process
Bacteriophages are an attractive tool for application in the therapy of bacterial infections, for biological control of bacterial contamination of foodstuffs in the alimentary industry, in plant protection, for control of water-borne pathogens, and control of environmental microflora. This review is mainly focused on structures governing phage recognition of host cell and mechanisms of phage adsorption and penetration into microbial cell.
The studies aimed at determining the influence of lactic acid on Salmonella spp. during storage of turkey carcasses’ samples for 2, 4 and 6 days. The initial average contamination of turkey carcasses’ elements with Salmonella spp. was 2.4 · 10³ bacteria. Following the immersion in water in average 4.3 · 10² Salmonella spp. cfu was recovered and that number was assumed as the inoculum. The number of Salmonella spp. decreases during storage of turkey carcasses’ samples in the refrigerator at 4℃. Compared to elements of carcasses immersed in sterile water the largest reduction, by two logarithmic cycles was recorded after 2 days of storage of samples treated with 1% lactic acid. In case of the other variants of the experiment when 1% solution of lactic acid was applied S. Enteritidis grew in numbers within the same logarithmic range. Compared to the samples immersed in sterile water, 2% lactic acid caused reduction in the number of Salmonella spp. on elements of poultry carcasses by one logarithmic cycle both immediately after contamination and after 2 and 6 days of storage; unfortunately after 4 days of storage S. Enteritidis grew in numbers that were within the same logarithmic range. During storage of the turkey samples tested at 4℃ for 2, 4 and 6 days, the numbers of Salmonella spp. decreased. That decrease compared to samples immersed in sterile water was the largest after 2 days of storage after application of 1% lactic acid.
Background. Bacterial contamination is a major problem for commercial fuel ethanol production in distilleries all over the world. The contaminating microorganisms produce acetic and lactic acid that has a detrimental effect on fermentation efficiency. The aim of this work was to calculate the number of bacterial contaminants in some distilleries. Moreover, in this study it was signified what kind of bacteria contaminate ethanol production process. Material and methods. Grains were obtained from five distilleries from some regions in Poland, in this work hereafter referred to as α, β, γ, δ, and ε distilleries. Corn was the raw material in the α, β, and γ distilleries, triticale in δ distillery, and rye in the ε one. From these five distilleries, sweet mashes during fermentation and after it, were also analysed. The total number of microorganisms, the number of lactic acid bacteria, the number of anaerobic bacteria and the quantity of yeasts and moulds in raw materials were calculated. Results. The number of total viable bacteria (CFU/g), lactic acid bacteria (CFU/g), anaerobic bacteria (CFU/g), moulds, and yeasts (CFU/g) occur in the samples were determined. In all distilleries tested, all groups of microorganism were present. Conclusions. The results of our study show that all tested distilleries have a lot of difficulties with microbiology pollution which leads to a decrease of ethanol production and economical problems. From the economical point of view, reduction of microbial contamination makes it possible to increase the production volume
This stiřdy was aimed at determining the influence of three different freezing methods on the horsemeat microfloraduring a 3-month-cold storage. The three methods used included freezing in a container ventilation chamber, freezing in liquid carbon dioxide and two-stage freezing method (combined method based on using liquid carbon dioxide and freezing in a container ventilation chamber). The study revealed that the two-stage freezing method or freezing with liquid carbon dioxide were significantly more effective in the reduction of bacteria within the meat than freezing in a container ventilation chamber. During a 3-month-cold storage of horsemeat, a gradual reduction in bacterial populations was observed. The reduction was significantly greater in the "enriched" meat frozen with the two-stage method or in liquid carbon dioxide as compared to that found in the "normal" meat.
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The aim of this study was bacteriological assessment of water in dental unit reservoirs – concentration and composition of the aerobe and facultative anaerobe bacterial microfl ora. Reservoir water samples were taken from 25 units. Bacterial fl ora were determined with the plate culture method. Bacteria were identifi ed with biochemical microtests: API 20E, API 20NE (bioMérieux, France) and GP2 MicroPlateTM (BIOLOG, USA). The concentration of total bacteria isolated from one site was 201,039 cfu/ml, on average; the minimum was 22,300 cfu/ml, and the maximum – 583,000 cfu/ml. The following bacteria were identifi ed: Gram-negative bacteria – Brevundimonas vesicularis, Moraxella lacunata, Moraxella spp., Ralstonia pickettii, Sphingomonas paucimobilis, Stenotrophomonas maltophilia; Gram-positive cocci – Micrococcus luteus, Micrococcus lylae, Staphylococcus cohnii, Staphylococcus hominis ss novobiosepticus, Staphylococcus spp., Streptococcus spp.; actinomycetes – Streptomyces albus. The prevailing bacteria were: Ralstonia pickettii (96.46%), found in all the units. Sphingomonas paucimobilis (1.32%) and Brevundimonas vesicularis (1.07%) were the next most frequently occurring bacteria. Bacteria concentration in dental unit reservoirs reached excessive values, and the bacterial fl ora were composed of the bacteria characteristic for water supply systems, opportunistic pathogens, and bacteria of the oral cavity fl ora. Continuous microbiological monitoring of the DUWL water, including application of a disinfecting procedure, is necessary.
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