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The paper contains an assessment of the effectiveness of polyaluminium chloride (PAX-18) application in improving sedimentation properties of activated sludge at the wastewater treatment plant in Olsztyn. The causes of bulking and foaming of activated sludge have been identified. The effect of adding doses of polyaluminium chloride on the sludge volume index (SVI) and counts of Microthrix parvicella has been analysed. Application of doses of PAX-18 within the range of 0.63 and 2.13 g Al⁺³ kg⁻¹ sdmd (sludge dry mass daily) to the system, for the duration of 10 to 89 days, proved to be an ineffective way of reducing SVI. However, in most cases, the applied treatments reduced the foaming of activated sludge in multi-functional reactors.
This research was conducted to determine the effects of three grafting methods (chip budding, side and splice grafting) and topical applications on graft scion of IBA (0 and 4000 ppm) and three plant growth promoting rhizobacteria (PGPR) strains (Bacillus subtilus-OSU142, Bacillus megatorium-M3 and Burkholderia gladia-BA7) on graft success and plant growth in black mulberry sapling production. The experiment was carried out in a randomized complete block design with three replications in Bolu, Turkey in 2014 and 2015. The black mulberry (Morus nigra L.) were used for grafting scions. Twoyear old white mulberry (Morus alba L.) seedling were used for rootstocks. The results showed that in general all of the bacterial strains and IBA had significant effects on all parameters tested in graft scion compared with the control. Application of 4000 ppm IBA and Bacillus megatorium-M3 increased the success graft take rate (74.44 and 72.22%, respectively), graft sprouting rate (61.11 and 60.00%, respectively), graft shoot diameter (6.21 and 5.70 mm, respectively) and graft shoot length (35.50 and 35.31 cm, respectively). Grafting methods had significant effect for all parameters and grafting methods increased the graft success. The best graft take rates (75.33 and 70.67%, respectively), graft sprouting rates (62.67 and 57.33% respectively), graft shoot diameter (5.56 and 5.88 mm, respectively) and graft shoot length (34.14 and 37.86 cm, respectively) were obtained from splice grafting and chip budding methods. In conclusion, the present study showed that 4000 ppm IBA and PGPR strains (especially M3 and OSU142) increased the graft success of black mulberry graft. The PGPR application may be of benefit for grafting in mulberry cultivars, particularly for organic farming. Besides, splice grafting and chip budding were found to be successful to black mulberry plant production.
The biotechnical preparations: Biosept Active (based on a grapefruit extract) and BioZell (based on thyme oil) as well as Hypericum perforatum extract, streptomycin solution and fungicide Champion 50WP (active ingredient substance – e.i. 50% copper hydroxide) were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on three media: Nutrient Agar (NA Difco), Pseudomonas Agar F (Merck) – analogue of King B and 523. In the experiments, the agar plate method was applied. There were no statistically significant differences in the effect of streptomycin and Champion 50WP on the growth inhibition of three bacteria strains for medium 523 and Nutrient Agar and of P. syringae pv. syringae and X. arboricola pv. corylina for medium King B. It was determined that the antibacterial activity of Biosept Active and BioZell biopreparations and H. perforatum extract against Agrobacterium tumefaciens (strain C58), Pseudomonas syringae pv. syringae (strain 760) and Xanthomonas arboricola pv. corylina (strain RIPF-x13) were dependent on the strain of pathogen as well as the growth medium used. According to the research results obtained, the Biosept Active preparation and H. perforatum extract demonstrated high bacteriostatic activity against three bacterial strains grown on the Nutrient Agar medium.
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