Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 9

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  apoplast
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Role of the apoplast in Ca2plus metabolism during the pollen-pistil interaction

100%
Lenartowska M., Jaworski K., Lenartowski R., Niedojadlo J., Smolinski D. J., Wrobel B., Bednarska E.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Localization of acid phosphatase activity in the apoplast of root nodules of pea [Pisum sativum]

86%
Sujkowska M., Borucki W., Golinowski W.
Acta Societatis Botanicorum Poloniae
|
2006
|
tom 75
|
nr 1
33-38
Changes in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase activity appears in the outer part of cells of symbiotic bacteria; 2) bacteria show increased AcPase activity, and the enzyme activity appears in the thread walls; 3) activity exhibits also matrix of the infection thread; 4) bacteria just before their release from the infection threads show high AcPase activity; 5) AcPase activity ceases after bacteria transformation into bacteroids. The increase in bacterial AcPase activity may reflect a higher demand for inorganic phosphorus necessary for propagation of the bacteria within the infection threads and/or involved in bacteria release from the infection threads.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Molecular diffusion water exchange in compartments of tissue water of maize

86%
Zaitseva I., Syrovatko V.
International Letters of Natural Sciences
|
2016
|
tom 51
For studying water exchange in compartments of tissue water of maize, kinetic curves of dilution of tritium labeled water (3Н) were used, with the incubation of plant tissues in it. By resolving the summary kinetic curves into components, we determined the constants of membranes’ permeability for exchange diffusion flux in two compartments of tissue water – membranerestricted water and water of higher mobility in free space of cell envelopes, and quantitative content of symplastic and apoplastic water in the plant tissues. Significant differences of rates of water-exchange processes in the symplast and apoplast of maize types with various genotypes were found at the temperatures of 20, 30, 40 and 50°С. In the Pioneer hybrid these figures vary in the intervals of 1,83–3,67 s-1·10-4 and 0,91–1,33 s-1·10-3, whereas in self-pollinated line А-204 the intervals are 1,80–3,51 and 1,12–1,48 accordingly. Peculiar features of water exchange reactions suggest the higher physiological constancy of the Pioneer hybrid under thermal action.
4

Organismal view of a plant and a plant cell

86%
Wojtaszek P.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 2
Cell walls are at the ba sis of a struc tural, four-dimensional frame work of plant form and growth time. Re cent rapid prog ress of cell wall re search has led to the sit u a tion where the old, long-lastingjux ta po si tion: "liv ing" protoplast — "dead" cell wall, had to be dropped. Various at tempts of re-interpretation cast, how ever, some doubts over the very na ture of plant cell and the sta tus of the walls within such a cell. Fol low ing a com par i son of exocellular ma tri ces of plants and an i mals, their po si tion in re la tion to cells and or gan isms is ana lysed. A mul ti tude of per spec tives of the bi o log i cal or gani sa tion of liv ing be ings is pre sented with par tic u lar at ten tion paid to the cel lu lar and organismal the o ries. Ba sic ten ets and re sult ing cor ol lar ies of both the o ries are com­pared, and evolutionary and developmental implications are considered. Based on these data, "The Plant Body" - an organismal con cept of plants and plant cells is de­scribed.
5

Localization of acid phosphatase activity in the apoplast of pea root nodules

72%
Sujkowska M., Borucki W., Golinowski W.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
6

Role of hydrogen peroxide and apoplastic peroxidase in tomato - Botrytis cinerea interaction

72%
Patykowski J.
Acta Physiologiae Plantarum
|
2006
|
tom 28
|
nr 6
The aim of the research was to estimate the sensitivity of tomato tissue and spore from necrotrophic isolate of B. cinerea on H2O2. The influence of exogenic H2O2 and B. cinerea on plant tissue and on the activity of peroxidases (PO), catalase (CAT) and superoxide dismutase (SOD) in apoplastic tomato leaves fraction were investigated. It was proved that 40 mM H2O2 damaged the cells of a host, and inhibited in vitro germination of B. cinerea spores. Complete inhibition of germination was observed after the use 100 mM H2O2. In the presence of spores H2O2 was decomposed to H2O and O2. Trace activity of catalase was observed in a solution of spores used for inoculation. Necrosis which appeared on the leaves after 40 mM H2O2 treatment resembled hypersensitive response. On the leaves pretreated at this concentration the development of infection was observed. The H2O2 concentration harmful for the tissues, stimulated the PO activity measured with NADH - responsible for generation of ˙O₂⁻, as well as with syringaldazine (S) and ferulic acid (FA), substrates characteristic of forms lignifying and strengthening the cell wall. Clear increase in CAT activity, resulting from infection and early pretreatment with H2O2 was obterved in apoplast. No effect on SOD activity was observed. A hypothesis may be put forward, that germinating spores produce enzymes which allow them to decompose H2O2 generated in apoplast, so there is little likelihood that B. cinerea can be directly inhibited by reactive oxygen forms (ROS) during initial stages of infection. Necrotic lesions resembling HR generated by exogenous H2O2 as well as induction of activity of apoplastic plant enzymes, particularly PO connected with strengthening and lignification of cell wall, were not sufficient factors to inhibit fungal expansion.
7

The activity of antioxidative enzymes, contents of H2O2 and of ascorbate in tomato leaves of cultivars more or less sensitive to infection with Botrytis cinerea

58%
Patykowski J., Urbanek H.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 2
The aim of the present studies was to compare H2O2 and ascorbate conients as well as peroxidase (PO) and catalase (CAT) activities in leaves of less susceptible cultivar Perkoz and more susceptible Corindo after B. cinerea infection. Increase in H2O2 contents in both Perkoz and Corindo cytosol was observed, however, it appeared earlier in the less susceptible cultivar. The increase in PO activity in the cytosol fraction was observed 48 hours after infection in both cultivars but it was greater in the less susceptible Perkoz. No significant differences between the tested cultivars were observed in asi corbate peroxidase (APX) activity and in reduced and oxidated ascorbate contents. PO activity was thoroughly analyzed in the apoplast fraction. It was measured with syringaldazine (S), tetramethylbenzidine (TMB) and ferulic acid (FA) - substrates characteristic of isoenzymes involved in lignification and stiffening of a cell wall. Increase in PO activity with these substrates was observed earlier in cultivar Perkoz than in cultivar Corindo. Similarly, increase in PO activity with NADH appeared significantly earlier in cultivar Perkoz. Apoplastic PO was separated with DEAE Sepharose and two fractions binding and non-binding were obtained. Binding PO fraction was significantly more active especially with S, TMB and NADH after B. cinerea infection. The increase in the enzyme activity was mostly observed in cultivar Perkoz. Binding PO was separated by electrophoresis on acrylamide gel and revealed six enzymatic forms from which three were much more active after infection in cultivar Perkoz. The obtained results suggest that cell wall strengthening mediated by apoplast PO is a key factor responsible for different resistance of tomato cultivars Perkoz and Corindo to B. cinerea infection.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Localization of expansin-like protein in apoplast of pea [Pisum sativum L.] root nodules during interaction with Rhizobium leguminosarum bv. viciae 248

58%
Sujkowska M., Borucki W., Golinowski W.
Acta Societatis Botanicorum Poloniae
|
2007
|
tom 76
|
nr 1
During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed.
9

Apoplastic system of the gynoecium and embryo sac in relation to function

58%
Yang H. Y.
Acta Biologica Cracoviensia. Series Botanica
|
2001
|
tom 43
This article addresses the gynoecium and embryo sac from a fresh viewpoint, that is, in terms of the apoplastic system involved in the pistil and its putative functions in sexual processes. This system includes the extracellular matrix of the pollen tube track and particularly the micropyle and synergids, the intercellular space between the cells of the female germ unit, and the apoplast surrounding the embryo sac. Most of the data cited are research results established during the last decade, mainly from ultrastructural and cytochemical observations, which give interesting and important information about the apoplastic system concerned.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.