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Background. The health benefits arising from antioxidant vitamins A, C and E are well recognised and their recommended dietary intake for the general population have been established. However, there is still a need for assessing antioxidant vitamin intake in different population groups. Objective. To assess intake of antioxidant vitamins: A, C, E and β-carotene, and to identify their major sources in the diets of healthy subjects. Material and methods. The study group consisted of 182 adults; both men and women from polish population. Antioxidant vitamin dietary intake was assessed by individual 3-day records. Data were analysed using updated “Polish Food Composition Tables” and ‘Dieta 5’ Software. Results. The average daily intake of antioxidant vitamins was: 1076 mg for vitamin A (including 46% of retinol and 55% of β-carotene), 107 mg for vitamin C and 9 mg for vitamin E. Higher dietary intake of these vitamins was observed in men compared to women. Conclusions. The average intake of antioxidant vitamins was found to be in recommended range, however, significant differences were observed between the lowest and the highest intake. They were related to differences in the consumption of food products recognized as major sources of vitamins A, C, E and β-carotene in study population.
The composition of fatty acids of serum lipids, the levels of serum lipids (total cholesterol, LDL-cholesterol, HDL-cholesterol, VLDL-cholesterol, triglycerides), mineral components (sodium, potassium, calcium, magnesium) and antioxidant vitamins (ascorbic acid, retinol, carotene, tocopherol) were determined in men aged about 25 years. The serum of hypertensive subjects (systolic blood pressure > 140 mmHg or more) contained, in relation to normotensive subjects, significantly less linolic acid, docosahexaenoic acid and oleic acid, but the total level of unsaturated fatty acids was not different between these two groups. No differences were found in the serum levels of the determined mineral components between normotensive and hypertensive men.
Inhalation of high concentration of oxygen produces oxidative stress in men and experimental animals. Our previous experiments showed that the cough reflex is suppressed in guinea pigs after exposure to 100% O2 for 60 hours. The aim of this study was to determine the effects of dietary antioxidant supplementation with vitamins C and E on hyperoxia-induced oxidative stress in airway and lung tissues directed on cough reflex. The experimental group (T-H, n=8) was pretreated with vitamins C (500 mg/kg) and E (300 mg/kg) for 4 weeks and subsequently exposed to 100% O2 for 60 hours. Hyperoxic group (H, n=8) received saline instead of antioxidants and then inhaled 100% O2 for 60 hours. Cough was induced by inhalation of citric acid aerosol in gradually increased concentration (0.05-1.6 M) at the end of antioxidant therapy and then at the end of exposure to 100% O2. Cough was also induced by mechanical stimulation of laryngopharyngeal (LPh) and tracheobronchial (TBr) region in anaesthetized animals just 1 hour after the end of oxygen exposure. Our results showed a tendency to a decrease in citric acid-induced cough in hyperoxic animals and an increase in animals with antioxidant therapy after hyperoxia. Antioxidant therapy significantly unblocked hyperoxia-induced down-regulation of cough (P=0.004). Significant changes also were obtained from mechanically-induced TBr cough [2.5(1-4) vs. 1.0(1-2); P<0.01] between the experimental and hyperoxic (control) animals. In conclusion, our results indicate a protective effect of antioxidant supplementation on oxidant-mediated cough depression.
The presented work defines the influence of technological process on chemical composition of peppers. The technological treatments made in this work were: lyophilization and samples evaporation. Evaporation process was conducted in two conditions of temperature and pressure: I – 35°C and 70 mbar, II – 50°C and 400 mbar. The working material consisted of four pepper cultivars, two sweet: ‘King Artur’ and ‘Red Knight’, and two hot: ‘Capel Hot’ and ‘Robustini’. The changes of vitamins C and E, β-carotene, xanthophylls and phenolic compounds were investigated as processing and cultivar factors. Additionally antioxidant activity of phenolic compounds was evaluated by method with DPPH radical. The extracts prepared in conditions I had a significantly higher concentration of vitamin C, E, β-carotene, total phenol and antioxidant properties than the extracts obtained at the higher temperature and under the higher pressure. It was found that extracts obtained from hot fruits had a higher vitamin E and β-carotene contents than from the sweet ones. However, the extracts from sweet varieties were characterized by higher antioxidant properties and phenolic compounds than the ones obtained from hot peppers.
An tioxidantvita mins, being effective free radical scavengers, can protect cellular DNA from ox i da tive dam age. Therefore, in the pres ent study we re port on the re la- tion ship be tween basal level of 8-oxo-2 -deoxyguanosine in hu man lym pho cyte DNA and the concentration of antioxidant vitamins (A, C and E). The average level of 8-oxo-2 -deoxyguanosine in lymphocytes of the studied group (15 males and 20 fe­males) was 9.57 per 106 dG mol e cules. The en dog e nous level of ascor bic acid (vi ta min C) in the plasma was, on av er age, 56.78 uM, while the mean con cen tra tions of retinol (vi ta min A) and α-tocopherol (vi ta min E) were 1.24 uM and 25.74 uM, re spec tively. No correlations were found between individual 8-oxo-2 -deoxyguanosine levels in lymphocyte DNA and en dog e nous con cen tra tion of the vi ta mins.
Although there are numerous methods for quantitative determination of antioxidative vitamins: C, E, and A, there are no methods favourable for the broadly understood analytic practice – they have various faults and limitations or require expensive apparatus. Therefore, we have elaborated modifications of valuable spectrophotometric methods for determination each of those vitamins, which originally could not be applied for laboratory practice from various regards. They are based on: for vitamin C – colour reaction with periodically prepared phosphotungstate reagent; for vitamin E – colour reaction with batophenanthroline, FeCl3 and H3PO4; for vitamin A – spectrophotometric measurement of extracts of tested samples. Control tests showed complete correctness of analytic parameters obtained with those modifications with preservation of advantages of the original methods. Therefore they can be successfully implemented to the routine clinical analyses’. The elaborated modifications can also be used for determination of the a/m vitamins in foodstuffs, for example: in juices, milk and homogenates or extracts of solid food.
The aim of this study has been to determine the effects of hypertension on plasma lipid profile and the serum level of antioxidant vitamins in tobacco smokers. The study population comprised 62 smokers of whom 35 had hypertension (study group) and 27 were normotensive (control group). The levels of plasma lipids, albumins, uric acid, thiobarbituric acid reacting substances (TBARS) and vitamins A, E, C and ß-carotene were assayed. Cumulated index of antioxidant vitamins (CIAVIT) was also calculated. Total cholesterol (TC) (pO.OOl), LDL cholesterol (pO.OOl), uric acid (p<0.05), TBARS (p<0.05) and triglycerides levels were higher in hypertensive smokers compared to the control group. Hypertensive smokers also had lower HDL cholesterol and vitamin E levels (pO.Ol), as well as the levels of vitamins A, C and ß-carotene and the CIAVIT value. The study group exhibited negative correlation between TBARS and vitamins E levels (r=-0.46) and C (r=-0.33), and between TBARS and CIAVIT values (r=-0.34). The study results indicate that tobacco smoking exacerbates pre-existing lipid profile and antioxidant status abnormalities in hypertensive subjects.
Metodą wywiadu żywieniowego z ostatnich 24-godzin przed badaniem oceniono zawartość witamin antyoksydacyjnych w całodziennych dietach 355 dzieci ze szkół podstawowych Białegostoku i okolic. Wykazano, że tylko zawartość witaminy C w jadłospisach 14-letnich chłopców zamieszkałych w mieście jest w granicach normy zalecanej.
Przeprowadzono ilościową oceną sposobu żywienia i badanie składu ciała u osób z nadwagą i hyperlipidemią w zależności od typu otyłości i typu hiperlipidemii. Stwierdzono spożycie tłuszczu przekraczające 30% energii. Niekorzystnym wynikiem było również niskie spożycie witamin antyoksydacyjnych u niektórych pacjentów. Dotyczyło to głównie kobiet z otyłością gynoidalną. Wyniki pracy wskazują, iż niezbędne jest prowadzenie edukacji żywieniowej u pacjentów ze schorzeniami metabolicznymi.
W patologii zmian miażdżycowych poważną rolą odgrywa niedobór witamin antyoksydacyjnych. Podstawowym źródłem tych witamin powinny być spożywane racje pokarmowe. Celem przeprowadzonych badań było ustalenie na ile całodzienne racje pokarmowe kobiet w wieku podeszłym z chorobą niedokrwienną mięśnia sercowego pokrywają zapotrzebowanie na te witaminy oraz czy wielkość spożycia koreluje z ich surowiczym stężeniem? Stwierdzono niską żywieniową podaż witamin E, C oraz ß-karotenu w racjach pokarmowych chorych, która nie była statystycznie istotnie związana z ich surowiczym stężeniem.
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