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The identification and quantification of phenolic compounds of Stachys lavandulifolia Vahl. var. brachydon Boiss. by LC-MS/MS (Liquid Chromatography- tandem Mass Spectrometry) technique is the main purpose of the current study. The high concentrations of quinic acid (2534± 12 ppb) and chlorogenic acid (1882±92 ppb) were detected by LC-MS/MS.. Another goal of the study is to evaluate the antioxidant activities of both ethanol and aqueous extracts of the plant material. The antioxidant potentials of extracts were determined by using five different in vitro methods including; ABTS (2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH(1,1- diphenyl-2-picryl-hydrazyl), FRAP (Ferric ions Reducing Antioxidant Power), CUPRAC (Cupric ions Reducing Antioxidant Power), and ferric thiocyanate methods. The results revealed that the aqueous and ethanol extracts of S. lavandulifolia leaves have good antioxidant potential with high phenolic content.
The aim of this work was to determine the total phenolic content (TPC) and the antioxidant activity of methanol extracts of Hericium erinaceus, Hericium americanum and Hericium coralloides, including free radical scavenging method (DPPH), ferric reducing antioxidant power (FRAP) and radical cation scavenging method (ABTS). Hericium spp exhibited moderate to high antioxidant activity. The highest TPC (3.27 ±0.01 mg GAE g–1) and antioxidant activity values (17.0 ±0.68 mmol TE g–1 in FRAP; EC 50 = 4.12 ±0.12 mg mL –1 in DPPH •; EC 50= 2.83 ± 0.10 mg mL–1in ABTS•+ ) were found for methanol extracts of H. coralloides. The TPC and antioxidant activity of H. erinaceus isolates varied from strain to strain. H. americanum possessed considerably lower total phenolic content (2.31 ±0.01 mg GAE g–1) and antioxidant activity (10.5 ±0.59 mmol TE g–1 in FRAP; EC 50 = 7.82 ±0.09 mg mL –1 in DPPH•; EC 50 = 6.36 ± 0.12 mg mL–1 in ABTS •+) than H. coralloides and H. erinaceus. A high correlation was determined between TPC and ABTS •+( r2= 0.855), DPPH•(r2= 0.969) and FRAP (r2 = 0.942). According to results obtained in the present study, Hericium spp., especially H. coralloides and some of H. erinaceus isolates, might be promising natural source of antioxidants for food and pharmaceutical industry.
At present, major causes of diseases is oxidative stress affecting both metabolic and physiological functions of the body. That is why there is a great need for investigation of nutritious food supplements for counteracting these oxidative stresses. Therefore, the aim of study was to evaluate the therapeutic potential of Apium leptophyllum Pers. fruits by estimating total phenolic as well as flavonoidal contents and antioxidant values. The collected fruits were extracted separately using different solvents like methanol, ethanol and water. Total phenolic and flavonoid contents were measured from the respective extracts and correlated with their antioxidant values. The antioxidant properties of various fruit extracts (12.5, 25, 50, 100 and 150 μg/ml) were evaluated by DPPH, hydroxyl, nitric oxide and superoxide scavenging assay and compared with ascorbic acid as a standard. All the extracts of A. leptophyllum were found to be dose dependent inhibition against these free radicals. Among all these extracts, the methanolic one was found better in the scavenging activity and followed dose-dependent manner against DPPH, hydroxyl radical, nitric oxide, superoxide anions with minimum IC50 values of 97.9, 89.02, 135.37, 127.73 μg/ml, respectively, and also observed more significant (p<0.01) as compared with standard. Furthermore, total phenolic and flavonoidal contents were found highest in methanolic extract. The results obtained in this study clearly indicate that the methanolic extract of A. leptophyllum may be used as a new potential source of natural nutritional supplement in food or pharmaceutical industries due to rich source of phenolic, flavonoidal contents as well as antioxidant property.
The aim of the study was to evaluate the antioxidative potential of blood during standard physical exercise of horses. The study included 114 clinically healthy horses representing different groups: breeding horses (27), recreation horses (22), and sport horses (65). The group of sport horses consisted of race horses (11), trotters (15), and jumping (25) and driving horses (14). The blood was collected from external jugular vein three times: before exercise, immediately after exercise, and after 30-min rest. The following enzymatic and nonenzymatic antioxidative indices were determined: superoxide dismutase, glutathione peroxidase (GPx), catalase, albumin, total bilirubin, uric acid, and total antioxidant status (TAS). The study demonstrated a temporary post exercise mobilisation of antioxidative mechanisms, especially in cases of intensively trained competitive horses. It was demonstrated that among the antioxidants, the activity of GPx showed the high post-exercise changeability, which suggests a great importance of this enzyme in the protection of the organism from the increased generation of reactive oxygen species. The analysis of results of pre- exercise examination indicated higher rest values of main antioxidative enzymes and TAS in horses trained regularly and intensively than those in animals of a small physical activity. These results prove the positive influence of training on antioxidative potential of blood in horses.
The antioxidant capability and phenolic contents of ethanol extracts (free phenolics) and ethyl acetate extracts (bound phenolics) of three Quercus species were estimated in this work. The antioxidant activities were examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical, reducing power and β-carotene bleaching methods. HPLC was employed to detect major phenolic acids. The leaf extract of Q. salicina contained maximum total phenolics while the highest total flavonoid content was found in the leaf extract of Q. serrata. The antioxidant activities varied among three species. Bark extract of Q. salicina was the most potential and it was closed to levels of the standard antioxidative dibutyl hydroxytoluene (BHT). The bark extract of Q. serrata also showed promising antioxidant activities despite their eminence was negligibly lower than Q. salicina. Stronger antioxidant activities of free phenolics than those of the bound phenolics may be attributed to higher quantities of free phenolics in the barks of Quercus species, however total flavonoids may not contribute a critical role. By HPLC analysis, thirteen phenolic acids were detected in the leaf and bark extracts. Of them, Q. salicina showed maximum in number (ten compounds) and quantities of detected phenolic acids. Ellagic, chlorogenic and benzoic acids were dominant in Quercus species. Findings of this study revealed that leaves and barks of three Quercus species are rich source of antioxidants, and Q. salicina is the most promising and should be elaborated to exploit its pharmaceutical properties.
Three approaches were successfully used to manipulate content of flavonoids in transgenic plants. Overexpressing either the adaptor 14-3-3 protein or genes coding the key enzymes of the flavonoid biosynthesis pathway resulted in a significant increase in the compound content in potato tuber epidermis. The opposite effect was observed in transgenic plants in which these proteins were repressed; this strongly supports the view that the gene construct deiermines transgenic plant feaiures. The most effective construct was, however, the one containing single dihydroflavonol reductase (DFR) gene in sense orientation. In all cases the increase in flavonoid content resulted in the expected enhancement of the antioxidant capacity of tuber extract. At the biochemical level a decrease in the starch content in transgenic plant overexpressing proteins regulating flavonoid biosynthesis was detected. In the case of glucosyl transferase (GT) gene overexpression, the content of phenolic compounds remained at the control level, however, the antioxidant capacity of tuber extracts significantly decreased. The GT plants grew faster glucosylation of flavonoids rather than their quantity which determines transgenic plant features.
Decalepis hamiltonii Wight & Arn., is a plant species that is endemic to southern parts of India. The aim of this study is to explore the influence of habitat heterogeneity on total phenolics, flavonoids, flavor compound 2-hydroxy-4-methoxy benzaldehyde (2H4MB) and antioxidant potential of tubers. The flavor metabolite 2H4MB was quantified by HPLC using isocratic solvent system (methanol : acetonitrile : water : acetic acid 47 : 10 : 42 : 1) that indicates obvious difference in 2H4MB content of tubers with a maximum of 96.4 ±2.6 and 92.6 ±1.2 mg 100 g–1 dry weight basis (DW) in samples from B.R. Hills and Mysore area of Karnataka, followed by samples from Tirumalai Hills and Kurnool from Andhra Pradesh (89.02 ±0.9 mg 100 g–1 DW), Tamil Nadu (81.6 ±2.4 mg 100 g–1 DW) and Kerala (80.18 ±1.1 mg 100 g–1 DW) of tubers. There was variation in total phenolics, total flavonoids and 2H4MB content of root samples collected from different habitats. Also significant variation in free radical scavenging potential of methanol root extracts was noticed, which is directly proportional to the phenolics, and flavonoids content. Overall, there was 10–16% difference in content of 2H4MB in D. hamiltonii tubers that were collected from different natural habitats, and this habitat heterogeneity has to be considered vital, while using such tubers for edible purposes and food formulations.
The purpose of this study was to design the formula and antioxidant potential of gluten-free oat drinks fortifi ed with bioactive plant components. The paper describes the use of three preparations of plant origin: yellow tea leaf extract, mulberry extract and steviol glycosides from stevia leaves. Analyses of the oat drinks basic composition – including proteins, lipids, carbohydrates, dietary fi ber fractions and antioxidant properties with DPPH, ABTS, ORAC and FRAP assays were conducted. Tea extract with steviosides oat drink showed the highest total phenolic concentration and the highest antioxidant activity followed by stevia and mulberry oat drink, whereas stevia oat drink showed the lowest phenolics level and weakest antioxidant activity. Moreover, the applied plant components showed moderate ability to influence the sensory values of the fortifi edoat drinks. The results demonstrate that tea, stevia and mulberry preparations applied in oat drinks enhanced antioxidant potential and might be a natural source of antioxidant components.
Żywność ekologiczna cieszy się coraz większym zainteresowaniem wśród konsumentów, ponieważ jest uważana za bardziej bezpieczną i wartościową pod względem jakościowym. W wielu badaniach dowiedziono, że warzywa i owoce z produkcji ekologicznej wykazują zazwyczaj korzystniejsze parametry jakości zdrowotnej i odżywczej niż płody konwencjonalne, natomiast brakuje badań dotyczących jakości przetworów owocowych i warzywnych, wykonanych z surowców ekologicznych. Dlatego w pracy porównano wybrane cechy wartości odżywczej przetworów z jabłek ekologicznych i konwencjonalnych (soków i kremogenów). Przetwory (sok i kremogen) wyprodukowane z jabłek ekologicznych charakteryzowały się istotnie wyższą zawartością polifenoli oraz wyższą aktywnością przeciwutleniającą niż wykonane z jabłek konwencjonalnych. Świadczy to o potencjalnie korzystnej wartości biologicznej przetworów ekologicznych. Proces pasteryzacji oraz przechowywania przetworów spowodował zmniejszenie zawartości polifenoli ogółem oraz aktywności przeciwutleniającej soku i kremogenu jabłkowego. Największą zawartość polifenoli oraz aktywność przeciwutleniającą stwierdzono w przetworach z jabłek odmiany Jonagold, pośrednią w produktach z jabłek odmiany Cortland, a najmniej tych związków wystąpiło w soku i kremogenie z jabłek odmiany Idared.
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