Wyniki wyszukiwania - AGRO

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Antibiotic resistance of bacteria of the family Enterobacteriaceae isolated from vegetables - short report

100%

Laniewska-Trokenheim L., Sobota M., Warminska-Radyko I.

Polish Journal of Food and Nutrition Sciences

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2006

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tom 15/56

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nr 4

Antibiotic resistance of 114 strains of the Enterobacteriaceae family bacteria isolated from vegetables, originating from retail, was investigated in the study. The highest number of the strains isolated were resistant to ampicilin (81.9%), whereas a lower number of the strains exhibited resistance to the following antibiotics: neomycin (29.3%), streptomycin (28.4%), rifampicin (21.5%), chloramphenicol (19.8%), colistin (12.9%), and nitrofurantoin (11.%). All the isolated strains appeared to be susceptible to vancomycin, kanamycin, doxycyclin, nalidixic acid and gentamycin.

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Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Meropenem resistance in intensive care units in Ukraine

88%

Oliynyk O., Slifirczyk A., Pereviznyk B.

Health Problems of Civilization

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2018

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tom 12

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nr 2

The article reviews the issue of antibiotic resistance of microorganisms to meropenem in intensive care units in Ukraine. An increase in meropenem inefficiency against microorganisms in intensive care units has been observed in the last years. The data analysis suggests a significant predominance of gram-negative flora: A. baumannii, Р. aeruginosa, К. pneumoniae, E. cloacae, E.coli. On average 30% of microorganisms are resistant to 5 and more basic antibiotics including meropenem. 40-80 % of the gram-negative flora in intensive care units in Ukraine are resistant to meropenem. This can be attributed to the free sale of antibiotics without prescription, patients’ self-treatment, inadequate antibiotic therapy, and failure to comply with sanitary norms on the part of intensive care staff. Microbiological diagnostics of infectious pathogens also needs improvement. Unless proper measures are taken within a few years, meropenem as an antibiotic is likely to disappear in Ukraine.

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Threats to aquatic ecosystems caused by antibiotic-resistant isolate of Escherichia coli from sewage

88%

Frak M., Kozerska Z.

Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation

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2016

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tom 48

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nr 4

4

Antibiotic resistance and siderophores production by clinical Escherichia coli strains

88%

Khazaal M. T., El-Hendawy H. H., Mabrouk M. I., Faraag A. H. I., Bakkar M. R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2022

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tom 103

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nr 2

5

Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production

88%

Simonova M., Laukova A.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 4

6

Antimicrobial resistance of Salmonella isolated from animals and feed in Poland

88%

Wasyl D., Hoszowski A.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

7

Antibiotic resistance of lactic acid bacteria from canine faeces

88%

Strompfova V., Laukova A.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

8

Colistin resistance of non-pathogenic strains of Escherichia coli occurring as natural intestinal flora in broiler chickens treated and not treated with colistin sulphate

75%

Majewski M., Lukomska A., Wilczynski J., Wystalska D., Racewicz P., Nowacka-Woszuk J., Pszczola M., Anusz K.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

9

Prokaryotic toxin-antitoxin systems - the role in bacterial physiology and application in molecular biology

75%

Bukowski M., Rojowska A., Wladyka B.

Acta Biochimica Polonica

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2011

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tom 58

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nr 1

Bacteria have developed multiple complex mechanisms ensuring an adequate response to environmental changes. In this context, bacterial cell division and growth are subject to strict control to ensure metabolic balance and cell survival. A plethora of studies cast light on toxin-antitoxin (TA) systems as metabolism regulators acting in response to environmental stress conditions. Many of those studies suggest direct relations between the TA systems and the pathogenic potential or antibiotic resistance of relevant bacteria. Other studies point out that TA systems play a significant role in ensuring stability of mobile genetic material. The evolutionary origin and relations between various TA systems are still a subject of a debate. The impact of toxin-antitoxin systems on bacteria physiology prompted their application in molecular biology as tools allowing cloning of some hard-to-maintain genes, plasmid maintenance and production of recombinant proteins.

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Inhibitory effect of antibiotics on the growth of heterotrophic bacteria inhabiting marine beach

75%

Perlinski P., Mudryk Z.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2009

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tom 13 part II

The objective of the present study was to evaluate heterotrophic bacteria capable of growth in the presence of different antibiotics and their mixture in such dynamic ecosystem as marine beach. Mixture antibiotics had the strongest inhibitory effect on the growth of bacteria inhabiting sand of studied beach. Culturable bacteria were more resistant to ampicillin than to novobiocin and tetracycline. Inhibitory influence antibiotics on growth bacteria inhabiting studied beach were in the following order: mixture antibiotics > novobiocin > tetracycline > ampicillin.

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Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

75%

Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius- specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

12

Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results

75%

Libisch B., Kereszteny T., Kerenyi Z., Kocsis R., Sipos R., Papp P. P., Olasz F.

Journal of Veterinary Research

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2020

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tom 64

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nr 1

13

Carriage of group B streptococci in pregnant women from the region of Krakow and their antibiotic resistance in the year 2008–2012

75%

Brzychczy-Wloch M., Ochonska D., Bulanda M.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

The aim of the study was a retrospective analysis of the frequency of group B streptococci (Streptococcus agalactaie; GBS) carriage in pregnant women from the region of Krakow, together with an analysis of their drug resistance, carried out between 2008-2012. The study included 3363 pregnant women between 35 and 37 weeks of gestation, studied in accordance with the guidelines of the Polish Gynecological Society (2008). A high percentage of pregnant women who are carriers of group B streptococci was demonstrated. Each year covered by the study, it was in the range of 25-30%, with an average value equal to 28%. The results confirm the need for taking swabs from both the vagina and anus, since 15% of GBS-positive patients showed only rectal carriage. High percentage of isolates resistant to erythromycin was detected, which ranged from 22% to 29%, with an average value equal to 25%, as well as a high proportion of isolates resistant to clindamycin being 17-25%, with an average of 20%. The results indicate the need to standardize the methodology of collecting samples for GBS testing and introduce microbiological diagnostic standards in all gynecological and obstetric centers in Poland, in order to carry out a detailed epidemiological analysis in our country.

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Prevalence and characterisation of class 1 and 2 integrons in multi-drug resistant Staphylococcus aureus isolates from pig farms in Chongqing, China

75%

Ye C., Hou F., Xu D., Huang Q., Chen X., Zeng Z., Peng Y., Fang R.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

15

Serotypes and antibiotic resistance of Streptococcus pneumoniae from adenoids in preschool children with recurrent upper respiratory tract infections

75%

Korona-Glowniak I., Niedzielski A., Malm A., Niedzielska G.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

We investigated children aged 2-5, who had gone adenoidectomy for recurrent and/or persistent symptoms of upper respiratory tract infections for prevalence of pneumococci in adenoid tissue. Serotypes and antibiotic resistance patterns of the isolated pneumococci were determined and also risk factors of pneumococcal colonization were defined. S. pneumoniae colonization in adenoids was found in 62 (60.2%) children. Serotypes belonged to 10-valent and 13-valent pneumococcal conjugated vaccines (PCVs) constituted 56.1% and 68.2% of the isolates, respectively. Decreased susceptibility to penicillin was found in 45.5% of isolates; pneumococci were resistant to co-trimoxazole (62.1%), tetracycline (43.9%), erythromycin (54.5%), clindamycin (54.5%) and chloramphenicol (31.8%). Multidrug resistant S. pneumoniae comprised 57.6% of the isolates. Antibiotic resistant pneumococci were mostly distributed among serotypes belonged to 10-valent and 13-valent PCVs. Good vaccine coverage among the isolated pneumococci confirmed that the introduction of PCVs in the national immunization programme may reduce the pool of resistant and multidrug resistant pneumococci in a community.

16

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

75%

Kmet V., Drugdova Z., Kmetova M., Stanko M.

Annals of Agricultural and Environmental Medicine

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2013

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tom 20

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nr 2

With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

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Glucoprotamin antimicrobial activity against selected standard antibiotic-resistant bacteria and reference strains used in the assessment of disinfection efficacy

75%

Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.

Roczniki Państwowego Zakładu Higieny

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2015

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tom 66

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nr 3

Background. The ability of bacteria to develop common mechanisms of resistance to antibiotics and disinfectants raises doubts about the effectiveness of disinfection processes. Glucoprotamin (GP) is an antimicrobial active substance which is widely used to the disinfection in medical area. Objective. The aim of study was to compare GP’s effectiveness with susceptibility of reference strains used for the evaluation of bactericidal efficacy of disinfectants Staphylococcus aureus (S. aureus); Pseudomonas aeruginosa (P. aeruginosa) and standard antibiotic-resistant strains: meticillin-resistant S. aureus (MRSA) and tetracycline-resistant P. aeruginosa (PAO-LAC). Materials and Methods. Minimum inhibitory concentrations (MICs) of GP and minimum bactericidal concentrations (MBCs) against tested strains were evaluated by serial broth dilution technique. GP’s efficiency was examined according to qualitative (phenol coefficient GP-PC) and quantitative (EN 1040: 2006) test methods. Results. Gram-negative strains were more tolerant to GP than Gram-positive strains among tested strains. MRSA and S. aureus exhibited similar susceptibility to GP. PAO-LAC had significantly lower susceptibility to GP than P. aeruginosa (P≤0,05). There were no differences in GP efficiency against these strains based on GP-PC. According to PN-EN 1040: 2006 standard average obligatory reduction ≥ 5 log10, was demonstrated in the active concentration of GP (84 mg/l) at obligatory 5 min contact time for PAO-LAC and P. aeruginosa. The differences in basis bactericidal activity between PAO-LAC and P. aeruginosa were obtained in the active concentration at 10 and 15 min contact time (P≤0,05). Conclusions. Variation in a susceptibility of reference strains and antibiotic-resistant standard strains has no meaning at used clinically GP concentrations, which are higher than concentration causing basis bactericidal activity of GP.

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Staphylococcus spp. from wild mammals in Aragon (Spain): antibiotic resistance status

75%

Garcia L. A., Torres C., Lopez A. R., Rodriguez C. O., Espinosa J. O., Valencia C. S.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

19

Identification of plasmids in Pseudomonas aeruginosa using agarose gel electrophoresis

75%

Wolska K., Bukowski K.

Polish Journal of Veterinary Sciences

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2001

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tom 04

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nr 2

20

Microbiological contamination of air within the City of Torun

75%

Donderski W., Walczak M., Pietrzak M.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 2

Research was conducted for the present paper on microbiological contamination of the air within the City of Toruń. It follows from the studies conducted that microorganisms occurred in greater numbers in the air within the area of the Old Town than in the Rubinkowo residential district. The number of microorganisms in the air was subject to seasonal changes. The highest numbers of heterotrophic mesophylic bacteria (CFU 37°C) were found in the summer season and the lowest in the winter season. The majority of isolated bacteria belonged to Gram positive forms - bacilli or cocci. A significant proportion of the isolated bacteria displayed antibiotic resistance to commonly used antibiotics.

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